Parasites, Hosts and Diseases

"pork"

Original Article

Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets: Won Gi Yoo, Sun-Min Kim, Eun Jeong Won, Ji-Yun Lee, Fuhong Dai, Ho Choon Woo, Ho-Woo Nam, Tae Im Kim, Jeong-Hee Han, Dongmi Kwak, Yun Sang Cho, Seung-Won Kang, Tong-Soo Kim, Xing-Quan Zhu, Chunren Wang, Heejeong Youn, Sung-Jong Hong; Korean J Parasitol 2018;56(5):437-446.
Published online October 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.5.437

Abstract
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To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066-0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.

Citations

Citations to this article as recorded by

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Dongmi Kwak, Min-Goo Seo
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S. Almeria, J.P. Dubey
Research in Veterinary Science.2021; 135: 371. CrossRef
Descriptive Comparison of ELISAs for the Detection of Toxoplasma gondii Antibodies in Animals: A Systematic Review
K. L. D. Tharaka D. Liyanage, Anke Wiethoelter, Jasmin Hufschmid, Abdul Jabbar
Pathogens.2021; 10(5): 605. CrossRef
All about Toxoplasma gondii infections in pigs: 2009–2020
Jitender P. Dubey, Camila K. Cerqueira-Cézar, Fernando H.A. Murata, Oliver C.H. Kwok, Dolores Hill, Yurong Yang, Chunlei Su
Veterinary Parasitology.2020; 288: 109185. CrossRef
WITHDRAWN: All about Toxoplasma gondii infections in pigs: the past decade
Jitender P. Dubey, Camila K. Cerqueira-Cézar, Fernando H.A. Murata, Oliver C.H. Kwok, Dolores Hill, Yurong R. Yang, Chunlei Su
Veterinary Parasitology.2020; : 109182. CrossRef

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4 Web of Science
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Letter to the Editor

Historical Details about the Meat Consumption and Taeniases in Joseon Period of Korea: Dong Hoon Shin, Jong-Yil Chai, Jong Ha Hong, Min Seo; Korean J Parasitol 2017;55(4):457-460.
Published online August 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.4.457

Previous paleoparasitological studies of Joseon specimens established that the prevalence of Taenia infection was not much different from that of the early 20th century Korean population. As many of taeniases originally diagnosed as Taenia saginata in South Korea were revealed to be actually Taenia asiatica, which share a common intermediate host with T. solium (the pig), Joseon people must have ingested raw pork frequently. However, the current examination of extant Joseon documents revealed that the population ate significant amounts of beef even if the beef ban was enforced; and pork was not consumed as much as we thought. Considering the meat consumption pattern at that time, Joseon people should have been infected by T. saginata more frequently than T. asiatica. This may suggest a low prevalence of T. saginata metacestodes in cattle compared to that of T. asiatica metacestodes in pigs, possibly due to the traditional way of rearing pigs (using human feces). This letter gives us a chance to reconsider the existing preconception about parasitic infections in Korean history though we are still hard to accurately estimate the historical patterns of taeniases at this stage.

Citations

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Piers D. Mitchell
Journal of Archaeological Science.2024; 170: 106067. CrossRef
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Jieun Kim, Jong Ha Hong, Sun Kim, Dong Hoon Shin
Ancient Asia.2022;[Epub] CrossRef
Animal Bones Found at Gongpyeong-dong Archaeological Site, the Capital Area of Joseon Dynasty Period
Sun Kim, Jong Ha Hong, Dong Hoon Shin
Anatomy & Biological Anthropology.2020; 33(2): 99. CrossRef
A comparison of ancient parasites as seen from archeological contexts and early medical texts in China
Hui-Yuan Yeh, Xiaoya Zhan, Wuyun Qi
International Journal of Paleopathology.2019; 25: 30. CrossRef
Prevention and Control Strategies for Parasitic Infections in the Korea Centers for Disease Control and Prevention
Young Yil Bahk, Eun-Hee Shin, Shin-Hyeong Cho, Jung-Won Ju, Jong-Yil Chai, Tong-Soo Kim
The Korean Journal of Parasitology.2018; 56(5): 401. CrossRef

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135 Download
5 Web of Science
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Case Report

An Outbreak of Trichinosis with Molecular Identification of Trichinella sp. in Vietnam: Nguyen Van De, Nguyen Vu Trung, Nguyen Hong Ha, Vu Thi Nga, Nguyen Minh Ha, Pham Thanh Thuy, Le Van Duyet, Jong-Yil Chai; Korean J Parasitol 2012;50(4):339-343.
Published online November 26, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.4.339

Abstract
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The 5th outbreak of trichinosis occurred in a mountainous area of North Vietnam in 2012, involving 24 patients among 27 people who consumed raw pork together. Six of these patients visited several hospitals in Hanoi for treatment. Similar clinical symptoms appeared in these patients within 5-8 days after eating infected raw pork, which consisted of fever, muscle pain, difficult moving, edema, difficult swallowing, and difficult breathing. ELISA revealed all (6/6) positive reactions against Trichinella spiralis antigen and all cases showed positive biopsy results for Trichinella sp. larvae in the muscle. The larvae detected in the patients were identified as T. spiralis (Vietnamese strain) by the molecular analysis of the mitochondrial cytochrome c oxidase subunit III (cox3) gene.

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