Parasites, Hosts and Diseases

"promastigote"

Original Articles

Tamoxifen Induces Apoptosis of Leishmania major Promastigotes in Vitro: Masoud Doroodgar, Mahdi Delavari, Moein Doroodgar, Ali Abbasi, Ali Akbar Taherian, Abbas Doroodgar; Korean J Parasitol 2016;54(1):9-14.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.9

Abstract
PDF

Tamoxifen is an antagonist of the estrogen receptor and currently used for the treatment of breast cancer. The current treatment of cutaneous leishmaniasis with pentavalent antimony compounds is not satisfactory. Therefore, in this study, due to its antileishmanial activity, effects of tamoxifen on the growth of promastigotes and amastigotes of Leishmania major Iranian strain were evaluated in vitro. Promastigotes and amastigotes were treated with different concentrations (1, 5, 10, 20, and 50 μg/ml) and time periods (24, 48, and 72 hr) of tamoxifen. After tamoxifen treatment, MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5 biphenyl tetrazolium bromide assay) was used to determine the percentage of live parasites and Graph Pad Prism software to calculate IC₅₀. Flow cytometry was applied to investigate the induction of tamoxifen-induced apoptosis in promastigotes. The half maximal inhibitory concentration (IC₅₀) of tamoxifen on promastigotes was 2.6 μg/ml after 24 hr treatment. Flow cytometry analysis showed that tamoxifen induced early and late apoptosis in Leishmania promastigotes. While after 48 hr in control group the apoptosis was 2.0%, the 50 ?g/L concentration of tamoxifen increased it to 59.7%. Based on the in vitro antileishmanial effect, tamoxifen might be used for leishmaniasis treatment; however, further researches on in vivo effects of tamoxifen in animal models are needed.

Citations

Citations to this article as recorded by

Therapeutic effect of topical tamoxifen in murine cutaneous leishmaniasis caused by Leishmania major
Parastoo Hassani-Abharian, Seyedamirmehdi Hejazi Dehaghani, Zabihollah Shahmoradi, Seyed Hossein Hejazi, Audrey Odom John
Antimicrobial Agents and Chemotherapy.2024;[Epub] CrossRef
Compared Antileishmanial Activity of Clomiphene and Tamoxifen
Sergio Sifontes-Rodríguez, Alma Reyna Escalona-Montaño, Ricardo Mondragón Flores, Niurka Mollineda-Diogo, Lianet Monzote Fidalgo, Mónica Edith Mondragón-Castelán, Fedra Alardin-Gutiérrez, Lourdes Araceli López-Enzana, Daniel Andrés Sánchez-Almaraz, Ofelia
Biomedicines.2024; 12(10): 2290. CrossRef
Caryocar coriaceumWittm. fruit extracts asLeishmaniainhibitors:in-vitroandin-silicoapproaches
Fernanda Tomiotto-Pellissier, Daniela Ribeiro Alves, Selene Maia de Morais, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Taylon Felipe Silva, Eliandro Reis Tavares, Lucy Megumi Yamauchi, Idessania Nazareth Costa, Emmanuel Silva Marinho, Mar
Journal of Biomolecular Structure and Dynamics.2022; 40(17): 8040. CrossRef
Antileishmanial Activity of Tamoxifen by Targeting Sphingolipid Metabolism: A Review
Kaleab Alemayehu Zewdie, Haftom Gebregergs Hailu, Muluken Altaye Ayza, Bekalu Amare Tesfaye
Clinical Pharmacology: Advances and Applications.2022; Volume 14: 11. CrossRef
The potential role and apoptotic profile of three medicinal plant extracts on Leishmania tropica by MTT assay, macrophage model and flow cytometry analysis
Mozhde Ilaghi, Iraj Sharifi, Fariba Sharififar, Fatemeh Sharifi, Razieh Tavakoli Oliaee, Zahra Babaei, Manzume Shamsi Meimamandi, Alireza Keyhani, Mehdi Bamorovat
Parasite Epidemiology and Control.2021; 12: e00201. CrossRef
Apoptosis-Like Cell Death in Leishmania major Treated with HESA-A: An Herbal Marine Compound
Jasem Saki, Khalil Saki, Reza Arjmand
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Aakriti Garg, Arti Singh, Anoop Kumar
Future Microbiology.2021; 16(13): 987. CrossRef
Network-Based Approaches Reveal Potential Therapeutic Targets for Host-Directed Antileishmanial Therapy Driving Drug Repurposing
J. Eduardo Martinez-Hernandez, Zaynab Hammoud, Alessandra Mara de Sousa, Frank Kramer, Rubens L. do Monte-Neto, Vinicius Maracaja-Coutinho, Alberto J. M. Martin, Tim Downing
Microbiology Spectrum.2021;[Epub] CrossRef
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PLOS Neglected Tropical Diseases.2020; 14(1): e0007995. CrossRef
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Aakriti Garg, Balraj Singh, Ruchika Sharma, Arti Singh, Anoop Kumar
Current Molecular Medicine.2020; 20(2): 102. CrossRef
Epidemiology, Associated Factors and Treatment Methods of Cutaneous Leishmaniasis Based on Previous Data from 2013 to 2018 in Ilam, Western Iran
Nasrin Rezaee, Vahid Raissi, Ahmad Rajaeipour, Mehdi Nazari, Muhammad Getso, Ali Taghipour, Omid Raiesi, Asmaa Ibrahim
Acta Parasitologica.2020; 65(3): 760. CrossRef
Diethyldithiocarbamate encapsulation reduces toxicity and promotes leishmanicidal effect through apoptosis-like mechanism in promastigote and ROS production by macrophage
João Paulo Assolini, Fernanda Tomiotto-Pellissier, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Claudia Stoeglehner Sahd, Amanda Cristina Machado Carloto, Paulo Emilio Feuser, Arthur Poester Cordeiro, Sergio Marques Borghi, Waldiceu Apareci
Journal of Drug Targeting.2020; 28(10): 1110. CrossRef
4-nitrochalcone exerts leishmanicidal effect on L. amazonensis promastigotes and intracellular amastigotes, and the 4-nitrochalcone encapsulation in beeswax copaiba oil nanoparticles reduces macrophages cytotoxicity
João Paulo Assolini, Thais Peron da Silva, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Fernanda Tomiotto-Pellissier, Claudia Stoeglehner Sahd, Amanda Cristina Machado Carloto, Paulo Emilio Feuser, Arthur Poester Cordeiro, Claudia Sayer, Pe
European Journal of Pharmacology.2020; 884: 173392. CrossRef
Repurposing azole antifungals into antileishmanials: Novel 3-triazolylflavanones with promising in vitro antileishmanial activity against Leishmania major
Masoud Keighobadi, Saeed Emami, Mahdi Fakhar, Azar Shokri, Hassan Mirzaei, Saeed Hosseini Teshnizi
Parasitology International.2019; 69: 103. CrossRef
Study of Ethinyl Estradiol Activity Against Promastigotes, Axenic and Macrophage-Dwelling Amastigotes of Leishmania infantum by Using Atomic Force Microscopy and Methyl Thiazolyl Tetrazolium Methods
Farnoush Shadnoush, Reza Arjmand, Fakher Rahim, Jasem Saki
Jundishapur Journal of Microbiology.2019;[Epub] CrossRef
Repurposing as a strategy for the discovery of new anti-leishmanials: the-state-of-the-art
REBECCA L. CHARLTON, BARTIRA ROSSI-BERGMANN, PAUL W. DENNY, PATRICK G. STEEL
Parasitology.2018; 145(2): 219. CrossRef
Caryocar coriaceum extracts exert leishmanicidal effect acting in promastigote forms by apoptosis-like mechanism and intracellular amastigotes by Nrf2/HO-1/ferritin dependent response and iron depletion
Fernanda Tomiotto-Pellissier, Daniela Ribeiro Alves, Milena Menegazzo Miranda-Sapla, Selene Maia de Morais, João Paulo Assolini, Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Allan Henrique Depieri Cataneo, Danielle Kian, Tiago Bervelieri Ma
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Epidemiological Aspects of Cutaneous Leishmaniasis during 2009-2016 in Kashan City, Central Iran
Doroodgar Moein, Doroodgar Masoud, Mahboobi Saeed, Doroodgar Abbas
The Korean Journal of Parasitology.2018; 56(1): 21. CrossRef
Tamoxifen Never Ceases to Amaze: New Findings on Non-Estrogen Receptor Molecular Targets and Mediated Effects
Tatiana Anatolievna Bogush, Boris Borisovich Polezhaev, Ivan Andreevich Mamichev, Elena Alexandrovna Bogush, Boris Evseevich Polotsky, Sergei Alexeevich Tjulandin, Andrey Borisovich Ryabov
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Cell death mechanisms in Leishmania amazonensis triggered by methylene blue-mediated antiparasitic photodynamic therapy
Débora P. Aureliano, José Angelo Lauletta Lindoso, Sandra Regina de Castro Soares, Cleusa Fumika Hirata Takakura, Thiago Martini Pereira, Martha Simões Ribeiro
Photodiagnosis and Photodynamic Therapy.2018; 23: 1. CrossRef
Grandiflorenic acid promotes death of promastigotes via apoptosis-like mechanism and affects amastigotes by increasing total iron bound capacity
Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Fernanda Tomiotto-Pellissier, Milena Menegazzo Miranda-Sapla, João Paulo Assolini, Amanda Cristina Machado Carloto, Priscila Goes Camargo de Carvalho, Ian Lucas Alves Cardoso, Andréa Name Colado
Phytomedicine.2018; 46: 11. CrossRef
In vitro Effects of Ketotifen and Cromolyn Sodium on Promastigote and Amastigotes of Leishmania major
Lima Asgharpour Sarouey, Khadijeh Khanaliha, Parvaneh Rahimi-Moghaddam, Samaneh Khorrami, Mohammad Saaid Dayer, Fatemeh Tabatabaie
Jundishapur Journal of Microbiology.2018;[Epub] CrossRef
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Marhiah C. Montoya, Damian J. Krysan, Danielle A. Garsin
mBio.2018;[Epub] CrossRef
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Álvaro Martín-Montes, Daniel Plano, Rubén Martín-Escolano, Verónica Alcolea, Marta Díaz, Silvia Pérez-Silanes, Socorro Espuelas, Esther Moreno, Clotilde Marín, Ramón Gutiérrez-Sánchez, Carmen Sanmartín, Manuel Sánchez-Moreno
Antimicrobial Agents and Chemotherapy.2017;[Epub] CrossRef
Evaluation of the Cytotoxicity Effect of Chaerophyllum Extract on Leishmania major and J774 Cell Line in Vitro
Parisa Ebrahimisadr, Hamidreza Majidiani, Farahnaz Bineshian, Farnoosh Jameie, Ezatollah Ghasemi, Fatemeh Ghaffarifar
Jundishapur Journal of Natural Pharmaceutical Products.2016;[Epub] CrossRef
Evaluation of the Cytotoxicity Effect of Chaerophyllum Extract on Leishmania major and J774 Cell Line in Vitro
Parisa Ebrahimisadr, Hamidreza Majidiani, Farahnaz Bineshian, Farnoosh Jameie, Ezatollah Ghasemi, Fatemeh Ghaffarifar
Jundishapur Journal of Natural Pharmaceutical Products.2016;[Epub] CrossRef

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Antileishmanial and Cytotoxic Effects of Essential Oil and Methanolic Extract of Myrtus communis L.: Hossein Mahmoudvand, Fatemeh Ezzatkhah, Fariba Sharififar, Iraj Sharifi, Ebrahim Saedi Dezaki; Korean J Parasitol 2015;53(1):21-27.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.21

Abstract
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Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were α-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P<0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC50 values for essential oil and methanolic extract was 8.4 and 28.9 μg/ml against promastigotes, respectively. These values were 11.6 and 40.8 μg/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC₅₀ values of 88.3 and 44.6 μg/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.

Citations

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Identification of novel Leishmania major antigens that elicit IgG2a response in resistant and susceptible mice: Mohammad Reza Mohammadi, Majid Zeinali, Sussan K. Ardestani, Amina Kariminia; Korean J Parasitol 2006;44(1):43-48.
Published online March 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.1.43

Abstract
PDF

Experimental murine models with high, intermediate and low levels of genetically based susceptibility to Leishmania major infection reproduce almost entire spectrum of clinical manifestations of the human disease. There are increasing non-comparative studies on immune responses against isolated antigens of L. major in different murine strains. The aim of the present study was to find out whether there is an antigen that can induce protective immune response in resistant and susceptible murine strains. To do that, crude antigenic extract of procyclic and metacyclic promastigotes of L. major was prepared and subjected to SDS-PAGE electrophoresis. Western-blotting was used to search for antigen(s) capable of raising high antibody level of IgG2a versus IgG1 in the sera of both infected resistant and susceptible strains. Two novel antigens from metacyclic promastigotes of L. major (140 and 152 kDa) were potentially able to induce specific dominant IgG2a responses in BALB/c and C57BL/6 mice. The 2 antigens also reacted with IgG antibody of cutaneous leishmaniasis patients. We confirm that 140 and 152 kDa proteins of L. major promastigotes are inducing IgG production in mice and humans.

Citations

Citations to this article as recorded by

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