Sarcocystosis was diagnosed worldwide by serodiagnostic tests utilising the whole parasite, for which the protozoa were maintained in vitro are more costly. In this study, antigenicity of Sarcocystis falcatula recombinant protein (rSfSAG4) was investigated towards the local communities of Pangkor and Tioman Islands and its seroprevalence was surveyed in these islands. A total of 348 human sera were tested using rSfSAG4 by Western blot and ELISA. High prevalence of sarcocystosis was observed in Tioman Island (80.6%) than in Pangkor Island (50.0%) by Western blot. In ELISA, the seroprevalence observed in Tioman Island was 45.9%, whereas in Pangkor Island 63.0%. In other parasitic infections, the prevalence was 34.0% by Western blot and 46.0% by ELISA. In healthy control group, 7% by Western blot and 8% by ELISA showed positivity to rSfSAG4. It is suggested SfSAG4 is a candidate antigen to measure seroprevalence of sarcocystosis.
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Sarcocystis infection in domestic and wild avian hosts: Inseparable flight partners Petras Prakas, Rafael Calero-Bernal, Jitender P. Dubey Veterinary Parasitology.2025; 335: 110413. CrossRef
Sarcocystis sp. infection was investigated in 20 necropsied captive wild mammals and 20 birds in 2 petting zoos in Malaysia. The gross post-mortem lesions in mammals showed marbling of the liver with uniform congestion of the intestine, and for birds, there was atrophy of the sternal muscles with hemorrhage and edema of the lungs in 2 birds. Naked eye examination was used for detection of macroscopic sarcocysts, and muscle squash for microscopic type. Only microscopically visible cysts were detected in 8 animals and species identification was not possible. Histological examination of the sections of infected skeletal muscles showed more than 5 sarcocysts in each specimen. No leukocytic infiltration was seen in affected organs. The shape of the cysts was elongated or circular, and the mean size reached 254 × 24.5 ?m and the thickness of the wall up to 2.5 ?m. Two stages were recognized in the cysts, the peripheral metrocytes and large numbers of crescent shaped merozoites. Out of 40 animals examined, 3 mammals and 5 birds were positive (20%). The infection rate was 15% and 25% in mammals and birds, respectively. Regarding the organs, the infection rate was 50% in the skeletal muscles followed by tongue and heart (37.5%), diaphragm (25%), and esophagus (12.5%). Further ultrastructural studies are required to identify the species of Sarcocystis that infect captive wild animals and their possible role in zoonosis.
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Gastrointestinal parasites of zoonotic importance observed in the wild, urban, and captive populations of non‐human primates in Malaysia Madinah Adrus, Ramlah Zainudin, Mariana Ahamad, Mohd‐Azlan Jayasilan, Mohd Tajuddin Abdullah Journal of Medical Primatology.2019; 48(1): 22. CrossRef
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Human and animal sarcocystosis in Malaysia: A review Baha Latif, Azdayanti Muslim Asian Pacific Journal of Tropical Biomedicine.2016; 6(11): 982. CrossRef
Neglected Tropical Diseases among Two Indigenous Subtribes in Peninsular Malaysia: Highlighting Differences and Co-Infection of Helminthiasis and Sarcocystosis Soo Ching Lee, Romano Ngui, Tiong Kai Tan, Roslan Muhammad Aidil, Yvonne Ai Lian Lim, David Joseph Diemert PLoS ONE.2014; 9(9): e107980. CrossRef
Initial Patient Cluster and First Positive Biopsy Findings in an Outbreak of Acute Muscular Sarcocystis-Like Infection in Travelers Returning from Tioman Island, Peninsular Malaysia, in 2011 Dennis Tappe, Karen Ernestus, Stephan Rauthe, Christoph Schoen, Matthias Frosch, Andreas Müller, August Stich Journal of Clinical Microbiology.2013; 51(2): 725. CrossRef
Zoonoses: a potential obstacle to the growing wildlife industry of Namibia Kudakwashe Magwedere, Maria Y. Hemberger, Louw C. Hoffman, Francis Dziva Infection Ecology & Epidemiology.2012;[Epub] CrossRef
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