The author reported previously on separation of the outer tegument of the spargana (plerocercoids of Spirometra mansoni) using high concentration of urea solution. To determine which layer of the tegument is separated by this method, an electron microscopic analysis has been processed in this study. It was confirmed that the basement layer of the tegument is separated from the parenchyme of the sparganum. In addition, the antigenicity of the separated outer tegument against the human sparganosis patient sera was evaluated. Numerous antigenic proteins, including 16 and 55 kDa proteins, were noticed in the separated tegument; however, there were no diagnostic 31/36 kDa molecules in this tegument. The molecules reactive with the patient sera in the tegument are to be characterized in future studies.
Neutrophils are important effector cells against protozoan extracellular parasite Entamoeba histolytica, which causes amoebic colitis and liver abscess in human beings. Apoptotic cell death of neutrophils is an important event in the resolution of inflammation and parasite's survival in vivo. This study was undertaken to investigate the ultrastructural aspects of apoptotic cells during neutrophil death triggered by Entamoeba histolytica. Isolated human neutrophils from the peripheral blood were incubated with or without live trophozoites of E. histolytica and examined by transmission electron microscopy (TEM). Neutrophils incubated with E. histolytica were observed to show apoptotic characteristics, such as compaction of the nuclear chromatin and swelling of the nuclear envelop. In contrast, neutrophils incubated in the absence of the amoeba had many protrusions of irregular cell surfaces and heterogenous nuclear chromatin. Therefore, it is suggested that Entamoeba-induced neutrophil apoptosis contribute to prevent unwanted tissue inflammation and damage in the amoeba-invaded lesions in vivo.
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Signaling Role of NADPH Oxidases in ROS-Dependent Host Cell Death Induced by Pathogenic Entamoeba histolytica Young Ah Lee, Seobo Sim, Kyeong Ah Kim, Myeong Heon Shin The Korean Journal of Parasitology.2022; 60(3): 155. CrossRef
The state of art of neutrophil extracellular traps in protozoan and helminthic infections César Díaz-Godínez, Julio C. Carrero Bioscience Reports.2019;[Epub] CrossRef
Entamoeba histolytica Trophozoites Induce a Rapid Non-classical NETosis Mechanism Independent of NOX2-Derived Reactive Oxygen Species and PAD4 Activity César Díaz-Godínez, Zayda Fonseca, Mario Néquiz, Juan P. Laclette, Carlos Rosales, Julio C. Carrero Frontiers in Cellular and Infection Microbiology.2018;[Epub] CrossRef
Entamoeba histolytica induces human neutrophils to form NETs J. Ventura‐Juarez, MR. Campos‐Esparza, J. Pacheco‐Yepez, J. A López‐Blanco, A. Adabache‐Ortíz, M. Silva‐Briano, R. Campos‐Rodríguez Parasite Immunology.2016; 38(8): 503. CrossRef
Modulatory mechanisms of enterocyte apoptosis by viral, bacterial and parasitic pathogens Andre G Buret, Amol Bhargava Critical Reviews in Microbiology.2014; 40(1): 1. CrossRef
Anoikis potential of Entameba histolytica secretory cysteine proteases: Evidence of contact independent host cell death Sudeep Kumar, Rajdeep Banerjee, Nilay Nandi, Abul Hasan Sardar, Pradeep Das Microbial Pathogenesis.2012; 52(1): 69. CrossRef
In vivo programmed cell death of Entamoeba histolytica trophozoites in a hamster model of amoebic liver abscess J. D. Villalba-Magdaleno, G. Perez-Ishiwara, J. Serrano-Luna, V. Tsutsumi, M. Shibayama Microbiology.2011; 157(5): 1489. CrossRef
Involvement of Src Family Tyrosine Kinase in Apoptosis of Human Neutrophils Induced by Protozoan ParasiteEntamoeba histolytica Seobo Sim, Jae-Ran Yu, Young Ah Lee, Myeong Heon Shin The Korean Journal of Parasitology.2010; 48(4): 285. CrossRef
Calpain-dependent calpastatin cleavage regulates caspase-3 activation during apoptosis of Jurkat T cells induced by Entamoeba histolytica Kyeong Ah Kim, Young Ah Lee, Myeong Heon Shin International Journal for Parasitology.2007; 37(11): 1209. CrossRef
Involvement of β2-integrin in ROS-mediated neutrophil apoptosis induced by Entamoeba histolytica Seobo Sim, Soon-Jung Park, Tai-Soon Yong, Kyung-Il Im, Myeong Heon Shin Microbes and Infection.2007; 9(11): 1368. CrossRef
Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase‐3 and reducing Mcl‐1 expression J. H. KANG, H. O. SONG, J. S. RYU, M. H. SHIN, J. M. KIM, Y. S. CHO, J. F. ALDERETE, M. H. AHN, D. Y. MIN Parasite Immunology.2006; 28(9): 439. CrossRef
Toxoplasma gondii Inhibits Apoptosis in Infected Cells by Caspase Inactivation and NF-κB Activation Ji-Young Kim, Myoung-Hee Ahn, Hye-Sun Jun, Jai-Won Jung, Jae-Sook Ryu, Duk-Young Min Yonsei Medical Journal.2006; 47(6): 862. CrossRef
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