Experimental meningoencephalitis by <i>Naegleria fowleri</i> in mice

, Ahn, Myoung Hee; , Im, Kyung Il

doi:1984.22.2.253

Korean J Parasito > Volume 22(2):1984 > Article

Original Article


Korean J Parasitol. 1984 Dec;22(2):253-258. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1984.22.2.253
Copyright © 1984 by The Korean Society for Parasitology


Experimental meningoencephalitis by Naegleria fowleri in mice
Myoung-Hee Ahn and Kyung-Il Im
Department of Parasitology, College of Medicine, Hanyang University, Korea.


Abstract
Experimentally, primary amoebic meningoencephalitis (PAM) is induced by Naegleria fowleri in mouse and development of PAM may be influenced by the strain, weight and sex of mouse, and inoculum size of N. fowleri trophozoite. In this paper, the effect of these factors on PAM development of mouse was studied. N. fowleri trophozoites, strain 0359, were introduced into mouse intranasally under secobarbital anesthesia (0.05 mg/g). 1. PAM was developed more frequently in BALB/c mouse than ICR mouse. 2. The survival time of mouse with PAM was influenced by the weight, that is, it was shorter in 15 g mouse than in the heavier groups. 3. No difference was observed on PAM development according to sex. 4. In case of inoculated amoeba, PAM incidence of 0.5 × 10⁴ was markedly decreased.

Figures


	Fig. 1 Cumulative death rate of mice with PAM due to Nagleria fowleri infection according to sex.


	Fig. 2 Cumulative death rate of mice with PAM due to Nagleria fowleri infection according to the weight.


	Fig. 3 Numerous trophozoites of Nagleria fowleri are observed with a number of eosinophils, neutrophils or macrophages in mouse brain. (×1,000)

Tables


	Table 1 Mortality of mice inoculated with Nagleria fowleri into nasal cavity


	Table 2 Survival time of mice with PAM due to Nagleria fowleri infection

References


1.	Apley J, Clarke SK, Roome AP, Sandry SA, Saygi G, Silk B, Warhurst DC. Primary amoebic meningoencephalitis in Britain. Br Med J 1970;1(5696):596–599.

2.	Butt CG. Primary amebic meningoencephalitis. N Engl J Med 1966;274(26):1473–1476.

3.	Butt CG, Baro C, Knorr RW. Naegleria (sp.) identified in amebic encephalitis. Am J Clin Pathol 1968;50(5):568–574.

4.	Callicott JH Jr. Amebic meningoencephalitis due to free-living amebas of the Hartmannella (Acanthamoeba)-Naegleria group. Am J Clin Pathol 1968;49(1):84–91.

5.	Carter RF. Description of a Naegleria sp. isolated from two cases of primary amoebic meningo-encephalitis, and of the experimental pathological changes induced by it. J Pathol 1970;100(4):217–244.

6.	Carter RF. Primary amoebic meningo-encephalitis. An appraisal of present knowledge. Trans R Soc Trop Med Hyg 1972;66(2):193–213.

7.	Culbertson CG, Ensminger PW, Overton WM. Hartmannella (acanthamoeba). Experimental chronic, granulomatous brain infections produced by new isolates of low virulence. Am J Clin Pathol 1966;46(3):305–314.

8.	Culbertson CG. The pathogenicity of soil amebas. Annu Rev Microbiol 1971;25:231–254.

9.	Darby CP, Conradi SE, Holbrook TW, Chatellier C. Primary amebic meningoencephalitis. Am J Dis Child 1979;133(10):1025–1027.

*10.*	Duma RJ, Rosenblum WI, McGehee RF, Jones MM, Nelson EC. Primary amoebic meningoencephalitis caused by Naegleria. Two new cases, response to amphotericin B, and a review. Ann Intern Med 1971;74(6):923–931.

*11.*	Fowler M, Carter RF. Acute pyogenic meningitis probably due to Acanthamoeba sp.: a preliminary report. Br Med J 1965;2(5464):740–742.

*12.*	Lee DK, Lee KT, Im KI. [Changes In The Pathogenicity Of Naegleria Fowleri By Several Brain Passage In Mice]. Korean J Parasitol 1983;21(2):234–240.

*13.*	Griffin JL. Temperature tolerance of pathogenic and nonpathogenic free-living amoebas. Science 1972;178(63):869–870.

*14.*	Haggerty RM, et al. Proc Helminthol Soc 1980;47:129–134.

*15.*	De Jonckheere J, van de Voorde H. Comparative study of six strains of Naegleria with special reference to nonpathogenic variants of Naegleria fowleri. J Protozool 1977;24(2):304–309.

*16.*	Markowitz SM, Martinez AJ, Duma RJ, Shiel FO. Myocarditis associated with primary amebic (Naegleria) meningoencephalitis. Am J Clin Pathol 1974;62(5):619–628.

*17.*	Martinez AJ, et al. Lab Inv 1973;29:121–123.

*18.*	Naginton J, Watson PG, Playfair TJ, McGill J, Jones BR, Steele AD. Amoebic infection of the eye. Lancet 1974;2(7896):1537–1540.

*19.*	Page FC. Taxonomic criteria for limax amoebae, with descriptions of 3 new species of Hartmannella and 3 of Vahlkampfia. J Protozool 1967;14(3):499–521.

*20.*	Patras D, Andujar JJ. Meningoencephalitis due to Hartmannella (Acanthamoeba). Am J Clin Pathol 1966;46(2):226–233.

*21.*	Phillips BP. Naegleria: another pathogenic ameba studies in germfree guinea pigs. Am J Trop Med Hyg 1974;23(5):850–855.

*22.*	Rothrock JF, Buchsbaum HW. Primary amebic meningoencephalitis. JAMA 1980;243(22):2329–2330.

*23.*	Stevens AR, Shulman ST, Lansen TA, Cichon MJ, Willaert E. Primary amoebic meningoencephalitis: a report of two cases and antibiotic and immunologic studies. J Infect Dis 1981;143(2):193–199.

*24.*	Thong YH, Ferrante A, Rowan-Kelly B, O'Keefe D. Immunization with live amoebae, amoebic lysate and culture supernatant in experimental Naegleria meningoencephalitis. Trans R Soc Trop Med Hyg 1980;74(5):570–576.

*25.*	Visvesvara GS, Jones DB, Robinson NM. Isolation, identification, and biological characterization of Acanthamoeba polyphaga from a human eye. Am J Trop Med Hyg 1975;24(5):784–790.

*26.*	Visvesvara GS, Healy GR. Disc electrophoretic patterns of esterase isoenzymes of Naegleria fowleri and N. gruberi. Trans R Soc Trop Med Hyg 1980;74(3):411–412.

*27.*	Wang SS, Feldman HA. Isolation of hartmannella species from human throats. N Engl J Med 1967;277(22):1174–1179.

*28.*	Willaert E, Stevens AR. Experimental pneumonitis induced by Naegleria fowleri in mice. Trans R Soc Trop Med Hyg 1980;74(6):779–783.

*29.*	Wong MM, Karr SL Jr, Balamuth WB. Experimental infections with pathogenic free-living amebae in laboratory primate hosts: I (A) A study on susceptibility to Naegleria fowleri. J Parasitol 1975;61(2):199–208.

*30.*	Wong MM, Karr SL Jr, Chow CK. Changes in the virulence of Naegleria fowleri maintained in vitro. J Parasitol 1977;63(5):872–878.