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A study on the lipids of Chinese liverfluke, Clonorchis sinensis
Soon-Hyung Lee,Chul Yong Song and Byong Ha Cho
Department of Parasitology, College of Medicine, Chung-Ang University, Korea.
Abstract
The present study was undertaken to observe the quality and quantity of lipids in the adult worms of Chinese liverfluke, Clonorchis sinensis.
Lipid extraction was done by the methods of Folch et a1. (l957) and Kenny (1952), and then the extracted lipid fractions of the worm were separated by thin layer chromatography. Those fractions were also subjected to perform the quantitative analyses of glycerides, cholesterols and phospholipids.
The results obtained were summarized as follows.
1. Total amount of glyceride was 37.56 mg per gram of worm tissue and the amount of monoglyceride was 8.34 mg per gm; diglyceride, 15.46 mg per gm; and triglyceride, 12.86 mg per gm.
2. Total amount of cholesterol was 3.30 mg per gm of worm tissue, and the esterified cholesterol (1.72 mg/gm) was a little more than that of free cholesterol (1.26 mg/gm).
3. The following 8 phospholipids were detected in the worm tissue of C. sinensis, i.e., lysophosphatidylcholine, phosphatidylcholine, phophatidylinositol, sphingomyelin, phosphatidylglycerol, phosphatidylserine, phosphatidylethanolamine and an unknown phospholipid.
Figures
Fig. 1 Thin layer chromatogram of phospholipid fractions of Clonorchis sinensis.
Table 2 Cholesterol contents in Clonorchis sinensis
Table 3 Phospholipid contents in Clonorchis sinensis
References
1.
Barrett J, Cain GD, Fairbairn D. Sterols in Ascaris lumbricoides (Nematoda), Macracanthorhynchus hirudinaceus and Moniliformis dubius (Acanthocephala), and Echinostoma revolutum (Trematoda). J Parasitol 1970;56(5):1004–1008.
2.
Beames CG Jr, Fisher FM Jr. A Study on the Neutral Lipids and Phospholipids of the Acanthocephala Macracanthorhynchus Hirudinaceus and Moniliformis Dubius. Comp Biochem Physiol 1964;13:401–412.
3.
Biezenski JJ. Federation Proceed 1964;23:503.
4.
Doviasova M, et al. Biochem J 1964;90:538–549.
5.
Fairbairn D, Wertheim G, Harpur RP, Schiller EL. Biochemistry of normal and irradiated strains of Hymenolepis diminuta. Exp Parasitol 1961;11:248–263.
6.
Folch J, et al. Biol Chem 1957;226:497–509.
7.
Goil MM. Physiological Studies on Trematodes--lipid Fractions in Gastrothylax Crumenifer. Parasitology 1964;54:81–85.
8.
Harvey SW, et al. Arch Biochem Biophys 1951;30:14–24.
9.
Kenny AP. The determination of cholesterol by the Liebermann-Burchard reaction. Biochem J 1952;52(4):611–619.
10.
Meyer F, Kimura S, Mueller JF. Lipid metabolism in the larval and adult forms of the tapeworm Spirometra mansonoides. J Biol Chem 1966;241(18):4224–4232.
11.
Meyer F, et al. Biochem Biophys Acta 1970;210:257–266.
12.
Moss GD. The excretory metabolism of the endoparasitic digenean Fasciola hepatica and its relationship to its respiratory metabolism. Parasitology 1970;60(1):1–19.
13.
Randle PJ, Garland PB, Hales CN, Newsholme EA. The glucose fatty-acid cycle. Its role in insulin sensitivity and the metabolic disturbances of diabetes mellitus. Lancet 1963;1(7285):785–789.
14.
Skipski VP, Peterson RF, Barclay M. Quantitative analysis of phospholipids by thin-layer chromatography. Biochem J 1964;90(2):374–378.
15.
Smith TM, Brooks TJ Jr. Lipid fractions in adult Schistosoma mansoni. Parasitology 1969;59(2):293–298.
16.
Smith TM, Lucia HL, Doughty BL, von Lichtenberg FC. The role of phospholipids in schistosome granulomas. J Infect Dis 1971;123(6):629–639.
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