Action of several chemicals on the parasites eggs and larvae in Korean Pickle(Kimchi)

, Kim, Chong Hwan; , Yoon, Tae Yeun

doi:1966.4.1.47

Korean J Parasito > Volume 4(1):1966 > Article

Original Article


Korean J Parasitol. 1966 Aug;4(1):47-51. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1966.4.1.47
Copyright © 1966 by The Korean Society for Parasitology


Action of several chemicals on the parasites eggs and larvae in Korean Pickle(Kimchi)
Chong-Hwan Kim and Tae-Yeun Yoon
Department of Parasitology, Yonsei University College of Medicine, Korea.



Abstract
In Korea where night soil used as fertilizer for crops and vegetables they may be contaminated simultaneously with infective stage of ascaris, hookworm and other helminthes. In this circumstance, the ascaris eggs and hookworm larvae may adhere to certain kinds of leafy vegetables(Chyu, 1957) and may remain viable even after pickling in brine or in other substances (Han and Ko, 1952; Soh, 1960). It has been shown that Heliogen(Thitasut, 1961) will kill the parasite eggs and larvae, and Sodium nitrite (Kozai, 1960; Kim and Soh 1964) and Thiabendazole (Kutsmi, 1964 and 1965; Kim et al., 1966) also had ovicidal action. The present study has been planned to study the ovicidal and larvicidal actions of several chemicals in Korean pickle juice(Kimchi) and the results are summarized as follows: 1. Heliogen solution(iodine 100 ppm) did not destroy the ascaris and hookworm eggs within 30 minutes at the temperature of 15-30℃, but the hookworm larvae were killed within 3 minutes under the same conditions. 2. Sod. nitrite which was added to pickle juice at the rate of 0.1% destroyed more than 90% of ascaris fresh eggs within 10 days and the embryonated eggs were destroyed within 2 days. Hookworm eggs were destroyed within 3 days and larvae were killed within 7 hours. 3. Thiabendazole which was added to pickle juice at the rate of 0.1-0.025% inhibited the infectivity of ascaris embryonated eggs within 24 hours and hookworm eggs were destroyed in the same concentrations of the reagent. 4. Hydrogen ion concentration of pickle juice which was mixed with Sodium nitrite (0.1%) was not changed for 10 days. 5. 1.5 mg of Sodium nitrite or Thiabendazole produced no toxicity in kidney and intestine of mice but slight pathological changes in the liver of the same animal.

Tables


	Table 1 Survival of the Eggs and Larvae of Parasites in Heliogen Solution(Iodine concentration : 100 ppm)


	Table 2 Survival of Ascaris lumbricoides Eggs in Pickle Juice Which were mixed with Sod. nitrite and Thiabendazole respectively


	Table 3 Hydrogen Ion Concentration of Pickle Juice Which were mixed with Sod. nitrite(final concentration, 0.1%) (Temp. (12±5℃))


	Table 4 A Survival of Fresh Eggs of Ancylostoma caninum in Pickle Juice were mixed with Sod. nitrite and Thiabendazole (Temp. (12±5℃))


	Table 4 B Survival of Filariform Iarvae of Ancylostoma caninum in Pickle Juice were mixed with Sod. nitrite(%) (Temp. (22±3℃))


	Table 5 Histo-pathological changes of mice which were given 1.5mg (0.5 mg, tid) of Sod. nitrite and Thiabendazole respectively

References


1.	Hunter GW, et al. J Parasit 1949;35:41.

2.	Soh CT. The effects of natural food-preservative substances on the development and survival of intestinal helminth eggs and larvae. I. Action on Ascaris lumbricoides eggs. Am J Trop Med Hyg 1960;9:1–7.

3.	Soh CT. The effects of natural food-preservative substances on the development and survival of intestinal helminth eggs and larvae. II. Action on Ancylostoma duodenale larvae. Am J Trop Med Hyg 1960;9:8–10.

4.	Soh CT, et al. Yonsei Medical Journal 1961;2:31–41.

5.	Thitasut P. Action of aqueous solutions of iodine on fresh vegetables and on the infective stages of some common intestinal nematodes. Am J Trop Med Hyg 1961;10:39–43.