Studies on the mineral contents of some trematodes

, Lee, Sang Whon

doi:1965.3.3.73

Korean J Parasito > Volume 3(3):1965 > Article

Original Article


Korean J Parasitol. 1965 Dec;3(3):73-85. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1965.3.3.73
Copyright © 1965 by The Korean Society for Parasitology


Studies on the mineral contents of some trematodes
Sang Whon Lee, M.D.
Department of Preventive Medicine, School of Medicine, Kyungpook National University, Taegu, Korea.



Abstract
The mineral contents of the adult Clonorchis sinensis from rabbits and human were measured, and its qualitative and semi-quantitative analyses were studied with 3.4 meter Ebert-Grating spectrograph, and its quantitative analyses were studied with Beckman DU spectrophotometer. The adult Clonorchis sinensis used in this study were divided into two groups, the first group was collected from the bile passage of the man (C. sinensis from man), the second group was collected from the bile passage of the rabbit(C. sinensis from rabbit). Simultaneously, spectrographic and photometric analyses were also performed on the adult worms of Paragonimus westermani. Furthermore, comparative spectrographic analyses of the trace elements were carried out on the C. sinensis from man, C. sinensis from rabbit, Fasciola hepatica, Eurytrema pancreaticum, and Paragonimus westermani, and the approximate contents of the trace elements of the above trematodes were compared with that of their host tissues and biles. The results obtained were as follows: 1. In the spectrographic analyses of C. sinensis from man, sodium, phosphorus, potassium, copper, calcium, aluminum, iron, and magnesium were detected predominantly, and zinc, titanium, silicone, lead, manganese, barium, cromium, molybdenum, and silver were detected as trace elements. 2. In the differences of level of the minerals in these two C. sinensis, copper level of C. sinensis from man was higher than that of C. sinensis from rabbit, while calcium level of the former worm was higher than that of the latter worm. The trace level of lead, molybdenum, and silver were acertained in the former, but latter were not detected. 3. The contents of the minerals showed the characteristic features in each trematodes: the mineral of flukes in each host were much more than that of the others; such as copper in C. sinensis from man, calcium in the C. sinensis from rabbit, and silicone in the P. westermani. The vanadium was detected in the F. hepatica, E. pancreaticum, and P. westermani, while the other flukes were not detected. 4. In the comparative spectrographic analyses of the trace elements among the trematodes and their host tissues and biles, the minerals which detected from flukes were also found in their tissues and biles of their host. But the mineral levels of C. sinensis from man, F. hepatica, E. pancreaticum, and P. westermani were higher than that of their host tissues and biles, except the C. sinensis from rabbit.

Figures


	Fig. 1 Slit width : 0.015 mm Excitation : 10 Amp. D.C


	Fig. 2 Spectrographic analysis of the ash from Clonorchis sinensis.


	Fig. 3


	Fig. 4


	Fig. 5 Spectrographic analysis of the ash from Paragonimus westermani.


	Fig. 6


	Fig. 7 Comparative spectrographic analyses of the ashes from trematodes, its host tissues and biles

Tables


	Table 1 Ebert-Grating Spectrographic analysis of the ash from Clonorchis sinensis which is collected from the bile passage of man


	Table 2 E.G.Spectrographic analysis of the ash from Clonorchis sinensis which is collected from the bile passage of rabbit^*


	Table 3 Comparison of results of E.G.Spectrographic analysis between C. sinensis from the bile passages of man and that of rabbit^*


	Table 4 E.G. Spectrographic analysis of the ash from Fasciola hepatica and Eurytrema pancreaticum which is collected from ox


	Table 5 E.G. Spectrographic analysis of the ash from Paragonimus westermani^*which is collected from the lung of dog


	Table 6 Mineral contents of the ash from Clonorchis sinensis which is collected from the bile passage of the man


	Table 7 Mineral contents of the ash form Clonorchis sinensis which is collected from the bile passage of the rabbit


	Table 8 Mineral contents of the ash form Paragonimus westermani which is collected from the lung of dog^*


	Table 9 Comparison of approximate contents of trace elements with E. G. Spectrographic analysis among trematodes, tissues of those habitats and biles

References


1.	Von Brand T. J Parasit 1939;25:329–342.

7.	Eden A, Green HH. Micro-determination of copper in biological material. Biochem J 1940;34(89):1202–1208.

8.	Haury VG. J Lab Clin Med 1937;23:1079–1084.

*10.*	Irving HM, et al. Analyst 1953;78:571–580.

*17.*	Ma L. Trace elements and polyphenol oxidase in Clonorchis sinensis. J Parasitol 1963;49:197–203.

*26.*	Stephonson W. Parasit 1947;38:140–144.

*28.*	Thompson JB. Ind Eng Chem Anual Ed 1944;16:646–648.