A Study on the chemotherapy in clonorchiasis. Report 1. An experimental study on chemotherapy with Dithiazanine iodide and Bithionol sulfoxide in clonorchiasis

, Kang, Suck Young; , Loh, In Kyu; , Chun, Yong Soo; , Lim, Do Soo

doi:1965.3.1.19

Korean J Parasito > Volume 3(1):1965 > Article

Original Article


Korean J Parasitol. 1965 Jun;3(1):19-30. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1965.3.1.19
Copyright © 1965 by The Korean Society for Parasitology


A Study on the chemotherapy in clonorchiasis. Report 1. An experimental study on chemotherapy with Dithiazanine iodide and Bithionol sulfoxide in clonorchiasis
Suck Young Kang,In Kyu Loh,Yong Soo Chun and Do Soo Lim
Department of Internal Medicine and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul, Korea.
Department of Parasitology, Medical General Laboratory, R.O.K. Army.
Department of Internal Medicine, Chunju Provincial Hospital, Korea.
Department of Internal Medicine, 1st Army Hospital, R.O.K. Army.


Abstract
Authors carried out experimental study on chemotherapy with oral administration of Dithiazanine iodide (D.I.) and Bithionol sulfoxide(B.S.) in rabbit clonorchiasis. And the following result was obtained. 1) In change of the E.P.G (eggs per gram feces) by D.I. administration, it was rather increased in early stage of the administration than prior to administration, and thereafter decreased gradually. 2) In the change of the C.S. worm body by D.I. administration, there was not only prominent change of supporting tissue but also the change of reproductive organ was found. 3) In considering the wormicidal effect of D.I. from detecting rate of survival worms, the effect was slight in group of 100 mg(80 mg/kg) per day dosage, but the effects were very excellent and almost complete by proper times of administration in groups of 200 mg (130 mg/kg) per day or higher dosage. 4) But the side effect and intoxication sign of D.I. were appearent in groups of 200 mg or higher dosage. 5) By B.S. administration, E.P.G. was decreased gradually. 6) In changes of the C.S. worm body by B.S. administration, prominent inhibitory chnnge was seen in egg formation ability. 7) Slight wormicidal effect of B.S. was observed in groups of 140 mg (100 mg/kg) per day or higher dosage. 8) Side effect and intoxication sign of B.S. were found little in groups of 140 mg or lesser dosage.

Figures


	Fig. 1 Central and mid part of control group (No. 1) Clonorchis sinensis. All organs is seen as normal (15×).


	Fig. 2 Magnified view of Fig. 1. Uterus (eggs), intestine and vitelline follicles are seen as normal (100×)


	Fig. 3 Clonorchis sinensis after treatment with dithiazanine iodide 300 mg (200 mg/kg) a day. Size was shrinked than that of Control (Fig. 1). Increase of number of eggs in uterus, degeneration of reproductive organ, poor stainability were shown (15×)


	Fig. 4 Magnified view of Fig. 3. Anterior part of Clonorchis sinensis, tegument, reticular tissue and intestine were degenerated and hyalinized (100×)


	Fig. 5 Magnified view of Fig. 3. Central part, ovary, seminal receptacle, testis et al were degenerated. Intestine was not clearly stained. Vacuolization of reticular tissue was seen (100×).


	Fig. 6 Clonorchis sinensis after treatment with dithiazanine iodide 100 mg (80 mg/kg) a day (No. 9). Decrease of number of eggs in uterus, degeneration of intestine and vitelline follicle were shown (100×).


	Fig. 7 Clonorchis sinensis after treatment with dithiazanine iodide 300 mg (200 mg/kg) a day (No. 103). Increased number of eggs in uterus, infiltration of eggs to the intestine, poor stainablility and hyalinization of ovary and seminal receptacle, degeneration of vitelline follicle were shown (100×).


	Fig. 8 Clonorchis sinensis after treatment with bithionol sulfate 80 mg (No. 36). In the uterus, rare eggs were seen. Degeneration of vitelline follicle was shown (100×).


	Fig. 9 Clonorchis sinensis after treatment with bithionol sulfate 80 mg (No. 28). In the uterus, a few eggs were seen (100×).


	Fig. 10 Clonorchis sinensis after treatment with bithionol sulfate 80 mg (No. 24). In the uterus, a degnerated eggs were seen (470×).


	Fig. 11 Clonorchis sinensis after treatment with bithionol sulfate 140 mg (No. 34). In the uterus, rare eggs were seen. Vitelline follicle-like materials were infiltrated in the uterus (100×).


	Fig. 12 Clonorchis sinensis after treatment with bithionol sulfate 200 mg (No. 26). In the uterus, a few eggs were seen. Vitelline follilces were degenerated and were shrinked (100×).

Tables


	Table 1 Experimental methods


	Table 2 Clonorchis sinensis infected vs nont-treated control


	Table 3 Therapeutic results from treated group with dithiazanine iodide 100 mg (80 mg/kg) a day


	Table 4 Therapeutic results from treated group with dithiazanine iodide 200 mg (130 mg/kg) a day


	Table 5 Therapeutic results from treated group with dithiazanine iodide 300 mg (200 mg/kg) a day


	Table 6 Therapeutic results from treated group with dithiazanine iodide 400 mg (260 mg/kg) a day


	Table 7 Metacercaria-cidal effect by treatment with bithionol sulfate in the test tube


	Table 8 Therapeutic results from mice treated with bithionol sulfate


	Table 9 Summary of the results of experiments


	Table 10 Size of recovered Clonorchis sinensis stained

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