Studies on the metabolism of C<sup>14</sup>-proline in some parasitic helminths

, Yoon, Joo Soo

doi:1964.2.3.159

Korean J Parasito > Volume 2(3):1964 > Article

Original Article


Korean J Parasitol. 1964 Dec;2(3):159-164. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1964.2.3.159
Copyright © 1964 by The Korean Society for Parasitology


Studies on the metabolism of C¹⁴-proline in some parasitic helminths
Joo Soo Yoon
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.


Abstract
A comparison of the absorption and incorporation of C¹⁴-proline into protein by the 7 kinds of helminth parasites is presented. The radioactivity of free amino acid fraction is greater than that of protein fraction in all the worms, and only a small amount of exogenous labeled proline is incorporated into tissue protein. In general, the pattern of C¹⁴-proline uptake and its incorporation into protein shows rapid linear increase during the period of 15 to 30 min and reaches the maximum at 60 min after incubation, and then the equilibrium state was maintained throughout further incubation.

Figures


	Fig. 1 The free amino acid fraction by the various helminth parasites. Total C¹⁴-activity/ medium:0.5 µC Weight of carrier proline: 400 µg. Temperature incubated: 37℃


	Fig. 2 The Incorporation of C¹⁴-proline into protein fraction by the various helminth parasites at 37℃


	Fig. 3 The uptake of C¹⁴-labeled proline and its incorporation into protein in Clonorchis sinensis after 15 min period of incubation at 37℃

Tables


	Table 1 The uptake and incorporation of C¹⁴-proline by various worm parasites at 37℃, 60 min.

References


1.	Chandler AC. Am J Hyg 1943;37:121–130.

2.	Daugherty JW. Exp Parasit 1952;1:331–338.

3.	Daugherty JW. Synthesis of amino nitrogen from ammonia in Hymenolepis diminuta. Proc Soc Exp Biol Med 1954;85(2):288–291.

4.	Daugherty JW. Intermediary protein metabolism in helminths. IV. The active absorption of methionine by the cestode, H. diminuta. Exp Parasitol 1957;6(1):60–67.

5.	Daugherty JW. The active absorption of certain metabolites by helminths. Am J Trop Med Hyg 1957;6(3):464–470.

6.	Daugherty JW, Foster WB. Comparative studies on amino acid absorption by cestodes. Exp Parasitol 1958;7(1):99–107.

7.	Foster WB, Daugherty JW. Establishment and distribution of Raillietina cesticillus in the fowl and comparative studies on amino acid metabolism of R. cesticillus and Hymenolepis diminuta. Exp Parasitol 1959;8:413–426.

8.	Hahn HJ. Korean J Intern Med 1963;6:377–382.

9.	Hahn HJ. Korean J Intern Med 1961;4:281–285.

*10.*	Hahn HJ. Korean J Intern Med 1962;5:357–360.

*11.*	Hankes LV, Stoner RD. Incorporation of DL-tyrosine-2-C-14 and DL-tryptophan-2-C-14 by encysted Trichinella spiralis larvae. Exp Parasitol 1958;7(1):92–98.

*12.*	Lee SH, Seo BS, Yoon JS. Paper Chromatographic Study On The Amino Acids Of Some Parasitic Helminths. Korean J Parasitol 1964;2(1):91–96.

*13.*	Ma L. Trace elements and polyphenol oxidase in Clonorchis sinensis. J Parasitol 1963;49:197–203.

*14.*	McCarthy CF, et al. J Clin Invest 1964;43:1518–1524.

*15.*	Read CP, Simmons JE Jr, Rothman AH. Permeation and membrane transport in animal parasites: amino acid permeation into tapeworms from elasmobranchs. J Parasitol 1960;46:33–41.

*16.*	Rothman AH, Fisher FM Jr. Permeation of Amino Acids in Moniliformis and Macracanthorhynchus (Acanthocephala). J Parasitol 1964;50:410–414.

*17.*	Seo, et al. Korean J Parasit 1964;2(2):22.

*18.*	Smyth JD. Ann NY Acad Sci 1959;77:102–125.

*19.*	Yoon JS, Lee SH, Seo BS. Autoradiographic Studies On Some Parasitic Helminth. Korean J Parasitol 1964;2(3):165–169.

*20.*	Zalokar M. Kinetics of amino acid uptake and protein synthesis in Neurospora. Biochim Biophys Acta 1961;46:423–432.