Studies on the experimental mode of infections of <i>Clonorchis sinensis</i>. II. Experimental infection of <i>Clonorchis sinensis</i> cercaria to fry of fresh water fishes

, Chun, Seh Kyu

doi:1964.2.3.137

Korean J Parasito > Volume 2(3):1964 > Article

Original Article


Korean J Parasitol. 1964 Dec;2(3):137-147. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1964.2.3.137
Copyright © 1964 by The Korean Society for Parasitology


Studies on the experimental mode of infections of Clonorchis sinensis. II. Experimental infection of Clonorchis sinensis cercaria to fry of fresh water fishes
Seh Kyu Chun
Pusan Fisheries College, Korea.


Abstract
Cercariae of Clonorchis sinensis were attempted to penetrate into various fry of fresh water fishes in order to examine the conditions of infection and the further development in the fish. The following conclusions were made. 1. Cercariae of C. sinensis penetrated into the muscle of the fry of Pseudorasbora parva were found to be able to develop. 2. Cercariae penetrated into the fry of Pseudorasbora parva migrated into the organs of the fry to kill their host. 3. From each dead individual of P. parva, more than 10 larvae of C. sinensis could be discovered. 4. Cercariae attached on the Cyprinus carpio, Carassius auratus and gold fish were almost killed before penetration into the body and even one or two cercariae penetrated were also killed as time passed. 5. Cercariae of C. sinensis infected on the fry of Cyprinus Carpio, Carassius auratus and gold fish could not survive more than 60 days. 6. Cercariae penetrated into Cyprinus carpio and Carassius auratus could not develop into complete metacercariae even after 3O days. 7. Cercariae of Clonorchis sinensis penetrated into the fry of Aplocheilus latipes were killed within 10 hours and the cercariae attached on the caudal fin were destroyed in 2 hours. 8. It was found that no cercariae of Clonorchis sinensis could penetrate into the body of the fry of Misgurnus anguillicaudatus.

Figures


	Fig. 1 Cercaria, attached an the surface of the p. parva (Clonorchis sinensis)

Plate I
Fig. 1. (A) A cercaria outflowed from siphon of a Parafossarulus manchouricus. (B) The cercariae of C. sinensis. ×50. (C) The tails of cercariae of C. sinensis. ×50.

Fig. 2. The cercariae of C. sinensis experimentally infected on the caudal fin of Pseudorasbora parva fry. (A). (B). (C). ×100.

Fig. 3. The number of the cercariae penetrated into the fry of Pseudorasbora parva increasing as time passes. (A). (B). (C). ×50.

Fig. 4. Development of metacercaria of C. sinsensis in the Pseudorasbora parva fry: (A) 24 hours after infection, ×270. (B) 3 days after infection, ×270. (C) 6 days after infection, ×270. (D) 15 days after infection, ×200. (E) 20 days after infection, ×270. (F) 30 days after infection, ×270.

Plate II
Fig. 5. Gradual changes of metacercaria of Clonorchis sinensis in the Cyprinus carpio fry: (A) 24 hours after infection ×300. (B) 3 days after infection, ×300. (C) 15 days after infection, ×300. (D) 30 days after infection, ×300.

Fig. 6. Gradual changes of metacercaria of C. sinensis. in the Carassius auratus fry, (gold fish). (A) 24hours after infection in the intestine of the gold fish. ×80. (B) An encystation of metacercaria taken out from the intestine of the gold fish. ×80. (C) A metacercaria degenerated in the muscle of the Carassius auratus, 15 days after infection. ×80.

Fig. 7. Cercariae of Clonoerchis sinensis in the internal organs of Cyprinus carpio, 24 hours after infection : (A) In the air bladder. ×200. (B) In the intestine. ×200.

Fig. 8. Experimental infection with cercariae of C. sinensis in the Aplocheilus latipes: (A) 20 minutes after infection in the body. ×50. (B) 60 minutes after infection in the caudal fin. All cercariae are destroyed. ×50.

Fig. 9. Experimental infection with cercariae of C. sinensis in the Misgurnus anguillicaudatus. (A). (B). No infection observed. ×50.

Tables


	Table 1 Result of experimental infection with larval Clonorchis sinensis to Pseudorasbora parva.


	Table 2 Result of experimental infection with larval Clonorchis sinensis to Cyprinus carpio.


	Table 3 Result of experimental infection with larval Clonorchis sinensis to Carassius auratus (Gold fish).


	Table 4 Result of experimental infection with larval Clonorchis sinensis to Carassius auratus.


	Table 5 Result of experimental infection with larval Clonorchis sinensis to Aplocheilus latipes.


	Table 6 Result of experimental infection with larval Clonorchis sinensis to Misgurnus anguillicaudatus.