Studies on the experimental mode of infections of <i>Clonorchis sinensis</i>. III. Studies on the wormicidal effect of external mucous substance of some fresh water fish on the larva of <i>Clonorchis sinensis</i>

, Chun, Seh Kyu

doi:1964.2.3.148

Korean J Parasito > Volume 2(3):1964 > Article

Original Article


Korean J Parasitol. 1964 Dec;2(3):148-156. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1964.2.3.148
Copyright © 1964 by The Korean Society for Parasitology


Studies on the experimental mode of infections of Clonorchis sinensis. III. Studies on the wormicidal effect of external mucous substance of some fresh water fish on the larva of Clonorchis sinensis
Seh Kyu Chun
Pusan Fisheries College, Korea.


Abstract
The author reported in the previous pager on his observation of sudden death of Clonorchis sinensis cercaria before penetration into the body of fry such as Cyprinus carpio and Carassius auratus. And in these studies, the reason behind the fact was investigated and it was found that the mucous substances on the surface of a certain fishes have a strong wormicidal effect to the larva of C. sinensis. 1. When cercariae of C. sinensis were immersed into the mucous substance of gold fish for three minutes 36 seconds to 4 minutes 20 seconds, into that of Aplocheilus latipes for 4 minutes 30 seconds to 5 minutes 20 seconds, into that of Cyprinus carpio for 5 minutes 15 seconds to 6 minutes l6 seconds, and into that of Carassius auratus for 5 minutes 20 seconds to 7 minutes 30 seconds, it was observed that they were all killed. 2. No wormicidal effect against C. sinensis could be observed with the mucous substance obtained from Pseudorasbora parva, Misgurnus anguillicaudatus and Ophicephalus argus. 3. No wormicidal effect against Metagonimus sp. cercaria is observed with the mucous substance obtained from Cyprinus carpio, Carassius auratus. 4. The wormicidal effect of the mucous substances obtained from gold fish was destroyed when heated over 60℃. 5. The movements of metacercaria of C. sinensis stopped after 10 minutes application of the mucous substance obtained from Cyprinus carpio and they were killed within 30 minutes. 6. When the adult worms collected from the bile duct of experimentally infected rabbits for 20 to 60 days, were immersed into mucous substance from Cyprinus carpio, they were killed between 25 minutes and 35 minutes. 7. No wormicidal effect could be observed with the mucous substance obtained from dead Cyprinus carpio, Carassius auratus, gold fish, when applied to the adult worms and larvae of C. sinensis.

Figures


	Fig. 1 Comparison of wormicidal effect of external mucous substance of the various kinds of fishes to the cercariae of C. sinensis.


	Fig. 2 Comparison of wormicidal effect of external mucous substance in the various kinds of fishes to the cerariae of C. sinensis.


	Fig. 3 Survival duration of the last individual cerearia in the external mucous of C. carpio depending in treated temperature.


	Fig. 4 Wormicidal effect of the external mucous substance of C. carpio on the excysted.


	Fig. 5 Wormicidal effect of the external mucous substance of C. carpio on the adult worms.

Plate I
Remarks:

Clonorchis sinensis cercaria in the following photograph was naturally obtaining from Parafossorulus manchouricus. Clonorchis sinensis cercariae are destroyed by the application of mucous substances obtained from Cyprinus carpio.

(A) The mucous substances of Cyprinus carpio were just applied.

(B) Severe spasms of cercarial body were observed after 3 minutes application.

(D) The tail was observed to bend S-form.

(E) The tail was observed to bend more increasely than S-form.

(F) The tail began to bend Z-form after 4'.

(G) The tail began to be destroyed.

(H) The tail began to be destroyed uneven form after 4'20''

(I) Oral sucker was ejected and the body began to be destroyed.

Plate II
Fig. 1. The cercariae of Clonorchis sinensis were destroyed by the external mucous of Carassius auratus: (A) 2 minutes 30 seconds after the application of nucous. (B) After 4 minutes. (C) After 5 minutes.

Fig. 2. The cercariae of Clonorchis sinensis were destroyed by the external mucous of Carassius auratus(Gold fish). (A) After 3 minutes. (B) After 4 minutes. (C) After 5 minutes.

Fig. 3. The excysted metacercariae were killed by the externalmucous of Cyprinus carpio. (A) After 10 minutes. (B) After 25 minutes.

Fig. 4. The adult worms were killed by the external mucous of Cyprinus carpio after 30 minutes.

Tables


	Table 1 Wormicidal effect of the external mucous substance of Cyprinus carpio on the cercaria of Clonorchis sinensis.


	Table 2 Wormicidal effect of the external mucous substance of Carassius auratus on the cercaria of Clonorchis sinensis.


	Table 3 Wormicidal effect of the external mucous substance of Goldfish (Carassius auratus) on the cercaria of Clonorchis sinensis.


	Table 4 Wormicidal effect of the external mucous substance of Aplocheilus latipes on the cercaria of Clonorchis sinensis.


	Table 5 Wormicidal effect of the external mucous substance of Anguilla japonica on the cercaria of Clonorchis sinensis.


	Table 6 Effect of the external mucous substance of Pseudorasbora parva on the cercaria of Clonorchis sinensis.


	Table 7 Effect of the external mucous substance of Misgurnus anguillicaudatus on the cercaria of Clonorchis sinensis.


	Table 8 Effect of the external mucous substance of Ophicephalus argus on the cercaria of Clonorchis sinensis.


	Table 9 Effect of the external mucous substance of Cyprinus carpio on the cercaria of Metagonimus sp.


	Table 10 Effect of the external mucous substance of Carassius auratus on the cercaria of Metagonimus sp.


	Table 11 Effect of the external mucous substance of Cyprinus carpio the cercariae of Clonorchis sinensis.


	Table 12 Effect of the external mucous substance of Cyprinus carpio on the excysted metacercariae of Clonorchis sinensis.


	Table 13 Effect of the external mucous substance of Cyprinus carpio on the adult worms of Clonorchis sinensis (60 days adult).


	Table 14 Effect of the external mucous substance of Cyprinus carpio on the adult worms of Clonorchis sinensis (20 days adult).

References


3.	Chun SK. Studies On The Experimental Mode Of Infections Of Clonorchis Sinensis: II. Experimental Infection Of Clonorchis Sinensis Cercaria To Fry Of Fresh Water Fishes. Korean J Parasitol 1964 Dec;2(3):137–147.