Distribution of the larvae of <i>Toxocara canis</i> and <i>Ascaris lumbricoides</i> in the tissues of mice

, Rim, Han Jong

doi:1963.1.1.37

Korean J Parasito > Volume 1(1):1963 > Article

Original Article


Korean J Parasitol. 1963 Jun;1(1):37-45. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1963.1.1.37
Copyright © 1963 by The Korean Society for Parasitology


Distribution of the larvae of Toxocara canis and Ascaris lumbricoides in the tissues of mice
Han Jong Rim
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.


Abstract
Mice were infected by feeding the embryonated eggs of Toxocara canis and Ascaris lumbricoides. Each mouse was killed daily for a week and then at several days interval after infection and the distribution of larvae in the various tissues of mice was investigated after the macerating the tissues and digesting with artificial gastric juice. It was confirmed that the migratory behaviour of larvae of T. canis and A. lumbricoides is referred to as the somatic and tracheal type of migration in the mice respectively. Toxocara larvae were found in the carcass on the third day after infection and in the brain after the sixth day of infection. From the thirty-fifth day to the seventy-sixth day after infection, Toxocara larvae were not found in the tissues of mice except in the carcass and brain and they did not develop further than the second-stage larvae. The size of Ascaris larvae, from the embryonated eggs was 0.228~0.271 mm length by 0.010~0.013 mm width and in the third day of infection the size of larvae was 0.271~0.343 mm length by 0.017~0.020 mm width. Between the fifth and tenth day after infection, lavrae molted twice in the lungs and grew to the fouth-stage larvae; 1.357~2.0 mm by 0.034~0.071 mm. These larvae migrated to the intestinal canal after the tenth day of infection and disappeared from the mouse after the twenty-fifth day of infection. No larvae were found in the carcass and brain. The inflammatory reactions in the tissues of infected mice were also observed.

Figures


	Fig. 1 A section of Toxocara canis larva found in the brain of mouse (M-25) at the seventy-sixth day after infection. (750×)


	Fig. 2 High magnification of Fig. 1 transverse section of T. canis larva. (1,200×)


	Fig. 3 The second-stage larva pressed from the egg of T. canis. (600×)


	Fig. 4 A section of Ascaris lumbricoides larva found in the liver of mouse (M-52) at the sixth day after infection. (750×)


	Fig. 5 High magnification of same serial section of Fig. 4, transverse section of A. lumbricoides larva. (1,800×)


	Fig. 6 A section of A. lumbricoides larva found in the lung of mouse (M-53) at the ninth day after infection. (160×)


	Fig. 7 The second-stage larva of T. canis recovered from the brain of mouse (M-14) at the fortieth day after infection. (120×)


	Fig. 8 The fourth-stage larva of A. lumbricoides recovered from the intestinal contents of mouse (M-39) at the thirteenth day after infection. (50×)


	Fig. 9 The head part of the fourth-stage larva of A. lumbricoides recovered from the intestinal contents of mouse (M-41) at the twenty-first day after infection. (600×)

Tables


	Table 1 Distribution of Toxocara canis larvae in the tissues of mice after inoculation of 500 eggs


	Table 2 Measurements(mm.) of Toxocara canis larvae recovered from the tissues of mice after inoculation of 500 eggs


	Table 3 Distribution of Ascaris lumbricoides larvae in the tissues of mice after inoculation of 1,000 eggs


	Table 4 Measurements(mm.) of Ascaris lumbricoides larvae recovered from the tissues of mice after inoculation of 1,000 eggs

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