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Original Article

Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea

Hyemi Lim1, Sang-Eun Lee2, Bong-Kwang Jung1, Min-Ki Kim1, Mi Youn Lee1, Ho-Woo Nam3, Jong-Gyun Shin4, Cheong-Ha Yun4, Han-Ik Cho4, Eun-Hee Shin1,5, Jong-Yil Chai1
The Korean Journal of Parasitology 2012;50(4):287-293.
Published online: November 26, 2012

1Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.

2Division of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Cheongwon 363-951, Korea.

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Corresponding author (cjy@snu.ac.kr)
• Received: September 21, 2012   • Revised: October 9, 2012   • Accepted: October 9, 2012

© 2012, Korean Society for Parasitology and Tropical Medicine

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  • Knowledge of the prevalence of human Toxoplasma gondii infection is required in the Republic of Korea. In this study, we surveyed the seroprevalence of T. gondii infection and analyzed the risk factors associated with seropositivity among residents in 2 administrative districts; Seoul and the island of Jeju-do, which have contrasting epidemiologic characteristics. Sera and blood collected from 2,150 residents (1,114 in Seoul and 1,036 in Jeju-do) were checked for IgG antibody titers using ELISA and for the T. gondii B1 gene using PCR. In addition, participants completed a questionnaire that solicited information on gender, age, occupation, eating habits, history of contact with animals, and travel abroad. The T. gondii B1 gene was not detected in all residents examined. However, ELISA showed 8.0% (89 of 1,114 sera) positive for IgG antibodies against T. gondii in Seoul and 11.3% (117 of 1,036 sera) in Jeju-do. In both districts, the positive rates were higher in males than in females, and those 40-79 years of age showed higher rates than other ages. In Seoul, residents older than 70 years of age showed the highest positive rate, 14.9%, whereas in Jeju-do the highest prevalence, 15.6%, was in those in their sixties. The higher seropositive rate in Jeju-do than in Seoul may be related to eating habits and occupations. The present results and a review of related literature are indicative of an increased seroprevalence of T. gondii in Korea in recent years.
  • Key words: Toxoplasma gondii, seroprevalence, B1 gene, resident, Seoul, Jeju-do, IgG, ELISA
Toxoplasma gondii, a coccidian protozoan, is distributed worldwide. This intracellular protozoan can infect essentially all nucleated eukaryotic cells of warm-blooded animals, including humans and vertebrate animals, and can cause clinical manifestations, such as lymphadenitis, encephalitis, retinochoroiditis, congenital infection, and neonatal mortality [1-3]. Transmission to humans occurs through ingestion of T. gondii bradyzoites or tachyzoites contained in undercooked meat of infected animals or ingestion of oocysts originating from the feces of infected cats [1-3]. Transplacental infection from mother-to-fetus is also well known [4]. In immunocompetent individuals, T. gondii infection is usually asymptomatic or causes only mild symptoms [1-3,5]. However, it can elicit severe clinical manifestations in immunocompromised conditions, including AIDS, transplantation, malignant disease, and pregnancy [5]. Congenital toxoplasmosis may cause stillbirth, abortion, or other serious damages to the fetus, such as meningoencephalitis and hydrocephalus [4,5].
In the Republic of Korea (hereafter referred to as Korea), the seroprevalence of toxoplasmosis has been studied by many researchers [6-23]. From 1960 until 1999, residents, children, and hospital patients in Korea usually showed relatively lower prevalences, ranging from 1.1-7.7%, compared to American and European locales [3,5,6-13]. However, from 2000-2009, the seroprevalence of toxoplasmosis showed a tendency of slight increase [14-20]. In particular, the prevalence on Jeju-do Island (hereafter referred to as Jeju-do) among an adult population was reported as 12.9% [14]. Thereafter, the reported seroprevalence in Korean residents have been, with the exception of one report [21], higher than this figure, ranging from 13.2-25.8% [22-24]. In this respect, it has been assumed that the seroprevalence of toxoplasmosis in Korea is presently increasing, presumably due to an increased consumption of domestic or imported pork, or other animal meat at risk of T. gondii infection [23].
The present study was conducted to determine the seroprevalence of T. gondii among people residing in 2 districts, Seoul and Jeju-do, which have contrasting epidemiologic characteristics related to the parasite life cycle and transmission. In addition, in order to assess the current status and trend of toxoplasmosis, the literature reporting the seroprevalence of toxoplasmosis among Korean people is briefly reviewed.
Subjects and sample collection
We collected sera and blood from 2,150 residents (1,114 in Seoul and 1,036 in Jeju-do; 12-95 years of age) from March to September 2011, during health check-up in the Eastern Seoul, Western Seoul, and Jeju-do branches of the Korea Association of Health Promotion (KAHP). The sera and blood were stored at -80℃ until analyzed. This study was approved under the regulations established by the Institutional Review Board Committees of Seoul National University Hospital (No. C-1101-064-348, 17 April 2011), and KAHP (No. 11-C-02, 22 March 2011). Informed consent was obtained from each individual.
Preparation of T. gondii lysate antigen
Tachyzoites of T. gondii (RH strain) were collected from the peritoneal cavity of 6 week old BALB/c mice that had been previously injected 3-4 days before. The tachyzoites were washed 3 times with PBS and purified by centrifugation over 40% Percoll (Sigma-Aldrich, St. Louis, Missouri, USA). The tachyzoites were sonicated 5 times on ice and centrifuged at 100,000 g for 1 hr. The supernatant was collected for use as the soluble antigen. The protein content was measured using a Nanodrop 2000 spectrometer (Thermo Scientific, Wilmington, Delaware, USA).
ELISA to measure IgG titers
ELISA was performed as previously described [11] with slight modifications. Briefly, 200 µl of T. gondii lysate antigen (TLA) (5 µg/ml) in 0.05 M carbonate-bicarbonate buffer (pH 9.6) was coated on each well of a 96-well microtiter plate (Costar, Cambridge, Massachusetts, USA), and the plate was incubated overnight at 4℃. After washing, each well was reacted with serum samples that were diluted 1:100 with 0.05% Tween 20. After incubation at 37℃ for 1 hr, a 1:10,000 dilution of horseradish peroxidase-conjugated goat anti-human IgG (Bethyl Laboratories, Montgomery, Texas, USA) was applied, and the plate was incubated at 37℃ for 1 hr. After several washes, freshly prepared o-phenylenediamine dihydrochloride (Sigma-Aldrich) was added, and the reaction was stopped by adding 8 N H2SO4. The IgG antibody titers were determined at the optical density of 490 nm.
PCR detection of T. gondii B1 gene in blood
The blood samples (n=1,036) from Jeju-do were examined for the presence of T. gondii B1 gene using PCR. The DNeasy blood and tissue kit (Qiagen, Hilden, Germany) was used for isolation of the genomic DNA of T. gondii. To amplify the 497 bp fragment of the gene, PCR was carried out using the following forward Toxo 497 (5'-AGCAAACAC-CGACGAACTCT-3') and reverse Toxo 497 (5'-CATGGTTTGCATTTTGTGG-3') primers. The smart 2X PCR Pre-mix (Solgent, Daejeon, Korea) was used with the following conditions: 94℃ for 5 min, followed by 35 cycles of 94℃ for 60 sec, 55℃ for 55 sec, and 72℃ for 60 sec. The conventional PCR products were analyzed by 1% agarose gel electrophoresis and stained with ethidium bromide.
Statistical analysis
To analyze the risk factors for toxoplasmosis, the chi-square test was applied. P<0.05 was considered statistically significant.
The serum IgG positive rate for T. gondii, as analyzed by ELISA, was 8.0% (89 positives among 1,114 sera) in Seoul and 11.3% (117 positives among 1,036 sera) in Jeju-do (Tables 1, 2). The average seropositive rate was 9.6% among all 2,150 residents (Table 1). The overall seroprevalence was a little higher in Jeju-do than in Seoul, although this difference was not statistically significant (P>0.05). By age group, however, the seroprevalence appeared to be consistently higher in Jeju-do than in Seoul through the age groups of 30-39, 40-49, 50-59, and 60-69 years (Fig. 1). In both districts, aged residents, particularly, those >40 years of age displayed markedly higher seroprevalences than younger residents (P<0.05) (Fig. 1). In Seoul, the highest positive rate, 14.9%, revealed in residents ≥70 years old, whereas in Jeju-do, the highest prevalence, 15.6% (Fig. 1) was evident in those in their 60s. There were also differences in the seropositive rate of male and female residents. Males usually showed higher seropositive rate than females in Seoul and Jeju-do (Table 1; Fig. 1). Particularly in Jeju-do, males showed higher seroprevalence than females with the exception of the age groups 20-29 year old and 30-39 year old age groups (Fig. 1). Overall, however, the sex difference of the seroprevalence was not statistically significant (P>0.05) (Table 1).
The results of the questionnaire study on epidemiologic factors related with T. gondii infection showed several positive findings, although statistical significance was not confirmed for all of the positive factors (Tables 2, 3). With regard to occupation, the 18.2% seropositive rate among Jeju-do residents who engaged in agriculture was markedly higher than the average value of 11.3%, although there was no statistical significance (P>0.05) (Table 2). Similarly, officers and housewives in Seoul and Jeju-do exhibited slightly lower seroprevalences (5.8-6.6% and 3.9-6.3%, respectively) than the average values (8.0% and 11.3%, respectively). Again, there was no statistical significance also (P>0.05) (Table 2).
The residents who preferred to eat a vegetarian diet were less likely to have infection with T. gondii (Table 3). The role of this factor was statistically significant particularly in Seoul (P<0.05). Jeju-do residents who drank bottled water were significantly less likely to get infections (P<0.05) (Table 3). Other factors, including the history of contact with animals and travel abroad, did not appear to be related with T. gondii infection both in Seoul and Jeju-do (Table 3).
The B1 gene of T. gondii was negative in all of the 1,036 blood samples collected from Jeju-do (samples from Seoul were not examined for the B1 gene), whereas the positive control containing T. gondii RH tachyzoites exhibited positive reaction band in PCR (data not shown).
The latex agglutination test (LAT) has been one of the most reliable diagnostic methods for toxoplasmosis for almost 4 decades [7,8,20]. However, its sensitivity has been estimated to be lower than that by ELISA both for serum and cerebrospinal fluid [11]. Therefore, nowadays in Korea, ELISA is more popularly used than LAT to estimate the seroprevalence of toxoplasmosis [12-19,21-24]. ELISA can accurately measure IgG, IgM, IgA, or IgE antibodies [25]. Detection of IgG antibody implies a chronic latent infection, whereas detection of IgM or IgA antibody is indicative of an acute infection [21,25]. However, IgM ELISA has a risk of false-positive results due to anti-nuclear antibodies or rheumatoid factor, or of detection of residual or persistent IgM months or even years after the primary infection [25]. Alternatively, IgG avidity test is performed to detect acute cases [25-27], and PCR for the T. gondii B1 gene is done to detect congenital or chronic clinical cases [28,29].
In the present study, IgG ELISA and PCR for the T. gondii B1 gene were carried out. All of the PCR-tested blood samples from Jeju-do revealed negative results. This may indicate that there were no acute clinical toxoplasmosis cases among the subjects examined. Unless a subject is suffering from acute infection, it is less likely for the T. gondii B1 gene to be detected from blood. Meanwhile, in IgG ELISA, 8.0% of residents in Seoul and 11.3% in Jeju-do revealed positive results. The IgG positive rates were higher in males than in females, and those from the forties to the seventies were more likely to be infected than younger individuals.
In the questionnaire, residents who preferred to eat a vegetarian diet and to drink bottled water were revealed to have a significantly lower (P<0.05) seroprevalence than those who ate meat and who consumed groundwater or well water. Those engaged in agriculture had a higher rate of seropositivity, whereas office workers and housewives tended to show lower seroprevalence, although there was no statistical significance (P<0.05) related with these occupation factors. It seems highly plausible that meat consumption is more closely related to infection with T. gondii than other possible factors in these 2 districts of Korea. Similarly, the higher seropositive rate in Jeju-do than in Seoul seems to be related to eating habits. It should be ruled out that the significantly lower seropositive rate among those who drank bottled water than those who consumed grounwater or well water might have been due to a lower risk of infection with T. gondii oocysts through bottled water.
Compared with previous studies conducted in various localities from 1960 until 2009, the results of the present study demonstrated fairly high seroprevalence of T. gondii infection among residents of Seoul and Jeju-do (Table 4; Fig. 2). For example, in Seoul, 1.1-7.0% seroprevalence was reported in 1982-1992 [7-11]. Thereafter, among children under 10 years of age in Seoul, a 1997 study revealed 7.7% seropositivity [13]. However, pregnant women in Seoul showed a prevalence of only 0.9% in 2005 [18]. Therefore, the prevalence of 8.0% in the present study is the highest seroprevalence of toxoplamosis ever reported from Seoul. With regard to Jeju-do, the seroprevalence was first studied in 1989 and 5.8% seroprevalence was reported from patients in a hospital [10]. Since then, several studies have reported the seroprevalence of toxoplasmosis in Jeju-do. A survey of high school students and adult hospital patients in Jeju-do revealed 5.5% and 12.9% seroprevalence, respectively [14]. In 2011 examination of outpatients of a general hospital in Jeju-do revealed a 13.2% IgG ELISA positive rate [22]. Compared with these reported seroprevalences, the 11.3% seroprevalence observed in the present study appears to indicate a sustanied, rather than decreased, level in Jeju-do.
In recent years, an increasing tendency in the seroprevalence of toxoplasmosis in Korea is evidently recognizable (Table 4; Fig. 2). Particularly, on islands in Gangwha-gun, the seroprevalence among residents were 15.8% in 2010 and 25.8% in 2011 which was the highest figure ever reported [23]. Subsequently, in Cheorwon-gun, the IgG ELISA seropositive rate was also high, at 17.0% [24]. The reason for this increasing tendency of seroprevalence is unclear, although an increase in the number of stray cats [30] and more popular consumption of meat, including pork, by Koreans [31] can be suggested as the possible reasons. Nevertheless, it should also be ruled out that the serologic techniques to detect IgG antibodies have been much improved and became more sensitive compared to those used in previous studies. This issue should be clarified in the near future.
The results of the present study strongly suggest that public health attention should be paid to toxoplasmosis prevalence in Korea.
This study was supported by a research grant from The Korea National Institute of Health, Ministry of Health and Welfare, the Republic of Korea (no. 800-20110036). We would like to thank staff of The Korea Association of Health Promotion for providing the sera and blood samples for this study. We have no special conflict of interest related with this study.
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Fig. 1
Age- and sex-specific seroprevalence of toxoplasmosis among residents in Seoul and Jeju-do assayed by IgG ELISA. The seroprevalence was generally higher in Jeju-do than in Seoul and in males than in females both in Seoul and Jeju-do, although these differences were not statistically significant (P>0.05).
kjp-50-287-g001.jpg
Fig. 2
Chronologic view of the seroprevalence of toxoplasmosis reported in Korea (1982-2012) based on data in Table 4 [7-24]. Each point represents % seropositive rate for each subjected group surveyed. Note that the seroprevalence in recent years (2010-2012) showed an increasing tendency.
kjp-50-287-g002.jpg
Table 1.
Seroprevalencea of Toxoplasma gondii infection among residents in Seoul and Jeju-do
Table 1.
District Male
 Female
 Total
No. examined No. positive (%) No. examined No. positive (%) No. examined No. positive (%)
Seoul 426 43 (10.1)b 688 46 (6.7)b 1,114 89 (8.0)d
Jeju-do 479 65 (13.6)c 557 52 (9.3)c 1,036 117 (11.3)d
Total 905 108 (11.9) 1,245 98 (7.9) 2,150 206 (9.6)

aSerum samples were collected and examined during March-September 2011.

b,c,dNo statistically significant differences were found between the paired 2 figures (P>0.05).

Table 2.
Seroprevalence of T. gondii infection according to the occupation of subjects in Seoul and Jeju-do
Table 2.
Type of occupation Seoul
 Jeju-do
 Total
No. examined No. ositive (%) No. examined No. positive (%) No. examined No. positive (%)
Agriculture 7 0 (0.0) 165 30 (18.2)a 172 30 (17.4)
Livestock 1 0 (0.0) 6 0 (0.0) 7 0 (0.0)
Fisheries 0 0 (0.0) 8 1 (12.5) 8 1 (12.5)
Officeb 207 12 (5.8) 126 8 (6.3) 333 20 (6.0)
Housewifec 106 7 (6.6) 76 3 (3.9) 182 10 (5.5)
Forestry 6 0 (0.0) 2 1 (50.0) 8 1 (12.5)
Mining 2 1 (50.0) 2 0 (0.0) 4 1 (25.0)
Services 7 1 (7.1) 35 3 (8.6) 42 4 (9.5)
Miscellaneous 778 68 (8.7) 616 71 (11.5) 1,394 139 (10.0)
Total 1,114 89 (8.0) 1,036 117 (11.3) 2,150 206 (9.6)

aResidents who are engaged in agriculture in Jeju-do showed higher seroprevalence than the average value, although the difference was not statistically significant (P>0.05).

b,cOffice workers and housewives in Seoul or Jeju-do showed lower seroprevalence than the average value; however, the difference was not statistically significant (P>0.05).

Table 3.
Relationship of epidemiologic factors and serologic results of T. gondii infection among residents in Seoul and Jeju-do
Table 3.
Epidemiologic factor Seoul
 Jeju-do
 Total
No. seropositive (Ta) No. sero-negative No. seropositive (Ta) No. sero-negative No. seropositive (Ta) No. sero-negative
Vegetarian diet 11b (21) 255 12 (16) 136 23b (40) 391
No 78b (67) 770 105 (100) 783 183b (166) 1,553
Using bottled water 49 (47) 551 44b (56) 457 93 (105) 1,008
No 40 (41) 474 73b (60) 462 113 (101) 936
Cooked food intake 80 (81) 942 83 (87) 688 163 (172) 1,630
No 9 (7) 83 34 (29) 231 43 (34) 314
Contact with animals 15 (12) 144 28 (21) 166 43 (34) 310
No 74 (76) 881 89 (95) 753 163 (172) 1,634
History of travel abroad 24 (28) 333 50 (54) 430 74 (80) 763
No 65 (60) 692 67 (62) 489 132 (126) 1,181

aTheoretical values for the observed ones (no. of seropositive cases).

bStatistical significance was noted (P<0.05) between these 2 variables.

Table 4.
Comparison of Toxoplasma gondii seropositive rates in Korea reported by different authors
Table 4.
Authors (year) Subjects Surveyed area No. positive/ No. examined Seropositive rate (%) Assay method Reference
Soh et al. (1960) Residents Korea 21/373 5.8 Skin test [6]
Choi et al. (1982) Patients in a university hospital Seoul 18/421 4.3 LAa [7]
Choi et al. (1983) Mental patients Seoul 11/573 1.9 LA [8]
Yoo and Choe (1986) Children ( < 15 years) Seoul 7/120 5.8 Hemag-glutination [9]
Choi et al. (1989) Patients in a hospital Seoul 19/1,019 1.9 LA [10]
Asthma patients Seoul 11/1,030 1.1 LA
Patients in a hospital Jeju-do 45/780 5.8 LA
Choi et al. (1992) Neurologic patients Seoul 76/2,016 3.8 LA [11]
Neurologic patients Seoul 142/2,016 7.0 ELISA
Ryu et al. (1996) Pregnant women Gyeonggi-do 39/899 4.3 ELISA [12]
High school students Gyeonggi-do 4/218 1.8 ELISA
Kook et al. (1999) Under 10-year children Seoul 42/542 7.7 LA [13]
Yang et al. (2000) Students Jeju-do 250/4,570 5.5 ELISA [14]
Adults Jeju-do 61/474 12.9 ELISA
Hong et al. (2000) High school students Jeju-do 250/4,320 5.5 ELISA [15]
Lee et al. (2000) Residents Okcheon-gun 74/1,109 6.9 ELISA [16]
Bae et al. (2001) Teachers of childbearing age Jeju-do 12/314 3.8 LA/ELISA [17]
Song et al. (2005) Pregnant women Seoul 51/5,725 0.9 ELISA [18]
Han et al. (2008) Pregnant women Daejeon 13/351 3.7 ELISA/LA [19]
Shin et al. (2009) Patients in a university hospital Daejeon 85/1,265 6.6 LA [20]
84/1,265 6.7 ELISA
Ko et al. (2011) Pregnant women Daejeon 19/787 2.4 ELISA [21]
Hong et al. (2011) Residents Jeju-do 309/2,348 13.2 ELISA [22]
Yang et al. (2012) Residents (2010) Gyeonggi-do 195/1,232 15.8 ELISA [23]
Residents (2011) Gyeonggi-do 335/1,296 25.8 ELISA
Ahn et al. (2012) Residents Gangwon-do 282/1,661 17.0 ELISA [24]
Lim et al. (2012)b Residents Seoul 89/1,114 8.0 ELISA -
Residents Jeju-do 117/1,036 11.3 ELISA -

aLA; latex agglutination test.

bPresent study.

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Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
Korean J Parasitol. 2012;50(4):287-293.   Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.287
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Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
Korean J Parasitol. 2012;50(4):287-293.   Published online November 26, 2012
DOI: https://doi.org/10.3347/kjp.2012.50.4.287
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Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
Image Image
Fig. 1 Age- and sex-specific seroprevalence of toxoplasmosis among residents in Seoul and Jeju-do assayed by IgG ELISA. The seroprevalence was generally higher in Jeju-do than in Seoul and in males than in females both in Seoul and Jeju-do, although these differences were not statistically significant (P>0.05).
Fig. 2 Chronologic view of the seroprevalence of toxoplasmosis reported in Korea (1982-2012) based on data in Table 4 [7-24]. Each point represents % seropositive rate for each subjected group surveyed. Note that the seroprevalence in recent years (2010-2012) showed an increasing tendency.

Fig. 1

Fig. 2

Serologic Survey of Toxoplasmosis in Seoul and Jeju-do, and a Brief Review of Its Seroprevalence in Korea
District Male
Female
Total
No. examined No. positive (%) No. examined No. positive (%) No. examined No. positive (%) Seoul 426 43 (10.1)b 688 46 (6.7)b 1,114 89 (8.0)d Jeju-do 479 65 (13.6)c 557 52 (9.3)c 1,036 117 (11.3)d Total 905 108 (11.9) 1,245 98 (7.9) 2,150 206 (9.6) Type of occupation Seoul
Jeju-do
Total
No. examined No. ositive (%) No. examined No. positive (%) No. examined No. positive (%) Agriculture 7 0 (0.0) 165 30 (18.2)a 172 30 (17.4) Livestock 1 0 (0.0) 6 0 (0.0) 7 0 (0.0) Fisheries 0 0 (0.0) 8 1 (12.5) 8 1 (12.5) Officeb 207 12 (5.8) 126 8 (6.3) 333 20 (6.0) Housewifec 106 7 (6.6) 76 3 (3.9) 182 10 (5.5) Forestry 6 0 (0.0) 2 1 (50.0) 8 1 (12.5) Mining 2 1 (50.0) 2 0 (0.0) 4 1 (25.0) Services 7 1 (7.1) 35 3 (8.6) 42 4 (9.5) Miscellaneous 778 68 (8.7) 616 71 (11.5) 1,394 139 (10.0) Total 1,114 89 (8.0) 1,036 117 (11.3) 2,150 206 (9.6) Epidemiologic factor Seoul
Jeju-do
Total
No. seropositive (Ta) No. sero-negative No. seropositive (Ta) No. sero-negative No. seropositive (Ta) No. sero-negative Vegetarian diet 11b (21) 255 12 (16) 136 23b (40) 391 No 78b (67) 770 105 (100) 783 183b (166) 1,553 Using bottled water 49 (47) 551 44b (56) 457 93 (105) 1,008 No 40 (41) 474 73b (60) 462 113 (101) 936 Cooked food intake 80 (81) 942 83 (87) 688 163 (172) 1,630 No 9 (7) 83 34 (29) 231 43 (34) 314 Contact with animals 15 (12) 144 28 (21) 166 43 (34) 310 No 74 (76) 881 89 (95) 753 163 (172) 1,634 History of travel abroad 24 (28) 333 50 (54) 430 74 (80) 763 No 65 (60) 692 67 (62) 489 132 (126) 1,181 Authors (year) Subjects Surveyed area No. positive/ No. examined Seropositive rate (%) Assay method Reference Soh et al. (1960) Residents Korea 21/373 5.8 Skin test [6] Choi et al. (1982) Patients in a university hospital Seoul 18/421 4.3 LAa [7] Choi et al. (1983) Mental patients Seoul 11/573 1.9 LA [8] Yoo and Choe (1986) Children ( < 15 years) Seoul 7/120 5.8 Hemag-glutination [9] Choi et al. (1989) Patients in a hospital Seoul 19/1,019 1.9 LA [10] Asthma patients Seoul 11/1,030 1.1 LA Patients in a hospital Jeju-do 45/780 5.8 LA Choi et al. (1992) Neurologic patients Seoul 76/2,016 3.8 LA [11] Neurologic patients Seoul 142/2,016 7.0 ELISA Ryu et al. (1996) Pregnant women Gyeonggi-do 39/899 4.3 ELISA [12] High school students Gyeonggi-do 4/218 1.8 ELISA Kook et al. (1999) Under 10-year children Seoul 42/542 7.7 LA [13] Yang et al. (2000) Students Jeju-do 250/4,570 5.5 ELISA [14] Adults Jeju-do 61/474 12.9 ELISA Hong et al. (2000) High school students Jeju-do 250/4,320 5.5 ELISA [15] Lee et al. (2000) Residents Okcheon-gun 74/1,109 6.9 ELISA [16] Bae et al. (2001) Teachers of childbearing age Jeju-do 12/314 3.8 LA/ELISA [17] Song et al. (2005) Pregnant women Seoul 51/5,725 0.9 ELISA [18] Han et al. (2008) Pregnant women Daejeon 13/351 3.7 ELISA/LA [19] Shin et al. (2009) Patients in a university hospital Daejeon 85/1,265 6.6 LA [20] 84/1,265 6.7 ELISA Ko et al. (2011) Pregnant women Daejeon 19/787 2.4 ELISA [21] Hong et al. (2011) Residents Jeju-do 309/2,348 13.2 ELISA [22] Yang et al. (2012) Residents (2010) Gyeonggi-do 195/1,232 15.8 ELISA [23] Residents (2011) Gyeonggi-do 335/1,296 25.8 ELISA Ahn et al. (2012) Residents Gangwon-do 282/1,661 17.0 ELISA [24] Lim et al. (2012)b Residents Seoul 89/1,114 8.0 ELISA - Residents Jeju-do 117/1,036 11.3 ELISA -
Table 1. Seroprevalencea of Toxoplasma gondii infection among residents in Seoul and Jeju-do

Serum samples were collected and examined during March-September 2011.

b,c,dNo statistically significant differences were found between the paired 2 figures (P>0.05).

Table 2. Seroprevalence of T. gondii infection according to the occupation of subjects in Seoul and Jeju-do

Residents who are engaged in agriculture in Jeju-do showed higher seroprevalence than the average value, although the difference was not statistically significant (P>0.05).

b,cOffice workers and housewives in Seoul or Jeju-do showed lower seroprevalence than the average value; however, the difference was not statistically significant (P>0.05).

Table 3. Relationship of epidemiologic factors and serologic results of T. gondii infection among residents in Seoul and Jeju-do

Theoretical values for the observed ones (no. of seropositive cases).

Statistical significance was noted (P<0.05) between these 2 variables.

Table 4. Comparison of Toxoplasma gondii seropositive rates in Korea reported by different authors

LA; latex agglutination test.

Present study.

Table 1.

Table 2.

Table 3.

Table 4.

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