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Original Article

Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba

Eun-Kyung Moon1, Yeonchul Hong1, Dong-Il Chung1, Youn-Kyoung Goo1, Hyun-Hee Kong2
The Korean Journal of Parasitology 2014;52(2):131-135.
Published online: April 18, 2014

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Corresponding author (hhkong@dau.ac.kr)
• Received: January 28, 2014   • Revised: March 3, 2014   • Accepted: March 3, 2014

© 2014, Korean Society for Parasitology and Tropical Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba
Korean J Parasitol. 2014;52(2):131-135.   Published online April 18, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.2.131
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Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba
Korean J Parasitol. 2014;52(2):131-135.   Published online April 18, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.2.131
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Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba
Image Image Image Image
Fig. 1 Transfection of Acanthamoeba with siRNA against cellulose synthase. Cellulose synthase (CS) was highly expressed during encystation (A-), but was down-regulated in CS-siRNA transfected cells (A-). This inhibition by CS-siRNA was found to reduce encystation ratios (>50% inhibition of mature cyst formation) (B). The experiments were repeated 3 times and the average values are presented with error bars representing standard deviations. **Means were significantly different by the Student's t-test (P<0.01).
Fig. 2 Cyst wall formation as detected by calcofluor white staining. (A) Cellulose was not present in trophozoites by calcofluor white staining. (B) At day 3 after the induction of encystation, young cysts and mature cysts (arrows) were observed. (C) The majority of cellulose synthase siRNA transfected cells were young or immature cysts.
Fig. 3 Cyst wall formation as detected by scanning electron microscopy. (A) Acanthamoeba trophozoites were transformed into mature cysts with 2 cyst walls (the ectocyst and endocyst). (B) In contrast to normal cells, cellulose synthase siRNA transfected cells did not form 2 cyst walls and remained as young cysts or died.
Fig. 4 The expression pattern of xylose isomerase. Xylose isomerase was expressed high in Acanthamoeba during encystation (). After transfection with cellulose synthase siRNA, the expression pattern of xylose isomerase was unchanged (). Accordingly, the down-regulation of cellulose synthase had no effects on the expression of xylose isomerase in Acanthamoeba.

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Down-Regulation of Cellulose Synthase Inhibits the Formation of Endocysts in Acanthamoeba
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