Infection Status of Hospitalized Diarrheal Patients with Gastrointestinal Protozoa, Bacteria, and Viruses in the Republic of Korea

Hyeng-Il Cheun; Shin-Hyeong Cho; Jin-Hee Lee; Yi-Young Lim; Ji-Hye Jeon; Jae-Ran Yu; Tong-Soo Kim; Won-Ja Lee; Seung-Hak Cho; Deog-Yong Lee; Mi-Seon Park; Hye-Sook Jeong; Doo-Sung Chen; Yeong-Mi Ji; Mi-Hwa Kwon

doi:10.3347/kjp.2010.48.2.113

Korean J Parasito > Volume 48(2):2010 > Article

Cheun, Cho, Lee, Lim, Jeon, Yu, Kim, Lee, Cho, Lee, Park, Jeong, Chen, Ji, and Kwon: Infection Status of Hospitalized Diarrheal Patients with Gastrointestinal Protozoa, Bacteria, and Viruses in the Republic of Korea

Original Article

The Korean Journal of Parasitology 2010; 48(2): 113-120.

Published online: June 17, 2010

DOI: https://doi.org/10.3347/kjp.2010.48.2.113

Infection Status of Hospitalized Diarrheal Patients with Gastrointestinal Protozoa, Bacteria, and Viruses in the Republic of Korea

Hyeng-Il Cheun^1,^†, Shin-Hyeong Cho^1,^†, Jin-Hee Lee², Yi-Young Lim¹, Ji-Hye Jeon¹, Jae-Ran Yu³, Tong-Soo Kim⁴, Won-Ja Lee¹, Seung-Hak Cho⁵, Deog-Yong Lee⁵, Mi-Seon Park⁵, Hye-Sook Jeong⁶, Doo-Sung Chen⁶, Yeong-Mi Ji⁶, Mi-Hwa Kwon⁷

¹Department of Malaria and Parasitic Diseases, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul, Korea.

²Department of AIDS, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul, Korea.

³Department of Environmental and Tropical Medicine, Konkuk University School of Medicine, Seoul, Korea.

⁴Department of Parasitology and Inha Research Institute for Medical Sciences, Inha University College of Medicine, Incheon, Korea.

⁵Department of Enteric Bacterial Infections, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul, Korea.

⁶Department of Enteric and Hepatitis Viruses, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul, Korea.

⁷Department of Influenza and Respiratory Viruses, Korea National Institute of Health, Korea Centers for Disease Control and Prevention, Seoul, Korea.

Corresponding author (ilcheun7@korea.kr)

Equal contributor:

†These authors equally contributed as the first author.

Received January 26, 2010 Revised April 15, 2010 Accepted April 21, 2010

Abstract

To understand protozoan, viral, and bacterial infections in diarrheal patients, we analyzed positivity and mixed-infection status with 3 protozoans, 4 viruses, and 10 bacteria in hospitalized diarrheal patients during 2004-2006 in the Republic of Korea. A total of 76,652 stool samples were collected from 96 hospitals across the nation. The positivity for protozoa, viruses, and bacteria was 129, 1,759, and 1,797 per 10,000 persons, respectively. Especially, Cryptosporidium parvum was highly mixed-infected with rotavirus among pediatric diarrheal patients (29.5 per 100 C. parvum positive cases), and Entamoeba histolytica was mixed-infected with Clostridium perfringens (10.3 per 100 E. histolytica positive cases) in protozoan-diarrheal patients. Those infected with rotavirus and C. perfringens constituted relatively high proportions among mixed infection cases from January to April. The positivity for rotavirus among viral infection for those aged ≤ 5 years was significantly higher, while C. perfringens among bacterial infection was higher for ≥ 50 years. The information for association of viral and bacterial infections with enteropathogenic protozoa in diarrheal patients may contribute to improvement of care for diarrhea as well as development of control strategies for diarrheal diseases in Korea.

Key words: Cryptosporidium parvum, Entamoeba histolytica, Clostridium perfringens, rotavirus, protozoa, bacteria, virus, mixed infection, hospitalization

INTRODUCTION

Acute diarrhea is one of the most common diseases worldwide, and causes approximately 2.5 million children death annually [1-3]. In the Republic of Korea (= Korea), the mortality rate in children younger than 5 years was 0.3% [4]. The protozoan parasite, including Cryptosporidium parvum, Giardia lamblia, and Entamoeba histolytica, have been under constant surveillance in developed countries, including USA, UK, and Japan due to their potential for causing water- and food-borne diarrheal outbreaks [5-7]. Also water- and food-borne viruses have been implicated with acute gastrointestinal diseases in humans, and these enteric viruses have occasionally been identified as the etiologic agents of waterborne disease outbreaks [8-11]. Rotavirus has been shown to be responsible for 25% to 46% of diarrhea episodes among hospitalized children < 5 years old in Korea, 2002-2004 [12].

The Korea National Institute of Health (KNIH) has surveyed pathogenic agents for hospitalized diarrheal patients every year from 2003 across the nation. This surveillance program examined 10 species of enteropathogenic bacteria, including Salmonella spp., Shigella spp., enterotoxigenic Escherichia coli, Vibrio parahaemolyticus, Yersinia enterocolitica, Staphylococcus aureus, Clostridium perfringens, Campylobacter jejuni, Listeria monocytogenes, and Bacillus cereus, and 4 species of viruses, including rotavirus, adenovirus, astrovirus, and norovirus in the stools of around 30,000 patients each year. The examination of major water- and food-borne protozoa (C. parvum, G. lamblia, and E. histolytica) causing gastrointestinal diseases was added to the program in 2004.

Global studies have identified and analyzed the pathogenic bacteria and viruses and protozoa that cause diarrhea, but there have been few studies in Korea. Furthermore, there is especially no study of mixed infections of viruses and bacteria in relation to protozoan infections in diarrheal patients. In this study, we evaluated the infections of acute gastrointestinal protozoa, bacteria, and viruses in hospitalized patients who had a major symptom of diarrhea. This study is the first to evaluate the infections of parasitic protozoa and their mixed infections with acute gastrointestinal pathogenic bacteria and viruses in patients who were hospitalized with a major symptom of diarrhea across the nation of Korea.

MATERIALS AND METHODS

Surveyed areas and stool samples

The survey was carried out as a part of the national program for control of diarrheal diseases from 2004 to 2006. The prevalence study was planned and implemented by the Department of Malaria and Parasitic Diseases, KNIH (Korea National Institute of Health). RIHE (Research Institute of Health and Environment) in 16 cities and provinces collected stools from 76,652 diarrheal patients who were hospitalized in 96 hospitals (Fig. 1). The diarrheal patients in this study were defined as those having diarrhea 3 or more times per day in watery or loose form accompanied by vomiting and abdominal pain. One gram or 1 loop of each stool sample was tested.

Protozoa

Cysts or oocysts of C. parvum, G. lamblia, and E. histolytica in the stool samples were identified with an enzyme immunoassay kit (Ridascreen, Darmstadt, Germany). Briefly, approximately 1 g of stool sample was suspended in 3.5 ml of distilled water and then filtered through moistened gauze. The filtrated liquid was centrifuged at 1,660 g for 3 min at 4℃. The supernatant was discarded and the pellet was tested according to the manufacturer's protocol. The absorbance was measured at 450 nm. Negative and positive controls were determined at absorbance values lower than 0.2 and higher then 0.8, respectively.

Bacteria

To identify bacteria (Salmonella spp., Shigella spp., enterotoxigenic E. coli (EPEC, ETEC, EAEC, STEC, EIEC), V. parahaemolyticus, Y. enterocolitica), a loop of each stool sample was directly inoculated into 3 ml of Luria Burtani (LB; Sparks, Maryland, USA) broth and incubated overnight at 37℃. The enriched broth culture was centrifuged at 23,000 g for 1 min. The pellet was heated to 100℃ for 10 min and spun down. A total of 5 µl of the supernatant was used in PCR. To detect pathogen-specific target gene, PCR was performed using the primers listed in Table 1. In brief, PCR was carried out in 50 µl with 2 U of DNA Taq polymerase (Takara, Tokyo, Japan) in a thermal cycler (PTC-100, MJ Research, Watertown, Massachusetts, USA). To further isolate, 8 different selective agar plates were used; MacConkey agar (BD) for E. coli, Salmonella, and Shigella spp, thiosulfatecitrate-bile salts-sucrose (TCBS, Oxoid, Basingstoke, Hampshire, UK) agar for Vibrio spp., mannitol-salt agar (MSA, BD) for S. aureus (toxins A, B, C, D, and E), tryptose-sulfite-cycloserine (TSC, Oxoid) for C. perfringens (alpha toxin, enterotoxin, or nontoxin), blood-free selective agar base (CCDA, BD) for C. jejuni, Listeria selective agar (LSA, Oxoid) for L. monocytogenes, cefsulodin-irgasan-novobiocin (CIN, Oxoid) for Y. enterocolitica, and mannitol-egg yolk-polymixin (MYP, Oxoid) for B. cereus (nemolysin BL-enterotoxin, nonhemolytic enterotoxin). One well-isolated colony from each bacteria culture was inoculated into 5 ml of 0.85% NaCl medium (pH 5.5 to 7.0). A humid atmosphere was used, and the API kit (Biomerieux, Marcy l'Etoile, France) was used as directed by the manufacturer to identify the organisms. The detection of Salmonella spp., pathogenic E. coli, C. perfringens, S. aureus, and B. cereus was considered positive regardless of the toxin in results.

Virus

Rotavirus, adenovirus, and astrovirus were analyzed using a VIro-Capture kit (Bioincell, Houston, Texas, USA), VIro-Capture kit (Bioincell), and IDEIA kit (DAKO, Glostrup, Denmark), respectively, according to the manufacturer's protocol. Norovirus was identified by reverse-transcription PCR [11].

Statistical analysis

Categorical variables were compared using the chi-square test. Logistic regression analysis was performed to examine factors that were associated with protozoa, viruses, and bacteria. The multi-variable models were fit adjusting for the variables (gender, age group, and area). Statistical significance (P < 0.05) was defined at the 95% confidence interval. SAS software (version 9.1) was used for all statistical analyses.

RESULTS

Positivity for protozoa, viruses, and bacteria

We examined 76,652 stool samples from diarrheal patients whose chief complaint was diarrhea. Positivity of our investigated 3 protozoa, 4 viruses, and 10 bacteria was 28,237 diarrheal patients (3,684 per 10,000 individuals), and their positivity was found to be 129 (95% confidence interval [CI], 121-137), 1,759 (95% CI, 1,732-1,785), and 1,797 (95% CI, 1,769-1,824) per 10,000 persons, respectively; it was lower for protozoa than for viruses and bacteria (P < 0.0001). Males showed a higher prevalence rate than females (55%, 33,166 persons) (P < 0.0001), but that was not different according to gender for parasites (P = 0.2334), viruses (P = 0.2206), and bacteria (P = 0.1198). We divided the age groups of diarrheal patients into ≤ 5 year, 6-9 year, 10-49 year, and ≥ 50 year groups. The highest number of diarrheal patients was ≤ 5 year group, 13,674 of 76,652 (4,240 per 10,000 individuals). The positivity for protozoa was significantly high in ≤ 5 year group compared to 6-9 year group (P = 0.0115). The positivity for viruses for those aged ≤ 5 years (P < 0.0001) was significantly high, while that for bacteria was low (P < 0.0001). The area distribution did not affect the positive rate for protozoan infections (P = 0.0601), but the adjusted odds ratio (AOR) (1.5, P < 0.0001) for viruses and for bacteria (1.1, P < 0.0001) was relatively high in provincial areas (Table 2).

Age-related positivity

Table 3 lists the positivity for protozoa, viruses, and bacteria among diarrheal patients by age group. Protozoan and viral infections were evident in ≤ 5 year group. The number of protozoan infections was 854 of 24,240 individuals (350 per 10,000 individuals), and 25.1% of C. parvum, 55.0% of G. lamblia, and 20.0% of E. histolytica positive rates were observed. The positivity for C. parvum was significantly higher at ≤ 5 years than at 10-49 years (P < 0.0001) and ≥ 50 year (P = 0.0033). The positivity for G. lamblia was higher than that for C. parvum and E. histolytica across all age groups (P < 0.0001). The positivity for norovirus, rotavirus, and adenovirus was low in old individuals, while that for astrovirus was high in those aged ≥ 50 year than in younger age groups (6-9 years and 10-49 years). In the case of bacteria, the positivity for Salmonella spp. was significantly high at 6-9 years compared to other age groups (P < 0.0001). The positivity for Shigella spp. was high in old age groups, but there was no significant difference (P = 0.7108). The positivity for V. parahaemolyticus, C. jejuni, and E. coli was highest at 10-49 years, and that of C. perfringens was highest at ≥ 50 years. The positivity for B. cereus was high across all ages (P = 0.0057), while that of S. aureus was significantly high at ≤ 5 years compared to other age groups (P < 0.0001). No significant difference was observed for L. monocytogenes and Y. enterocolitica due to their low positivity in each age group. The positivity for S. aureus was the highest in those aged ≤ 5 years, while that of C. perfringens was highest in other age groups.

Mixed-infections

The probability of mixed infection with viruse or bacteria among protozoan-infected cases (n = 987) was investigated (Table 4). A total of 535 patients of protozoan diarrhea was mixed-infected with viruses (311, 58.1%) or bacteria (224, 41.9%), respectively. Mixed infections with viruses were prominent in C. parvum diarrheal patients, and opposite results were observed in G. lamblia and E. histolytica infections. These 3 kinds of protozoa were mainly mixed-infected with rotavirus and C. perfringens. The positivity (probability) of mixed infection with C. parvum and rotavirus was the highest, 29.5 (95% CI, 23.9-35.1) per 100 C. parvum-infected persons, while that of mixed infection with E. histolytica and C. perfringens was 10.3 (95% CI, 6.0-14.6) per 100 E. histolytica-infected cases.

Seasonal prevalence

In Korea, cold winter months are from November to March, dry and mild spring months are from April to May, hot and moist summer months with sudden showers are from June to August, and dry and cool autumn months are September and October. The seasonal trends of mixed infections with protozoa and bacteria or viruses were analyzed (Fig. 2). The proportion of mixed infections was relatively lower in G. lamblia infection than in C. parvum or E. histolytica infection. The seasonal trend from January to April was higher with rotavirus and C. perfringens than the other pathogens.

DISCUSSION

This is the first systematic study in which the distributions of various enteropathogens and mixed infections related to diarrhea are described in Korea. Our findings highlight the importance of diarrheal diseases associated with protozoan infections. The parasitic protozoa might infect the intestinal mucosa and elicit transient or chronic diarrhea, which should be considered as one of the most important opportunistic gastrointestinal pathogens. C. parvum and G. lamblia might constitute the principal candidates for endemic transmission due mainly to their ubiquitous presence in drinking water and food contents, and partly to their high resistance to relevant environmental factors and chemical disinfecting procedures [13,14]. C. parvum was highly mixed-infected with rotavirus that often infects pediatric hospitals, which has a high infection rate amongst those aged ≤ 5 years, whose immune systems may be weaker than normal [15,16]. In developing countries, rotavirus infections in the gastrointestinal tract constituted a major cause of childhood death, especially in children younger than 5 years old. It has been responsible for approximately half a million deaths per year [9,10,17,18]. In the present study, C. parvum infection should be considered among viral mixed infections, which is important to consider in therapies applied to hospitalized children patients.

In Korea, most diagnoses and treatments for diarrheal patients have ignored protozoan infections because most clinicians focused their diagnosis on the pathogenic viruses and bacteria. In this situation, it is possible that the unknown pathogens accounting for outbreaks of over 12% of food-borne diseases contain manifold protozoa [19]. Even though the infection rate of gastrointestinal protozoa is not so high (1.0%), gastrointestinal infections with protozoan parasites should not be overlooked.

C. perfringens, which is a spore-forming bacterial indicator of fecal contamination and an important food-borne diarrheal causative agent, was found to be highly mixed-infected with protozoa. The infection rate for C. perfringens was the highest in those aged ≥ 50 years. C. perfringens was found in 7.6% of 662 stool samples from diarrheal patients, whereas infection rates of Salmonella spp., S. aureus, and Y. enterocolitica were 5.4%, 3.0%, and 0.2%, respectively [20]. Most pediatricians prescribe antibiotics in less than 20% of patients with acute gastroenteritis infection, while most physicians routinely prescribe antibiotics in Korea [21].

One question arising from this study is whether mixed infection with protozoa is more likely to induce serious diarrhea. As usual, single infection caused more severe symptoms compared to mixed infections in a patient. It is also depending on the strength of the pathogenic strain as well as the infectious species involved [22-25]. In most cases, detailed information about clinical symptoms and the infectious density of diarrheal patients was obscured in this study. Rotavirus and C. perfringens were, however, highly mixed-infected with enteropathogenic protozoa in diarrheal patients. Identification of etiologic agents of acute gastrointestinal infections has important implications for their management and prevention [26]. A better understanding of the determinants of diarrheal medical care and drugs prescribed is clearly required to address the burden of acute gastrointestinal infection in Korea. The novel information obtained in the present study regarding infection and mixed-infection status of enteropathogenic bacteria and viruses with protozoa in diarrheal patients will help in further isolation and treatment of diarrheal diseases.

ACKNOWLEDGEMENTS

We express our gratitude to our colleagues in the RIHEs of the 16 cities and provinces for their collection of stools from diarrheal patients. They also performed laboratory examinations to detect 3 protozoan parasites, 10 bacteria, and 4 viruses. This work was supported by a grant from the National Institute of Health (NIH-091-4800-4851-300), National Research and Development Program, Ministry of Health and Welfare, the Republic of Korea.

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Fig. 1

Sampling areas (16 Regional Institutes of Health and Environment, Korea) and protocol of the present study. Numbers within the circle represent the number of hospitals subjected in each institute.

Fig. 2

Seasonal patterns of mixed infections with protozoa. Noro, norovirus; Rota, rotavirus; Adeno, enteric adenovirus; Astro, astrovirus; Clos, Clostridium perfringens (alpha toxin, enterotoxin, or nontoxin); Staph, Staphylococcus aureus (toxins A, B, C, D, and E); E. coli, pathogenic E. coli (STEC, EAEC, EPEC, ETEC, and EIEC); Bacil, Bacillus cereus (hemolysin BL-enterotoxin, nonhemolytic enterotoxin); Shi, Shigella flexneri.

Table 1.

Primers used in this study

Pathogen	Target gene	Sequence (5′-3′)	Size of PCR product (bp)	GenBank No. or reference
Salmonella spp.	Invasion-associated locus (inv)	ATTAATTATGGAAGCGCTCGCATT	247	U84286
Salmonella spp.	Invasion-associated locus (inv)	GTAATGAGATCCATCAAATTAGCG
Shigella spp.	Invasion-associated locus (ial)	GTTGCGCTTGATGGGTGGGGTATC	356	L25276
Shigella spp.	Invasion-associated locus (ial)	GAAATGTCCATCAAACCCCACTC
Escherichia coli STEC	Shiga toxin 1 (stx1)	CGTACGGGGATGCAGATAAATCGC	210	AB048231
	Shiga toxin 1 (stx1)	CAGTCATTACATAAGAACGCCCAC
	Shiga toxin 2 (stx2)	GTTCTGCGTTTTGTCACTGTCAC	326	AB048835
	Shiga toxin 2 (stx2)	GTCGCCAGTTATCTGACATTCTGG
Escherichia coli EAEC	Heat-stable enterotoxin (east1)	ATGCCATCAACACAGTATATCCG	119	AB042002
Escherichia coli EAEC	Heat-stable enterotoxin (east1)	TCAGGTCGCGAGTGACGGCTTT
Escherichia coli EPEC	Attaching and effacing (eaeA)	ATGCTGGCATTTGGTCAGGTCGG	233	AF319597
Escherichia coli EPEC	Attaching and effacing (eaeA)	TGACTCATGCCAGCCGCTCATGCG
Escherichia coli ETEC	Heat-labile toxin (lt)	GATCACGCGAGAGGAACACAAACC	366	X83966
	Heat-labile toxin (lt)	ATCTGTAACCATCCTCTGCCGGAG
	Heat-stable toxin (st)	CTTTCCCCTCTTTTAGTCAGTC	167	M35586
	Heat-stable toxin (st)	CACAGGCAGGATTACAACAAAGT
Escherichia coli EIEC	Invasion-associated locus (ial)	GTTGCGCTTGATGGGTGGGGTATC	356	In this study
Escherichia coli EIEC	Invasion-associated locus (ial)	GAAATGTCCATCAAACCCCACTC
Vibrio parahaemolyticus	Thermostable direct hemolysin (tdh)	CTTCCATCTGTCCCTTTTCCTGCC	217	S76724
Vibrio parahaemolyticus	Thermostable direct hemolysin (tdh)	ATGTTCACAGTCATGTAGGATGTC
Yersinia enterocolitica	Attachment invasion locus (ail)	TTATCAATTGCGTCTGTTAATGTG	449	M29945
Yersinia enterocolitica	Attachment invasion locus (ail)	GACTTTGGAGTATTCATATGAAGC
Norovirus	Capsid region (GI)	CTGCCCGAATTYGTAAATGAT GAT	314	Kim et al. 2005
	Capsid region (GI)	CCAACCCARCCATTRTACATYTG
	Capsid region (GII)	GGGAGGGCGATCGCAATCT	313	Kim et al. 2005
	Capsid region (GII)	CCRCCIGCATRICCRTTRTACAT

Table 2.

Positivity for protozoa, viruses, and bacteria in hospitalized diarrheal patients in Korea, 2004-2006

	Distribution of study population	Protozoa				Virus				Bacteria
	Number (%)	No. of positives	Positivity (95% CI)	AOR (95% CI)	P-value	No. of positives	Positivity (95% CI)	AOR (95% CI)	P-value	No. of positives	Positivity (95% CI)	AOR (95% CI)	P-value
Total	76,652	987	129			13,479	1,759			13,771	1,797
			(121-137)				(1,732-1,785)				(1,769-1,8244)
GENDER (n = 60,230)
Male	33,166	382	196	Reference	-	5,746	1,733	Reference	-	5,908	1,781	Reference	-
	(55)		(174-217)				(1,692-1,773)				(1,740-1,823)
Female	27,064	284	115	0.9	0.2334	4,614	1,705	1.0	0.2206	4,795	1,772	1.0	0.1198
	(45)		(104-127)	(0.8-1.1)			(1,660-1,750)	(1.0-1.1)			(1,726-1,817)	(0.9-1.0)
AGE (year) (n = 62,867)
Mean age: 23 (SE : 27.7) years
Median age: 5 (IQR : 1-49) years
≤5	32,278	442	137	Reference	-	8,622	2,671	Reference	-	4,610	1,428	Reference	-
	(51.3)		(124-150)				(2,623-2,719)				(1,390-1,466)
6-9	2,979	30	101	0.8	0.0115	433	1,454	0.5	< 0.0001	645	2,165	1.6	< 0.0001
	(4.7)		(65-137)	(0.8-1.1)			(1,327-1,580)	(0.4-0.5)			(2,017-2,313)	(1.5-1.8)
10-49	12,216	130	106	0.8	0.2123	930	761	0.2	< 0.0001	2,336	1,912	1.4	< 0.0001
	(19.4)		(88-125)	(0.6-0.9)			(714-808)	(0.2-0.3)			(1,843-1,982)	(1.4-1.5)
≥50	15,394	201	131	1.0	0.6195	1,010	656	0.2	< 0.0001	3,196	2,076	1.6	< 0.0001
	(24.5)		(113-149)	(0.8-1.1)			(617-695)	(0.2-0.2)			(2,012-2,140)	(1.5-1.7)
Area (n = 76,652)
Province	38,022	537	141	Reference	-	8,056	1,426	Reference	-	7,277	1,708	Reference	-
	(49.6)		(129-153)				(1,391-1,461)				(1,670-1,746)
City	38,630	450	117	0.9	0.0601	5,423	2,085	1.5	< 0.0001	6,494	1,884	1.1	< 0.0001
	(50.4)		(106-127)	(0.8-1.0)			(2,045-2,126)	(1.4-1.5)			(1,845-1,923)	(1.1-1.2)

Positivity is per 10,000 individuals; CI, confidence interval; AOR, adjusted odds ratio; SE, standard error; IQR, interquantile range.

Table 3.

Distribution of protozoan, viral, and bacterial positivity by age of hospitalized diarrheal patients in Korea, 2004-2006

Pathogen	Age group (years)
	≤5		6-9		10-49		≥50
	No. of positives	Relative positivity (95% CI)	No. of positives	Relative positivity (95% CI)	No. of positives	Relative positivity (95% CI)	No. of positives	Relative positivity (95% CI)
Cryp	146	45 (38-53)	9	30 (11-50)	20	16 (9-24)	40	26 (18-34)
Gia	240	74 (65-84)	17	57 (30-84)	89	73 (58-88)	124	81 (66-95)
Enta	92	29 (23-34)	6	20 (4-36)	22	18 (11-26)	49	32 (23-41)
Noro	2,232	692 (664-719)	165	554 (472-636)	467	382 (348-416)	433	281 (255-307)
Rota	5,814	1801 (1,759-1,843)	233	782 (686-879)	345	282 (253-312)	371	241 (217-265)
Adeno	637	197 (182-213)	27	91 (57-125)	67	55 (42-68)	73	47 (37-58)
Astro	403	125 (113-137)	27	91 (57-125)	83	68 (53-83)	173	112 (96-129)
Sal	297	92 (82-102)	79	265 (208-323)	153	125 (106-145)	167	109 (92-125)
Shi	6	2 (0-3)	7	24 (6-41)	31	25 (17-34)	49	32 (23-41)
Vib	17	5 (3-8)	11	37 (15-59)	203	166 (144-189)	92	60 (48-72)
E. coli	1,277	396 (374-417)	171	574 (491-658)	720	589 (548-631)	747	485 (451-519)
Cam	31	10 (6-13)	10	34 (13-54)	57	47 (35-59)	19	12 (7-18)
Clos	1,411	437 (415-459)	291	977 (870-1,084)	880	720 (675-766)	1,612	1047 (999-1,096)
Staph	1,610	499 (475-523)	107	359 (292-426)	325	266 (238-295)	536	348 (319-377)
Bacil	391	121 (109-133)	41	138 (96-180)	184	151 (129-172)	266	173 (152-193)
List	1	0.3 (0-0.9)	0	0	3	3 (0-5)	3	2 (0-4)
Yer	14	4 (2-7)	4	13 (0-27)	6	5 (1-9)	7	5 (1-8)

Positivity is per 10,000 individuals; CI, confidence interval.

The number of cases was counted to each mixed-infected case for individual who has simultaneously several pathogens.

Cryp, Cryptosporidium parvum; Gia, Giardia lamblia; Enta, Entamoeba histolytica; Sal, Sallmonela spp. (Typhimurium and Enteritidis); Shi, Shigella spp.; Vib, Vibrio parahaemolyticus; E. coli, Pathogenic Escherichia coli (STEC, EAEC, EPEC, ETEC, and EIEC); Cam, Campylobacter jejuni; Clo, Clostridium perfringens (alpa toxin, Enterotoxin, or non-toxin); Staph, Staphylococcus aureus (toxin A, B, C, D, and E); Bacil, Bacillus cereus (Hemolysin BL-enterotoxin, Non-hemolytic enterotoxin); List, Listeria monocytogenes; Yer, Yersinia enterocolotica; Noro, Norovirus; Rota, Rotavirus (Group A); Adeno, Enteric adenovirus; Astro, Astrovirus.

Table 4.

Mixed-infections with viruses and bacteria among individuals infected with protozoans in hospitalized diarrheal patients in Korea, 2004-2006

Pathogen	Total No. of protozoa		Cryptosporidium parvum		Giardia lamblia		Entamoeba histolytica
Pathogen	No. of positives	Positivity/100 infected individuals (95% CI)	No. of positives	Positivity/100 infected individuals (95% CI)	No. of positives	Positivity/100 infected individuals (95% CI)	No. of positives	Positivity/100 infected individuals (95% CI)
Noro	45	4.6 (3.3-5.9)	12	4.7 (2.1-7.3)	28	4.7 (3.0-6.4)	8	4.1 (1.3-6.9)
Rota	165	16.7 (14.4-19.0)	75	29.5 (23.9-35.1)	90	15.2 (12.3-18.1)	20	10.3 (6.0-14.6)
Adeno	26	2.6 (1.6-3.6)	12	4.7 (2.1-7.3)	8	1.3 (0.4-2.3)	12	6.2 (2.8-9.6)
Astro	75	7.6 (5.9-9.3)	6	2.4 (0.5-4.2)	58	9.8 (7.4-12.2)	18	9.3 (5.2-13.4)
Sal	11	1.1 (0.5-1.8)	0	0.0	8	1.3 (0.4-2.3)	3	1.5 (0-3.3)
Shi	3	0.3 (0-0.6)	0	0.0	3	0.5 (0-1.1)	0	0.0
Vib	5	0.5 (0.06-1.0)	0	0.0	4	0.7 (0-1.3)	1	0.5 (0-1.5)
E. coli	51	5.2 (3,8-6.5)	13	5,1 (2.4-7.8)	25	4.2 (2.6-5.8)	14	7.2 (3.6-10.0)
Cam	1	0.1 (0-0.3)	0	0.0	1	0.2 (0-0.5)	0	0.0
Clos	82	8.3 (6.6-10.0)	24	9.4 (5.9-13.0)	45	7.6 (5.5-9.7)	20	10.3 (6.0-14.6)
Staph	55	5.6 (4.1-7.0)	12	4.7 (2.1-7.3)	37	6.2 (4.3-8.2)	9	4.6 (1.7-7.6)
Bacil	16	1.6 (0.8-2.4)	2	0.8 (0-1.9)	11	1.9 (7.7-2.9)	4	2.1 (0.1-4.1)
List	0	0.0	0	0.0	0	0.0	0	0.0
Yer	0	0.0	0	0.0	0	0.0	0	0.0

Positivity per 100 infected individuals with protozoa (total), C. parvum, G. lamblia, or E. histolytica; CI, confidence interval.

The number of cases was counted to each mixed-infected case for individual who has simultaneously several pathogens.

Noro, Norovirus; Rota, Rotavirus (Group A); Adeno, Enteric adenovirus; Astro, Astrovirus; Sal, Sallmonela spp. (Typhimurium and Enteritidis); Shi, Shigella spp.; Vib, Vibrio parahaemolyticus; E. coli, Pathogenic E. coli (STEC, EAEC, EPEC, ETEC, and EIEC); Cam, Campylobacter jejuni; Clo, Clostridium perfringens (alpa toxin, Enterotoxin, or non-toxin); Staph, Staphylococcus aureus (toxin A, B, C, D, and E); Bacil, Bacillus cereus (Hemolysin BL-enterotoxin, Non-hemolytic enterotoxin); List, Listeria monocytogenes; Yer, Yersinia enterocolotica.