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Subgenus classification of Acanthamoeba remains uncertain. Twenty-three reference strains of Acanthamoeba including 18 (neo)type-strains were subjected for classification at the subgenus level by riboprinting. PCR/RFLP analysis of 18S rRNA gene (rDNA). On the dendrogram reconstructed on the basis of riboprint analyses, two type-strains (A. astronyxis and A. tubiashi) of morphological group 1 diverged early from the other strains and were quite distinct from each other. Four type-strains of morphological group 3, A. culbertsoni, A. palestinensis, A. healyi were considered taxonomically valid, but A. pustulosa was regarded as an invalid synonym of A. palestinensis. Strains of morphological group 2 were classified into 6 subgroups. Among them, A. griffini which has an intron in its 18S rDNA was the most divergent from the remaining strains. Acanthamoeba castellanii Castellani, A. quina Vil3, A. lugdunensis L3a, A. polyphaga Jones, A. triangularis SH621, and A. castellanii Ma strains belonged to a subgroup, A. castellanii complex. However, A. quina and A. lugdunensis were regarded as synonyms of A. castellanii. The Chang strain could be regarded as A. hatchetti. Acanthamoeba mauritaniensis, A. divionensis, A. paradivionensis could be considered as synonyms of A. rhysodes. Neff strain was regarded as A. polyphaga rather than as A. castellanii. It is likely that riboprinting can be applied for rapid identification of Acanthamoeba isolated from the clinical specimens and environments.
Figures
Fig. 1 Agarose electrophoretic pattern of PCR products from Acanthamoeba 23 strains. Hind III digested λ phage DNA was used as DNA size marker(M).
Fig. 2 Schematic representation of riboprints of 23 strains of Acanthamoeba by 10 kinds of restriction enzymes. Hae III digested ΦΧ174 DNA was used as the size marker.
Fig. 3 Dendrogram of 23 strains of Acanthamoeba constructed by UPGMA method using Phylip ver. 3.5 based on genetic divergence estimates.
Tables
Table 1 List of 23 strains of Acanthamoeba analysed in this study
Table 2 Proportional homologous (values above the diagonal) and estimates of genetic divergence (values below the diagonal) among 23 strains of Acanthamoeba
References
1.
Bhattacharya D, Helmchen T, Melkonian M. Molecular evolutionary analyses of nuclear-encoded small subunit ribosomal RNA identify an independent rhizopod lineage containing the Euglyphina and the Chlorarachniophyta. J Eukaryot Microbiol 1995;42(1):65–69.
2.
Byers TJ, Hugo ER, Stewart VJ. Genes of Acanthamoeba: DNA, RNA and protein sequences (A Review). J Protozool 1990;37:17–25.
3.
Chung DI, Kong HH, Yu HS, Oh YM, Yee ST, Lim YJ. Biochemical and molecular characterization of a strain KA/S2 of Acanthamoeba castellanii isolated from Korean soil. Korean J Parasitol 1996;34(1):79–85.
5.
Clark CG, Diamond LS. Intraspecific variation and phylogenetic relationships in the genus Entamoeba as revealed by riboprinting. J Eukaryot Microbiol 1997;44(2):142–154.
6.
Clark CG, tin DS, Diamond LS. Phylogenetic relationships among anuran trypanosomes as revealed by riboprinting. J Eukaryot Microbiol 1995;42(1):92–96.
7.
De Jonckheere JF. Isoenzyme and total protein analysis by agarose isoelectric focusing, and taxonomy of the genus Acanthamoeba. J Protozool 1983;30:701–706.
8.
De Jonckheere JF. Ecology of Acanthamoeba. Rev Infect Dis 1991;13:385–387.
9.
Douglas M. Notes on the classification of the amoebe found by Castellani in cultures of a yeast-like fungus. J Trop Med London 1930;33:258–259.
11.
Field BS. The role of amoebae in legionellosis. Clin Microbiol Newsletter 1991;13:92–93.
12.
Gast RJ, Ledee DR, Fuerst PA, Byers TJ. Subgenus systematics of Acanthamoeba: four nuclear 18S rDNA sequence types. J Eukaryot Microbiol 1996;43(6):498–504.
13.
Jadin JB. De la meningoencephalite amibienne et du pouvoir pathogene des amibes limax. Annee Biologique 1973;12:305–342.
14.
Jerome CA, Lynn DH. Identifying and distinguishing sibling species in the Tetrahymena pyriformis complex (Ciliophora, Oligohymenophorea) using PCR/RFLP analysis of nuclear ribosomal DNA. J Eukaryot Microbiol 1996;43(6):492–497.
15.
Jones DB. Acanthamoeba polyphaga keratitis and Acanthamoeba uveitis associated with fatal meningoencephalitis. Trans Ophthalmol Soc UK 1975;95:221–232.
16.
Kim YH, Ock MS, Yun HC, Hwang MY, Yu HS, Kong HH, Chung DI. Close relatedness of Acanthamoeba pustulosa with Acanthamoeba palestinensis based on isoenzyme profiles and rDNA PCR-RFLP patterns. Korean J Parasitol 1996;34(4):259–266.
17.
Kong HH, Chung DI. PCR and RFLP variation of conserved region of small subunit ribosomal DNA among Acanthamoeba isolates assigned to either A. castellanii or A. polyphaga. Korean J Parasitol 996 Jun;34(2):127–134.
18.
Kong HH, Park JH, Chung DI. Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to Acanthamoeba polyphaga. Korean J Parasitol 1995;33(4):331–340.
19.
Ledee DR, Hay J, Byers TJ, Seal DV, Kirkness CM. Acanthamoeba griffini. Molecular characterization of a new corneal pathogen. Invest Ophthalmol Vis Sci 1996;37(4):544–550.
20.
Lewis EJ, Sawyar TK. Acanthamoeba tubiashi n. sp. a new species of fresh-water Amoebida (Acanthamoebidae). Trans Am Microsc Soc 1979;98:543–549.
21.
Ly TM, Muller HE. Ingested Listeria monocytogenes survive and multiply in protozoa. J Med Microbiol 1990;33(1):51–54.
22.
Ma P, Willaert E, Juechter KB, Stevens AR. A case of keratitis due to Acanthamoeba in New York, New York, and features of 10 cases. J Infect Dis 1981;143(5):662–667.
23.
Moura H, Wallace S, Visvesvara GS. Acanthamoeba healyi n. sp. and the isoenzyme and immunoblot profiles of Acanthamoeba spp, groups 1 and 3. J Protozool 1992;39(5):573–583.
25.
Neff RJ. Purification, axenic cultivation, and description of a soil amoeba. Acanth-amoeba sp. J Protozool 1957;4:176–182.
26.
Nei M, Li WH. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci U S A 1979;76(10):5269–5273.
27.
Page FC. Re-definition of the genus Acanthamoeba with descriptions of three species. J Protozool 1967;14(4):709–724.
29.
Pussard M, Pons R. Morphologie de la paroi kystique et taxonomie du genre Acanth-amoeba (Protozoa, Amoebida). Protistologica 1977;13:557–598.
30.
Ray DL, Hayes RE. Hartmannella astrinyxis: a new species of free-living amoeba, Cytology and life cycle. J Morphol 1954;95:159–188.
31.
Reich K. Studien er die Bodenprotozoen palstinos. Arch Protistenkd 1933;79:76–98.
32.
Sawyer TK. Acanthamoeba griffini, a new species ofine amoeba. J Protozool 1971;18:650–654.
33.
Sawyer TK, Nerad TA, Lewis EJ, McLaughlin SM. Acanthamoeba stevensoni n. sp. (Protozoa: Amoebida) from sewage-contami-nated shellfish beds in Rarltan bay, New York. J Protozool 1993;40:742–746.
34.
Samyer TK, Visvesvara GS, Harke BA. Pathogenic amoebas from brackish and ocean sediments, with a description of Acanth-amoeba hatchetti, n. sp. Science 1977;196:1324–1325.
35.
Singh BN. Nuclear division in nine species of small free-living amoebae and its bearing on the classification of the order Amoebida. Philos Trans R Soc Lond B Biol Sci 1952;236:405–461.
36.
Singh BN, Das SR. Studies on pathogenic and nonpathogenic small free-living amoebae and the bearing of nuclear division on the classification of the order amoebida. Philos Trans R Soc Lond B Biol Sci 1970;259:435–476.
37.
Sisson JP, Kemper CA, Loveless M, McShane D, Visvesvara GS, Deresinski SC. Disseminated Acanthamoeba infection in patients with AIDS: Case reports and revisw. Clin Infect Dis 1995;20:1207–1216.
38.
Sogin ML, Gunderson JH, Elwood HJ, Alonso RA, Peattie DA. Phylogenetic meaning of the kingdom concept: an unusual ribosomal RNA from Giardia lamblia. Science 1989;243(4887):75–77.
39.
Stothard DR, Schroeder-Diedrich JM, Awwad MH, Gast RJ, Ledee DR, Rodriguez-Zaragoza S, Dean CL, Fuerst PA, Byers TJ. The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types. J Eukaryot Microbiol 1998;45(1):45–54.
40.
Thom S, Warhurst D, Drasar BS. Association of Vibrio cholerae with fresh water amoebae. J Med Microbiol 1992;36(5):303–306.
41.
Visvesvara GS. Classification of Acanthamoeba. Rev Infect Dis 1991;13:S369–S372.