Abstract
We tried to compare the three kinds of Metagonimus species, M. yokogawai, Metagonimus Miyata type, and M. takahashii, which were known to be distributed in Korea with polymerase chain reaction based-restriction fragment length polymorphism (PCR-RFLP) patterns. We amplified the internal transcribed spacer 1 (ITS1) site of ribosomal RNA and mitochondrial cytochrome c oxidase I (mCOI) gene. The restriction patterns of ITS1 gene with Rsa I, Alu I and Msp I showed multiple fragmented bands of different sizes between three kinds of Metagonimus. In case of mCOI gene, Rsa I and Alu I enzymes produced differentially fragmented band patterns. According to the parsimony analysis of PCR-RFLP patterns, the estimated genetic divergence between M. yokogawai and Metagonimus Miyata type was 0.034880, between Metagonimus Miyata type and M. takahashii was 0.028098, between M. yokogawai and M. takahashii was 0.018179. It is suggested that Metagonimus Miyata type may be separate species and evolutionize at the older time than the other two species.
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- The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes
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BMC Genomics.2022;[Epub] CrossRef - Metagonimus yokogawai Ancient DNA Recovered from 16th- to 17th-Century Korean Mummy Feces of the Joseon Dynasty
Jong Ha Hong, Min Seo, Chang Seok Oh, Jong-Yil Chai, Dong Hoon Shin
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Asian Pacific Journal of Tropical Medicine.2017; 10(2): 165. CrossRef - Fishborne zoonotic heterophyid infections: An update
Jong-Yil Chai, Bong-Kwang Jung
Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef - Molecular phylogeny of trematodes in Family Heterophyidae based on mitochondrial cytochrome c oxidase subunit I (mCOI)
Thapana Chontananarth, Chalobol Wongsawad, Siriwadee Chomdej, Duangduen Krailas, Jong Yil Chai
Asian Pacific Journal of Tropical Medicine.2014; 7(6): 446. CrossRef - Foodborne Intestinal Flukes in Southeast Asia
Jong-Yil Chai, Eun-Hee Shin, Soon-Hyung Lee, Han-Jong Rim
The Korean Journal of Parasitology.2009; 47(Suppl): S69. CrossRef - Molecular phylogeny of parasitic Platyhelminthes based on sequences of partial 28S rDNA D1 and mitochondrial cytochrome c oxidase subunit I
Soo-Ung Lee, Ha-Chung Chun, Sun Huh
The Korean Journal of Parasitology.2007; 45(3): 181. CrossRef - High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand
Pralongyut Sripalwit, Chalobol Wongsawad, Pheravut Wongsawad, Somboon Anuntalabhochai
Experimental Parasitology.2007; 115(1): 98. CrossRef - SUSCEPTIBILITY OF INBRED MOUSE STRAINS TO INFECTION WITH THREE SPECIES OF METAGONIMUS PREVALENT IN THE REPUBLIC OF KOREA
Sang-Mee Guk, Jin-Young Park, Min Seo, Eun-Taek Han, Jae-Lip Kim, Jong-Yil Chai
Journal of Parasitology.2005; 91(1): 12. CrossRef - Sequence comparisons of 28S ribosomal DNA and mitochondrial cytochrome c oxidase subunit I of Metagonimus yokogawai, M. takahashii and M. miyatai
Soo-Ung Lee, Sun Huh, Woon-Mok Sohn, Jong-Yil Chai
The Korean Journal of Parasitology.2004; 42(3): 129. CrossRef - Food-borne intestinal trematode infections in the Republic of Korea
Jong-Yil Chai, Soon-Hyung Lee
Parasitology International.2002; 51(2): 129. CrossRef - Sequencing of simple sequence repeat anchored polymerase chain reaction amplification products of Biomphalaria glabrata
Roberta L Caldeira, Omar S Carvalho, Regina CG Lage, Paula Cristina M Cardoso, Guilherme C Oliveira
Memórias do Instituto Oswaldo Cruz.2002; 97(suppl 1): 23. CrossRef - Surface ultrastructure of Metagonimus takahashii metacercariae and adults
Jong-Yil Chai, Sang-Mee Guk, Eun-Taek Han, Min Seo, Eun-Hee Shin, Woon-Mok Sohn, Sung-Yil Choi, Soon-Hyung Lee
The Korean Journal of Parasitology.2000; 38(1): 9. CrossRef - Molecular Differentiation of Three Species ofMetagonimusby Simple Sequence Repeat Anchored Polymerase Chain Reaction (SSR-PCR) Amplification
H-J. Yang, S-M. Guk, E-T. Han, J-Y. Chai
Journal of Parasitology.2000; 86(5): 1170. CrossRef - A cytogenetic study on human intestinal trematodes of the genus Metagonimus (Digenea: Heterophyidae) in Korea
Soo-Ung Lee, Sun Huh, Gab-Man Park, Jong-Yil Chai
The Korean Journal of Parasitology.1999; 37(4): 237. CrossRef - Surface ultrastructure of Metagonimus miyatai metacercariae and adults
J Y Chai, Y J Kang, S Y Choi, S M Guk, J R Yu, S H Lee
The Korean Journal of Parasitology.1998; 36(4): 217. CrossRef - Expression patterns of proliferating cell nuclear antigen in the small intestine of mice infected with Metagonimus yokogawai and Metagonimus Miyata type
J R Yu, N Myong, J Y Chai
The Korean Journal of Parasitology.1997; 35(4): 239. CrossRef - Different RAPD patterns between Metagonimus yokogawai and Metagonimus Miyata type
J R Yu, J S Chung, J Y Chai
The Korean Journal of Parasitology.1997; 35(4): 295. CrossRef
