Cloning and sequencing of p33 in a Korean isolate of <i>Theileria sergenti</i>

, Kang, S W; , Choi, E J; , Kweon, C H

doi:1997.35.2.105

Korean J Parasito > Volume 35(2):1997 > Article

Original Article


Korean J Parasitol. 1997 Jun;35(2):105-110. English. Published online Jun 20, 1997. http://dx.doi.org/10.3347/kjp.1997.35.2.105
Copyright © 1997 by The Korean Society for Parasitology


Cloning and sequencing of p33 in a Korean isolate of Theileria sergenti
S W Kang,^*E J Choi and C H Kweon
National Veterinary Research Institute, RDA, Anyang 430-016, Korea.

Received February 06, 1997; Accepted March 20, 1997.
Abstract
The gene encoding the 33 kDa piroplasm surface protein of Theileria sergenti isolated in Korea was cloned and the nucleotide sequence was determined by dideoxy chain termination method. The cloned gene corresponds to 869 bp encoding an open reading frame 283 amino acids. Comparison of the sequence between Korean and Japanese isolates showed 99.4% homology rate in the nucleotide sequence and 98.9% homology rate in the amino acid sequence.

Figures


	Fig. 1 Cloning and sequencing procedures of p33 gene of T. sergenti isolated from Korea.


	Fig. 2 Amplification of p33 gene by PCR. M; 1 Kb DNA ladder, lane 1; T. sergenti, lane 2; B. ovata, lane 3; A. marginale.


	Fig. 3 The comparison of nucleotide sequence between KTHP33 (Korean isolate) and JTHP33 (Japanese isolate). Asterisks (*) indicate the sites showing different sequence.


	Fig. 4 The comparison of amino acid sequence between KTHP33 and JTHP33. Arrows indicate the sites showing different amino acid.

Tables


	Table 1 Summary of nucleotide and amino acids comparison of p33 gene between KTHP33 and JTHP33

References


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