A carbohydrate antigen of <i>Clonorchis sinensis</i> recognized by a species-specific monoclonal antibody

, Yong, Tai Soon; , Lee, Jong Seog; , Cho, Sang Nae; , Seo, Jang Hoon; , Park, Hyun

doi:1996.34.4.279

Korean J Parasito > Volume 34(4):1996 > Article

Brief Communication


Korean J Parasitol. 1996 Dec;34(4):279-281. English. Published online Dec 20, 1996. http://dx.doi.org/10.3347/kjp.1996.34.4.279
Copyright © 1996 by The Korean Society for Parasitology


A carbohydrate antigen of Clonorchis sinensis recognized by a species-specific monoclonal antibody
Tai Soon Yong,^*¹Jong Seog Lee,¹Sang Nae Cho,²Jang Hoon Seo,³ and Hyun Park⁴
¹Department of Parasitology, Yonsei University College of Medicine, Seoul 120-752, Korea.
²Department of Microbiology, Yonsei University College of Medicine, Seoul 120-752, Korea.
³Department of Clinical Pathology, Shinheung Junior College, Uijongbu 480-701, Korea.
⁴Biomedical Research Center, Korea Institute of Science and Technology, Seoul 130-650, Korea.

Received September 20, 1996; Accepted November 16, 1996.
Abstract
The enzyme linked immunosorbent assay (ELISA)-inhibition test using a Clonorchis sinensis species-specific mouse monoclonal antibody(MAb), CsHyb 0605-23, showed increased specificity over the conventional ELISA used for serodiagnosis of clonorchiasis. To characterize the corresponding antigen further, the MAb was tested against polysaccharide, protein and glycolipid fractions obtained from a crude extract of C. sinensis adult worms, using chloroform, methanol and phenol extractions. Only the polysaccharide fraction was recognized by the MAb among those fractions. Mild oxidation of the antigen with sodium periodate showed decreased reactivity against the MAb. We concluded that the antigen and antigenic determinants recognized by the MAb are carbohydrates.

Keywords: monoclonal antibody, Clonorchis sinensis, antigenic determinant, carbohydrate, polysaccharide.

Figures

Fig. 1
Seroreactivity of BALB/c mice immunized with crude somatic antigens of C. sinensis adult worms against glycolipid, polysaccharide, or protein fractions of C. sinensis by ELISA. Fractionated antigens were reacted with MAb (CsHyb 0605-23), mouse immune serum, and non-immune mouse serum (negative control), respectively. the MAb reacted with the polysaccharide fraction only, whereas the immune serum showed a high reactivity with both the protein and polysaccharide fractions. The glycolipid fraction of C. sinensis did not show any noticeable reactivity to mouse immune serum or the MAb.


	Fig. 2 The crude antigen of C. sinensis adult worms was coated on the ELISA plate, and treated with sodium periodate at 0, 5, 10, 15, 20 mM. the culture supernatant containing MAb was reacted. Reactivity against MAb decreased, and was does-dependent.

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