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1Department of Parasitology, Yonsei University College of Medicine, Seoul 120-752, Korea. 2Department of Microbiology, Yonsei University College of Medicine, Seoul 120-752, Korea. 3Department of Clinical Pathology, Shinheung Junior College, Uijongbu 480-701, Korea. 4Biomedical Research Center, Korea Institute of Science and Technology, Seoul 130-650, Korea.
The enzyme linked immunosorbent assay (ELISA)-inhibition test using a Clonorchis sinensis species-specific mouse monoclonal antibody(MAb), CsHyb 0605-23, showed increased specificity over the conventional ELISA used for serodiagnosis of clonorchiasis. To characterize the corresponding antigen further, the MAb was tested against polysaccharide, protein and glycolipid fractions obtained from a crude extract of C. sinensis adult worms, using chloroform, methanol and phenol extractions. Only the polysaccharide fraction was recognized by the MAb among those fractions. Mild oxidation of the antigen with sodium periodate showed decreased reactivity against the MAb. We concluded that the antigen and antigenic determinants recognized by the MAb are carbohydrates.
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