Glutamate dehydrogenase antigen detection in <i>Plasmodium falciparum</i> infections

, Dominiguez, Neira de; , Rodriguez-Acosta, Alexis

doi:1996.34.4.239

Korean J Parasito > Volume 34(4):1996 > Article

Original Article


Korean J Parasitol. 1996 Dec;34(4):239-246. English. Published online Dec 20, 1996. http://dx.doi.org/10.3347/kjp.1996.34.4.239
Copyright © 1996 by The Korean Society for Parasitology


Glutamate dehydrogenase antigen detection in Plasmodium falciparum infections
Neira de Dominiguez,^† and Alexis Rodriguez-Acosta^*
Universidad Central de Venezuela Tropical Medicine Institute Immunochemistry Department Apartado 47423 - Caracas 1041 Venezuela.
^†FACULTAD DE FARMACIA-UCV

Received March 28, 1996; Accepted August 17, 1996.
Abstract
The usefulness of malaria diagnosis by Plasmodium falciparum-GDH (NADP⁺), obtained by affinity chromatography, is demonstrated in ELISA assays, testing IgG antibodies against GDH (NADP⁺) from patients with acute malaria, who have had two or more episodes of malaria, or from sera of hyperimmune patients. GDH (NADP⁺) thermal stability was demonstrated in a high heat resistance assay. The immunofluorescence assay demonstrated that anti-culture (P. falciparum) supernatant serum and anti-GDH (NADP⁺) of Proteus spp, recognized epitopes in Venezuelan isolates, and Colombian and Brasilian malarial strains. The antigen is soluble, with high specificity, is a potent immunogen and is thermoresistant.

Figures


	Fig. 1 GDH (NADP⁺) separation procedure from plasma of malaria patients by affinity chromatography.


	Fig. 2 Determination of IgG anti-GDH (NADP⁺) in plasma of malaria patients by direct ELISA.


	Fig. 3 Determination of anti-GDH (NADP⁺) IgG antibody in plasma of malaria patients by indirect ELISA


	Fig. 4 Indirect ELISA evaluation of the GDH (NADP⁺) specificty, compared to other Plasmodium species.


	Fig. 5 Indirect immunofluorescence assay of different preparations.

Tables


	Table 1 Percentages of residual antigenicity of heat treated GDH (NADP⁺)


	Table 2 Mean values (X ± S.D) and sensitivity (%) of GDH (NADP⁺) obtained in two ELISA methods

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