Abstract
For the detection of Cryptosporidium oocysts, fecal samples were collected from 201 calves which showed diarrhea. Among the 201 samples, 29 samples (14.4%) were positive for Cryptosporidium spp. by the DMSO- modified acid-fast stain (MAFS), 23 samples (11.4%) were positive by commercial kit (Meridian Diagnostics, Cincinnati, Ohio) and 23 by the indirect immunofluorescence antibody (IFA) assay employing the monoclonal antibody (mAb C6). When tested by both IFA and MAFS, 20 fecal samples were positive for Cryptosporidium oocysts whereas 169 fecal samples were negative. If the MAFS is considered a standard method for oocyst detection, the IFA showed 69% of sensitivity and 98% of specificity. When tested by both IFA and commercial kit, 22 fecal samples were positive for Cryptosporidium oocysts while 177 samples were negative. One sample tested by IFA was found to be false negative, when compared with the results by commercial kit.
The sensitivity of IFA was calculated as high as 96%; the specificity as 99% and the predictive value was also 99%. In the present study, IFA employing the mAb C6 revealed that 23 samples (11.4%) were positive among the 201 calves showing diarrhea. Of 23 IFA positive samples, 4 samples (5%) showed cryptosporidial oocysts more than 10(5) OPG. Therefore, it is concluded that the calves showing cryptosporidial oocysts more than 10(5) OPG in the feces were highly associated with clinical cryptosporidiosis.
Citations
Citations to this article as recorded by
- W A A V P guideline for evaluating the efficacy of anticoccidials in mammals (pigs, dogs, cattle, sheep)
Anja Joachim, Gertraut Altreuther, Berit Bangoura, Sam Charles, Arwid Daugschies, Barbara Hinney, David S. Lindsay, Hans-Christian Mundt, Marion Ocak, Smaragda Sotiraki
Veterinary Parasitology.2018; 253: 102. CrossRef - Multilocus typing of Cryptosporidium spp. in young calves with diarrhea in Korea
Seung-Hun Lee, Dorene VanBik, Ha-Young Kim, Yu-Ran Lee, Jong Wan Kim, Myeongju Chae, Sang-Ik Oh, Youn-Kyoung Goo, Oh-Deog Kwon, Dongmi Kwak
Veterinary Parasitology.2016; 229: 81. CrossRef - Epidemiological Studies on Intestinal Protozoa in Pigs in Saitama, Japan
Takeshi YUI, Tomoyuki SHIBAHARA, Marina KON, Norishige YAMAMOTO, Mitsuzumi KAMEDA, Hiroyuki TANIYAMA
Japan Agricultural Research Quarterly: JARQ.2014; 48(1): 87. CrossRef - Prevalence of cryptosporidiosis in dairy cattle, cattle-keeping families, their non-cattle-keeping neighbours and HIV-positive individuals in Dagoretti Division, Nairobi, Kenya
Erastus Kange’the, Brigid McDermott, Delia Grace, Cecilia Mbae, Erastus Mulinge, Joseph Monda, Concepta Nyongesa, Julie Ambia, Alice Njehu
Tropical Animal Health and Production.2012; 44(S1): 11. CrossRef - A survey of cryptosporidiosis among 2,541 residents of 25 coastal islands in Jeollanam-do (Province), Republic of Korea
Jae-Hwan Park, Hyo-Jin Kim, Sang-Mee Guk, Eun-Hee Shin, Jae-Lip Kim, Han-Jong Rim, Soon-Hyung Lee, Jong-Yil Chai
The Korean Journal of Parasitology.2006; 44(4): 367. CrossRef - Prevalence of Cryptosporidium among Dairy Cows in Thailand
SATHAPORN JITTAPALAPONG, NONGNUCH PINYOPANUWAT, WISSANUWAT CHIMNOI, CHUTATIP SIRIPANTH, ROGER W. STICH
Annals of the New York Academy of Sciences.2006; 1081(1): 328. CrossRef - Prevalence of cryptosporidiosis among the villagers and domestic animals in several rural areas of Korea
Jae-Ran Yu, Jong-Kyu Lee, Min Seo, Seok-Il Kim, Woon-Mok Sohn, Sun Huh, Hae-Yeon Choi, Tong-Soo Kim
The Korean Journal of Parasitology.2004; 42(1): 1. CrossRef - An epidemiological survey on Cryptosporidium parvum infection of inhabitants in Chorwon-gun, Kangwon-do
Min Seo, Sun Huh, Jong-Yil Chai, Jae-Ran Yu
The Korean Journal of Parasitology.2001; 39(2): 201. CrossRef - Prevalence of Cryptosporidium parvum infection and pattern of oocyst shedding in calves in Japan
S Uga, J Matsuo, E Kono, K Kimura, M Inoue, S.K Rai, K Ono
Veterinary Parasitology.2000; 94(1-2): 27. CrossRef - Detection of Cryptosporidium oocysts in canine fecal samples by immunofluorescence assay
J T Kim, S H Wee, C G Lee
The Korean Journal of Parasitology.1998; 36(2): 147. CrossRef - An epidemiological survey of Cryptosporidium parvum infection in randomly selected inhabitants of Seoul and Chollanam-do
J Y Chai, S H Lee, S M Guk, S H Lee
The Korean Journal of Parasitology.1996; 34(2): 113. CrossRef
