Abstract
A quantitative assay was performed on the effects of gamma-irradiation (30-300 Gy) on intracellular proliferation of Toxoplasma gondii RH tachyzoites in human leukemic HL-60 cells and murine peritoneal macrophages by means of 3H-uracil uptake assay. Infected non- irradiation group (NI) and uninfected group (incubating only host cells) were prepared. The 3H-uracil uptake by tachyzoites of NI group 12-24 hrs after infection was 2,190-4,787 counts per minute for macrophages and 2,967-8,254 for HL-60 cells, whereas the irradiated tachyzoites revealed only 381-703 (100 Gy) and 218-408 (300 Gy) for macrophages, and 1,911-2,618 (30 Gy), 1,253-1,384 (70 Gy), 1,013-1,090 (100 Gy), and 483-588 (300 Gy) for HL-60 cells. The proliferation inhibition rate was similar in macrophages and HL-60 cells, for example, 89-94% and 80-94% respectively by 300 Gy, 12-24 hrs after infection. It is concluded that RH tachyzoites of T. gondii are severely affected by gamma-irradiation in their capability of intracellular proliferation.
Citations
Citations to this article as recorded by
- Exploring the Application of Gamma Radiation-Attenuated Toxoplasma gondii Tachyzoites: A Promising Vaccine Candidate Against Toxoplasmosis
Seyyed Farzad Pourmohammadi, Mohammad Saleh Bahreini, Sara Bajelan, Amin Shamsaei Sarvestani, Bahador Shahriari, Mohammad Mohammadianpanah, Sareh Karbasi, Qasem Asgari
Jundishapur Journal of Microbiology.2025;[Epub] CrossRef - Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis
Eun-Hee Shin, Dong-Hee Kim, Aifen Lin, Jo-Woon-Yi Lee, Hyo-Jin Kim, Myoung-Hee Ahn, Jong-Yil Chai
The Korean Journal of Parasitology.2008; 46(1): 45. CrossRef - Methods for Inactivation of Toxoplasma gondii Cysts in Meat and Tissues of Experimentally Infected Sheep
Fathi A. El-Nawawi, Mohamed A. Tawfik, Raafat M. Shaapan
Foodborne Pathogens and Disease.2008; 5(5): 687. CrossRef - Vaccination with γ‐IrradiatedNeospora caninumTachyzoites Protects Mice Against Acute Challenge withN. caninum
S. RAMAMOORTHY, D. S. LINDSAY, G. G. SCHURIG, S. M. BOYLE, R. B. DUNCAN, R. VEMULAPALLI, N. SRIRANGANATHAN
Journal of Eukaryotic Microbiology.2006; 53(2): 151. CrossRef
