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Serologic response of normal Korean children to Pneumocystis carinii as observed by immunoblot
Hyung Nam Moon,1Sung-Tae Hong,*2 and Soon-Hyung Lee2
1Department of Pediatrics, Ulsan University Asan Medical Center, Seoul 138-040, Korea.
2Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received May 08, 1995; Accepted May 17, 1995.
Abstract
Soluble protein of purified Pneumocystis carinii was prepared from experimentally infected rats. SDS-PAGE of the crude antigen resolved about 20 protein bands from 20 to 200 kDa. Out of them, 116 kDa band strongly reacted and 45-55 and 100 kDa bands reacted weakly to the positive reference human serum from U.S.A. Western blot analysis with sera of 130 normal children and 15 newborns in Korea revealed specific IgG antibody reaction to 40-55 and 116 kDa protein bands. Forty percent (40.0%) of the 145 sera were positive with any of the antigenic protein bands of P. carinii. The positive rate was 56% in 50 males and 33.3% in 48 females. The protein bands 40-55 and 116 kDa from rat P. carinii were confirmed to cross-react with human sera in Korea.
Figures
Fig. 1 Western blot pattern of P. carinii antigen to human sera. Lanes 1 & 6, size marker; 2, P. carinii crude antigen, Coomassie blue stained; 3, serum from a child positive by ELISA; 4, serum from a healthy adult male; 5, positive reference serum from USA.
Fig. 2 Western blot pattern of P. carinii crude antigen to human sera from Korea. 1, Coomassie blue stained bands; 2, size marker; 3, PBS reacted; 4, serum from a newborn infant; 5, serum from an ELISA positive adult; 6 & 7, serum from children; 8, serum from an adult with P. carinii pneumonia.
Tables
Table 1 Anti-P. carinii IgG antibody in serum fo normal Korean children as observed by Western blot
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