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The effect of reinfection with Neodiplostomum seoulensis on the histopathology and activities of brush border membrane bound enzymes in the rat small intestine
Jae-Ran Yu,*1Sung-Tae Hong,2Jong-Yil Chai,2 and Soon-Hyung Lee2
1Department of Parasitology, College of Medicine, Konkuk University, Chungju 380-701, Korea.
2Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received December 28, 1994; Accepted January 22, 1995.
Abstract
Neodiplostomum seoulensis, one of the human intestinal trematodes, was reinfected to albino rats, and worm recovery rates, histopathology and activity changes of the intestinal brush border membrane bound enzymes were observed. The experimental groups were three; uninfected, primary infection and reinfection. The worm recovery rate in the reinfection group was much lower than in the primary infection group 14 days after infection. The duodenal histopathology showed villous atrophy during the first and second week in the primary infection group. In the reinfection group, however, villous changes occurred as early as 3 days after the infection, and the lesion was found healed 7 days after infection. The activities of alkaline phosphatase and sucrase in the duodenum of primary infection rats decreased nearly half of the controls 2 weeks after infection, whereas the activities were unchanged in the reinfection group. However, no changes in the activities were observed in the proximal jejunum between the experimental groups. These findings suggested that a secondary infection of N. seoulensis in rats should make less damage on the intestinal mucosa than a primary infection.
Figures
Fig. 1 Flow chart of the experimental design. Ⓔ: measurement of enzyme activities, Ⓡ: recovery of adult worms, Ⓟ: observation of histopathology
Fig. 2 Recovery rates of N. seoulensis from the experimentally infected rats.
Tables
Table 1 Histopathological findings of the small intestine in rats infected with N. seoulensis
Table 2 Mean enzyme activities in th brush border of small intestine of rats infected with N. seoulensis (unit: mu/mg protein)
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