Oocyst production and immunogenicity of <i>Cryptosporidium baileyi</i> in chickens and mallards

, Rhee, J K; , Jang, B G; , Park, B K

doi:1995.33.1.45

Korean J Parasito > Volume 33(1):1995 > Article

Original Article


Korean J Parasitol. 1995 Mar;33(1):45-54. English. Published online Mar 20, 1995. http://dx.doi.org/10.3347/kjp.1995.33.1.45
Copyright © 1995 by The Korean Society for Parasitology


Oocyst production and immunogenicity of Cryptosporidium baileyi in chickens and mallards
Jae Ku Rhee,^*Beung Gui Jang and Bae Keun Park
Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju 560-756, Korea.

Received December 06, 1994; Accepted January 19, 1995.
Abstract
Two-day-old chickens and mallards were orally inoculated with one of 5 doses varying from 2 × 10² to 2 × 10⁶ of C. baileyi oocysts per individual. Generally, the more oocysts inoculated were, the longer the patent periods were, and the more oocysts shedding were. Meanwhile increasing the inoculative dose, the prepatent periods were shortened except that mallards inoculated with 2 × 10² and 2 × 10³ oocysts failed to produce the oocysts. The more parasites involving oocysts appeared from the chicken in comparison to the mallard. In the chickens challenged with a single dose of 2 × 10⁶ oocysts, a small number of oocysts were detected from feces on days 4-14 after challenge infection (ACI) in all of carrageenan administered groups and in the control groups inoculated with 2 × 10² and 2 × 10³ oocysts. In the mallards, a few oocysts were also recognized on days 5-15 ACI in all of carrageenan treated groups and in the control groups inoculated with 2 × 10², 2 × 10³ and 2 × 10⁴ oocysts. Just prior to challenge infection, phagocytic activity of peritoneal macrophages (Mø) and the number of peripheral Mø in both birds were significantly decreased in the carrageenan treated groups as compared to the control groups. Mild challenge infection in both birds denoted that the immunogenicity of C. baileyi to the birds was very strong, despite Mø blocker carrageenan administration.

Tables


	Table 1 Mean daily oocyst production^a) in chickens inoculated with Cryptosporidium baileyi


	Table 2 Mean daily oocyst production in mallards inoculated with Cryptosporidium baileyi


	Table 3 Effect of carrageenan on the numbers of Mø in peripheral blood and T-cells in splenocytes and phagocytic activity of peritoneal Mø in chickens


	Table 4 Effect of carrageenan on the numbers of Mø in peripheral blood and T-cells in splenocytes and phagocytic activity of peritoneal Mø in mallards


	Table 5 Mean daily oocyst output in chickens after primary and challenge inoculations with Cryptosporidium baileyi (Modified acid-fast staining method)


	Table 6 Mean daily oocyst output in mallards after primary and challenge inoculations with Cryptosporidium baileyi (Modified acid-fast staining method)

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