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Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84 Chronological observation on intestinal histopathology and intraepithelial lymphocytes in the intestine of rats infected with Metagonimus yokogawai
Chronological observation on intestinal histopathology and intraepithelial lymphocytes in the intestine of rats infected with Metagonimus yokogawai
Jong-Yi Chai,*1Tae-Young Yun,1Jin Kim,2Sun Huh,3Min-Ho Choi,1 and Soon-Hyung Lee1
1Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received September 06, 1994; Accepted November 28, 1994.
Abstract
The relationship between the intestinal histopathology and number and position of intraepithelial lymphocytes (IEL) was observed chronologically in the small intestine of rats experimentally infected with Metagonimus yokogawai. Fifteen Sprague-Dawley rats were orally infected each with 3,000 metacecariae, and 3 were kept uninfected for controls. Three rats each were sacrificed on the day 5, 10, 15, 24 and 70 post-infection (PI) and samples of the small intestine, 5 cm, 10 cm, 20 cm and 70 cm posterior to the pylorus were taken. The samples were processed routinely and stained with Giemsa. The intestinal histopathology was severe during the day 5-15 PI and characterized by villous atrophy, crypt hyperplasia, and decrease of villus/crypt height ratio. After the day 24 PI, the intestinal lesions showed some tendency of recovery. The number of IEL increased at the early stage of infection, but decreased thereafter to a lower level than that of controls, with progression of the pathological changes. Then, the IEL number began to increase again after the day 24 PI. In control rats, the great majority of the IEL were located at the basal region of the epithelium. During the early stage of infection, however, a considerable proportion of IEL was found to have moved to the intermediate or apical region of the epithelium. From the above results, it is suggested that the change of IEL number and position during the course of M. yokogawai infection should be closely related to the progression and recovery of the intestinal histopathology.
Figures
Fig. 1 Chronological changes in the number of intraepithelial lymphocytes (IEL) at 4 different portions of the small intestine of rats (S-D) infected with M. yokogawai. In all of the 4 portions the IEL number increased rapidly at 5 days post-infection (PI), but decreased to a lower level than day 0 (control) during 10-24 days PI, and increased again up to slightly higher level than day 0. Values represent the mean IEL count among 500 epithelial cells in 3 rats, S.D. never exceeded 1/3 of the mean value.
Fig. 2 Chronological changes in the number of IEL (average value of 4 portions of the small intestine; Fig. 1) in comparison with the V/C ratio (mean value; Table 1) in rats infected with M. yokogawai. Note that the IEL number and V/C ratio inversely correlate during the early stages (5-15 days) of infection.
Figs. 3-8 Fig. 3. An epithelial layer of the small intestine of a control rat. All intraepithelial lymphocytes (IEL) are located at the basal region (arrows) of the epithelium (× 1,200). Fig. 4. Three IEL (arrows) in a M. yokogawai-infected rat, 5 days post-infection. Each of the IEL is located at apical, intermediate and basal regions of the epithelium (× 1,200). Fig. 5. Jejunum of a 5-day Infection rat. Many IEL (arrows) are located near the intermediate region of the epithelium (× 480). Fig. 6. Two typical IEL (arrows) in the jejunum of a 5-day infection rat. They are located near the perinuclear region of the epithelial cells. The nuclei of IEL are round or oval (× 1,800). Fig. 7. Jejunum of a 5-day infection rat. The villi are severely atrophied, deformed, flat, and fused, and the crypts are hyperplastic. Decreased V/C ratio and increased number of goblet cells are seen (× 120). Fig. 8. Jejunum of a 10-day infection rat, showing two sectioned worms of M. yokogawai. The worms are sucking the villi with their oral suckers. Some parts of the neighboring villi were destroyed, and the villi show increased number of goblet cells (× 120).
Tables
Table 1 V/C ratios in the small intestine of rats infected with M. yokogawai by post-infection days
Table 2 Location of IEL within the epithelium of small intestinal villi in rats infected with M. yokogawai
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