In order to search the fate of Thelohanellus kitauei spores the extrusion rates of the polar filaments were monitored in vitro chronologically. Preserved spores suspended with various solutions at -70 degrees C showed almost the same vigorous pattern as early freezing stages up to 1,750 days after initial preservation. It revealed that the viabilities of some spores suspended with 0.45% and 0.9% NaCl solutions and distilled water at 5 degrees C continued for 1,628 days, 1,614 days and 1,721 days, respectively. And, the life spans of some spores in the previous solutions added with antibiotics at 5 degrees C were 1,628 days, 1,614 days and 1,714 days, respectively.
