Promoting role of <i>Clonorchis sinensis</i> infection on induction of cholangiocarcinoma during two-step carcinogenesis

, Lee, J H; , Yang, H M; , Bak, U B; , Rim, H J

doi:1994.32.1.13

Korean J Parasito > Volume 32(1):1994 > Article

Original Article


Korean J Parasitol. 1994 Mar;32(1):13-18. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1994.32.1.13
Copyright © 1994 by The Korean Society for Parasitology


Promoting role of Clonorchis sinensis infection on induction of cholangiocarcinoma during two-step carcinogenesis
J H Lee,^*H M Yang,U B Bak and H J Rim
Department of Parasitology and Institute for Tropical Endemic Diseases, College of Medicine, Korea University, Seoul 136-705, Korea.

Received January 29, 1994; Accepted March 04, 1994.
Abstract
Chronic Clonorchis sinensis (CS) infection is etiologically related to cholangiocarcinoma (CHCA) in human and animals. This study was carried out to clarify the role of CS infection on dimethylnitrosamine (DMN)-induced cholangiocarcinogenesis. Fifteen hamsters were administered with 15 ppm DMN for 4 weeks and one week later, the hamsters were infected with 15 metacercariae of CS (DMN→CS group). The other 15 hamsters were infected with CS and after 5 weeks they were treated with the drug, praziquantel. Again one week later, the hamsters were administered with DMN (CS→DMN group). The other 15 hamsters were administered with DMN and CS simultaneously (CS+DMN group). Histopathological examination of the livers showed CHCA with papillary or adenomatous hyperplasia of bile ductules in 3 of 15 hamsters of DMN→CS group and in 11 of 15 hamsters of DMN+CS group. These results suggest that CS infection to hamsters may have a promoting effect on the development of CHCA.

Figures


	Fig. 1 Experimental design for study of the role of C. sinensis infection on the development of cholangiocarcinoma.

Tables


	Table 1 Gross findings of the livers in the hamsters in each group


	Table 2 Histopathological changes and tumor incidence in the livers of the hamsters of each group

References


1.	Bhamarapravati N, Thammavit W, Vajrasthira S. Liver changes in hamsters infected with a liver fluke of man, Opisthorchis viverrini. Am J Trop Med Hyg 1978;27(4):787–794.

3.	Chung CS, et al. Korean J Pathol 1976;10(1):33–46.

5.	Farber E. The multistep nature of cancer development. Cancer Res 1984;44(10):4217–4223.

6.	Flavell DJ. Liver-fluke infection as an aetiological factor in bile-duct carcinoma of man. Trans R Soc Trop Med Hyg 1981;75(6):814–824.

7.	Flavell DJ, Lucas SB. Potentiation by the human liver fluke, Opisthorchis viverrini, of the carcinogenic action of N-nitrosodimethylamine upon the biliary epithelium of the hamster. Br J Cancer 1982;46(6):985–989.

8.	Haswell-Elkins MR, Satarug S, Elkins DB. Opisthorchis viverrini infection in northeast Thailand and its relationship to cholangiocarcinoma. J Gastroenterol Hepatol 1992;7(5):538–548.

9.	Hou PC. The pathology of Clonorchis sinensis infestation of the liver. J Pathol Bacteriol 1955;70(1):53–64.

*10.*	Hou PC. The relationship between primary carcinoma of the liver and infestation with Clonorchis sinensis. J Pathol Bacteriol 1956;72(1):239–246.

*11.*	Kim YI, et al. Seoul J Med 1974;15(3):247–255.

*12.*	Kim YI. Liver carcinoma and liver fluke infection. Arzneimittelforschung 1984;34(9B):1121–1126.

*13.*	Lee JH, Rim HJ, Bak UB. Effect of Clonorchis sinensis infection and dimethylnitrosamine administration on the induction of cholangiocarcinoma in Syrian golden hamsters. Korean J Parasitol 1993;31(1):21–30.

*14.*	Lee SH, Shim TS, Lee SM, Chi JG. [Studies On Pathological Changes Of The Liver In Abino Rats Infected With Clonorchis Sinensis]. Korean J Parasitol 1978;16(2):148–155.

*15.*	Lee SY, Lee SH, Chi JG. [Ultrastructural Changes Of The Hepatocytes And Biliary Epithelia Due To Clonorchis Sinensis In Guinea Pigs]. Korean J Parasitol 1978;16(2):88–102.

*16.*	Preston-Martin S, Pike MC, Ross RK, Jones PA, Henderson BE. Increased cell division as a cause of human cancer. Cancer Res 1990;50(23):7415–7421.

*17.*	Thamavit W, Bhamarapravati N, Sahaphong S, Vajrasthira S, Angsubhakorn S. Effects of dimethylnitrosamine on induction of cholangiocarcinoma in Opisthorchis viverrini-infected Syrian golden hamsters. Cancer Res 1978;38(12):4634–4639.

*18.*	Thamavit W, Kongkanuntn R, Tiwawech D, Moore MA. Level of Opisthorchis infestation and carcinogen dose-dependence of cholangiocarcinoma induction in Syrian golden hamsters. Virchows Arch B Cell Pathol Incl Mol Pathol 1987;54(1):52–58.

*19.*	Wykoff DE. Studies on Clonorchis sinensis. III. The host-parasite relations in the rabbit and observations on the relative susceptibility of certain laboratory hosts. J Parasitol 1958;44(5):461–466.