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The nervous system of Fibricola seoulensis by acetylcholinesterase histochemistry
E W Cheon and C H Kim*
Department of Biology, Gyeongsang National University, Chinju 660-701, Korea.
Received August 21, 1993; Accepted September 20, 1993.
Abstract
F. seoulensis were obtained from artificially infected albino rats at 3, 4, 5, 6, 7 days after infection. The worms and metacercariae were washed in physiological saline solution, and fixed with 10% neutral formalin. The acetylcholinesterase (AchE) stained by enzyme histochemistry using acetylthiocholine iodide as substrate. Eserine, iso-OMPA and BW284C51 were used as inhibitors of AchE. The nervous system consists of three pairs longitudinal nerve trunks interconnected with excretory plexus in posterior half, and pharynx and oral sucker in anterior half of metacercariae and adults. The longitudinal nerve trunks are interconnected with transverse commissures and numerous circular commissures. Considerable numbers of circular commissures are interconnected with longitudinal nerve trunks lying on the surface of the worms. At each stage of juvenile worms, AchE and nonspecific cholinesterase activities were observed in the oral sucker, ventral sucker, pharynx and nerve system. Isozymes of AchE in F. seoulensis were separated into the two bands, 69 kDa and 132 kDa. The major band was 69 kDa.
Figures
Figs. 1-6 Fig. 1. Histochemical localization of acetylcholinesterase on metacercariae. Localization of AchE in metacercarae treated with substrate (Acetylthiocholine iodide). ×100. Fig. 2. Metacercariae treated with inhibitor, 10-4 M eserine for 1 hour at room temperature. ×100. Fig. 3. Histochemical localization of acetylcholinesterase of F. seoulensis developed in host for 6 days after infection. Localization of AchE in F. seoulensis treated with substrate (Acetylthiocholine iodide). ×40. Fig. 4.F. seoulensis developed in host for 6 days after infection treated with inhibitor, 10-4 M eserine for 1 hour at room temperature. ×40. Fig. 5. Histochemical localization of acetylcholinesterase on adult (7 days). Localization of AchE in F. seoulensis treated with substrate (Acetylthiocholine iodide). ×40. Fig. 6.F. seoulensis developed in host for 7 days after infection treated with inhibitor, 10-4 M eserine for 2 hour at room temperature. ×40.
Figs. 7-14 Fig. 7. Metacercariae treated with inhibitor, 10-4 M iso-OMPA for 2 hour at room temperature. ×100. Fig. 8. Metacercariae treated with inhibitor, 10-4 M BW284C51 for 2 hour at room temperature. ×100. Fig. 9. Histochemical localization of acetylcholinesterase on F. seoulensis developed in host for 3 days after infection. Localization of AchE in F. seoulensis treated with substrate (Acetylthiocholine iodide). ×40. Fig. 10.F. seoulensis developed in host for 3 days after infection treated with inhibitor, 10-4 M eserine for 2 hour at room temperature. ×40. Fig. 11.F. seoulensis developed in host for 3 days after infection treated with inhibitor, 10-4 M iso-OMPA for 2 hour at room temperature. ×40. Fig. 12.F. seoulensis developed in host for 3 days after infection treated with inhibitor, 10-4 M BW284C51 for 2 hour at room temperature. ×40. Fig. 13. Histochemical localization of acetylcholinesterase on F. seoulensis developed in host for 4 days after infection. Localization of AchE in F. seoulensis treated with substrate (Acetylthiocholine iodide). ×40. Fig. 14.F. seoulensis developed in host for 4 days after infection treated with inhibitor, 10-4 M eserine for 2 hour at room temperature. ×40. Os: oral sucker, P: pharynx, Vs: ventral sucker, Nm: neural mass, Lt: Longitudinal nerve trunk, Mlt: main longitudinal nerve trunk, Cc: circular commissure, Ep: excretory plexus, T: tribocytic organ.
Figs. 15-16 Histochemical localization of acetylcholinesterase on F. seoulensis developed in host for 6 days after infection. Localization of AchE in anterior and posterior portion of developmental adult treated with substrate (Acetylthiocholine iodide). ×200. Os: oral sucker, P: pharynx, Vs: ventral sucker, Nm: neural mass, Lt: Longitudinal nerve trunk, Mlt: main longitudinal nerve trunk, Cc: circular commissure, Ep: excretory plexus, T: tribocytic organ.
Fig. 17 Isozyme patterns of acetylcholinesterase of extracts of F. seoulensis by nondenature PAGE. FE: Treated inhibitor (eserine 10-4 M) in extracts of F. seoulensis, FB: Treated inhibitor (BW284C51 10-4 M) in extracts of F. seoulensis. F: Extracts of F. seoulensis treated with substrate (Acetylthiocholine iodide). MW: marker protein, Co: comassie blue staining, TD: tracking dye
Tables
Table 1 The histochemical localization and relative intensity of acetylcholinesterase activity on developing F. seoulensis in host
References
1.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–254.
2.
Bueding E. Brit J Pharmacol 1952;7:563–566.
3.
Bueding E, Schiller EL, Bourgeois JG. Some physiological, biochemical, and morphologic effects of tris (p-aminophenyl) carbonium salts (TAC) on Schistosoma mansoni. Am J Trop Med Hyg 1967;16(4):500–515.
5.
Bruckner DA, Vage M. The nervous system of larval Schistosoma mansoni as revealed by acetylcholinesterase staining. J Parasitol 1974;60(3):437–446.
6.
Davis BJ. Disc Electrophoresis. II. Method and Application to Human Serum Proteins. Ann N Y Acad Sci 1964;121:404–427.
7.
Edwards AJ, Burt JS, Ogilvie BM. The effect of immunity upon some enzymes of the parasitic nematode, Nippostrongylus brasiliensis. Parasitology 1971;62(2):339–347.
8.
Eranko L. Kinetic observations on cholinesterase activities of rat brain and sympathetic ganglion towards biochemical and histochemical substrates. Acta Physiol Scand 1973;88(1):71–83.
9.
Hammerschlag R, et al. Trends in Neuroscience 1982;5:12–15.
10.
Hart JL. Studies on the nervous system of Tetrathyridia (Cestoda: Mesocestoides). J Parasitol 1967;53(5):1032–1039.
11.
Hong SJ, Lee SH, Seo BS, Hong ST, Chai JY. Studies On Intestinal Trematodes In Korea: IX. Recovery Rate And Development Of Fibricola Seoulensis In Experimental Animals. Korean J Parasitol 1983;21(2):224–233.
12.
Hong ST. Studies On Intestinal Trematodes In Korea: VII. Growth, Development And Recovery Of Fibricola Seoulensis From Experimentally Infected Rats And Mice. Korean J Parasitol 1982;20(2):112–121.
13.
Karnovsky MJ, Roots L. A "Direct-coloring" Thiocholine Method for Cholinesterases. J Histochem Cytochem 1964;12:219–221.
15.
Magee RM, Fairweather I, Johnston CF, Halton DW, Shaw C. Immunocytochemical demonstration of neuropeptides in the nervous system of the liver fluke, Fasciola hepatica (Trematoda, Digenea). Parasitology 1989;98(Pt 2):227–238.
16.
McKay DM, Fairweather I, Johnston CF, Shaw C, Halton DW. Immunocytochemical and radioimmunometrical demonstration of serotonin- and neuropeptide-immunoreactivities in the adult rat tapeworm, Hymenolepis diminuta (Cestoda, Cyclophyllidea). Parasitology 1991;103(Pt 2):275–289.
17.
McLaren DJ. Ultrastructural and cytochemical studies on the sensory organelles and nervous system of Dipetalonema viteae (Nematoda: Filarioidea). Parasitology 1972;65(3):507–524.
18.
Ogilvie BM, Rothwell TL, Bremner KC, Schnitzerling HJ, Nolan J, Keith RK. Acetylcholinesterase secretion by parasitic nematodes. I. Evidence for secretion of the enzyme by a number of species. Int J Parasitol 1973;3(5):589–597.
19.
Probert AJ, Durrani MS. Fasciola hepatica and Fasciola gigantica: total cholinesterase, characteristics, and effects of specific inhibitors. Exp Parasitol 1977;42(1):203–210.
20.
Rotundo RL. Asymmetric acetylcholinesterase is assembled in the Golgi apparatus. Proc Natl Acad Sci U S A 1984;81(2):479–483.
21.
Seo BS, et al. Pathologic findings of small itestinal mucosa of albino rat in Fibricola seoulensis infection. Korean J Parasit 1982;20(2):231–232.
22.
Seo BS, Cho SY, Hong ST, Hong SJ, Lee SH. Studies On Parasitic Helminths Of Korea 5.Survey On Intestinal Trematodes Of House Rats. Korean J Parasitol 1981;19(2):131–136.
23.
Seo BS, Lee SH, Hong ST, Hong SJ, Kim CY, Lee HY. Studies On Intestinal Trematodes In Korea: V. A Human Case Infected By Fibricola Seoulensis (Trematoda: Diplostomatidae). Korean J Parasitol 1982;20(2):93–99.
24.
Seo BS, Rim HJ, Lee CW. Studies on the parasitic helmiths of Korea: I. Trematodes of rodents. Korean J Parasitol 1964;2(1):20–26.
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