Changes of IgE production, splenic helper and suppressor T lymphocytes in mice infected with <i>Paragonimus westermani</i>

, Min, D Y; , Ryu, J S; , Shin, M H

doi:1993.31.3.231

Korean J Parasito > Volume 31(3):1993 > Article

Original Article


Korean J Parasitol. 1993 Sep;31(3):231-238. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1993.31.3.231
Copyright © 1993 by The Korean Society for Parasitology


Changes of IgE production, splenic helper and suppressor T lymphocytes in mice infected with Paragonimus westermani
D Y Min,^*J S Ryu and M H Shin
Department of Parasitology, College of Medicine, Hanyang University, Seoul 133-791, Korea.

Received July 07, 1993; Accepted August 02, 1993.
Abstract
Effects of Paragonims westermani infection were observed in mice on the change of serum IgE level, the number of peripheral eosinophils and the distribution of Thy 1.2⁺ (CD3), L3T4⁺ (CD4), and Lyt-2⁺ (CD8) splenic T lymphocytes without mitogen stimulation. BALB/c mice were infected with 20 metacercariae of P. westermani each. Total serum IgE increased at 3 weeks after the infection and reached a peak on week 4 and maintained high levels of IgE until the 23rd week. Peripheral eosinophil numbers increased at the second week and attained peak level on week 9. The frequency of L3T4⁺ (CD4) and Lyt-2⁺ (CD8) T lymphocytes decreased slightly until 4 weeks after the infection, but not significantly. Absolute number of L3T4+ and Lyt-2⁺ T lymphocytes, and the ratio of L3T4/Lyt-2 were not markedly changed over the period of observation. The frequency of Thy 1.2⁺ (CD3) T lymphocytes in the infected group slightly decreased until 4 weeks after the infection and showed significant reductions at the 2nd and 4th week of the infection (p < 0.05).

Figures


	Fig. 1 Serum IgG antibody levels in mice infected with P. westermani.


	Fig. 2 Total IgE levels in sera of mice infected with P. westermani.


	Fig. 3 Number of peripheral blood eosinophils in mice infected with P. westermani.


	Fig. 4 Spleen weight of mice infected with P. westermani.

Tables


	Table 1 Frequency (%) of positive cells in the spleen of mice infected with Paragonimus westermani by fluorocytometry


	Table 2 Recovery of immature worms in mice infected with P. westermani^a)

References


1.	Ackerman SJ, Gleich GJ, Weller PF, Ottesen EA. Eosinophilia and elevated serum levels of eosinophil major basic protein and Charcot-Leyden crystal protein (lysophospholipase) after treatment of patients with Bancroft's filariasis. J Immunol 1981;127(3):1093–1098.

2.	Ansari A, Williams JF. The eosinophilic response of the rat to infection with Taenia taeniaeformis. J Parasitol 1976;62(5):728–736.

3.	Coffman RL, Carty J. A T cell activity that enhances polyclonal IgE production and its inhibition by interferon-gamma. J Immunol 1986;136(3):949–954.

4.	Coffman RL, Seymour BW, Hudak S, Jackson J, Rennick D. Antibody to interleukin-5 inhibits helminth-induced eosinophilia in mice. Science 1989;245(4915):308–310.

5.	Ikeda T, Fujita K. IGE in Paragonimus ohirai-infected rats: relationship between titer, migration route, and parasite age. J Parasitol 1980;66(2):197–204.

6.	Jarrett EE, Haig DM. Time course studies on rat IgE production in N. Brasiliensis infection. Clin Exp Immunol 1976;24(2):346–351.

7.	Maggi E, Del Prete GF, Tiri A, Macchia D, Parronchi P, Ricci M, Romagnani S. T cell clones providing helper function for IgE synthesis release soluble factor(s) that induce IgE production in human B cells: possible role for interleukin 4 (IL-4). Clin Exp Immunol 1988;73(1):57–62.

8.	Min DY, Ahn MH, Kim KM, Leem MH, Park SY. [The effects of antibodies and complement in macrophage-mediated cytotoxicity on metacercariae of the lung fluke, Paragonimus westermani]. Korean J Parasitol 1990;28(2):91–100.

9.	Min DY, Shin MH, Choi R. [Blastogenesis of splenic lymphocytes to specific antigens and PHA in Paragonimus westermani infected mice]. Korean J Parasitol 1992;30(1):43–48.

*10.*	Min DY, et al. Yonsei J Med Sci 1980;13:94–106.

*11.*	Pfister K, Turner K, Currie A, Hall E, Jarrett EE. IgE production in rat fascioliasis. Parasite Immunol 1983;5(6):587–593.

*12.*	Radermecker M, Bekhti A, Poncelet E, Salmon J. Serum IgE levels in protozoal and helminthic infections. Int Arch Allergy Appl Immunol 1974;47(2):285–295.

*13.*	Rousseaux-Prevost R, Bazin H, Capron A. IgE in experiemental schistosomiasis. I. Serum IgE levels after infection by Schistosoma mansoni in various strains of rats. Immunology 1977;33(4):501–505.

*14.*	Sato Y, Shiroma Y. Peripheral lymphocyte subsets and their responsiveness in human strongyloidiasis. Clin Immunol Immunopathol 1989;53(3):430–438.

*16.*	Sher A, Coffman RL. Regulation of immunity to parasites by T cells and T cell-derived cytokines. Annu Rev Immunol 1992;10:385–409.

*17.*	Sher A, Coffman RL, Hieny S, Scott P, Cheever AW. Interleukin 5 is required for the blood and tissue eosinophilia but not granuloma formation induced by infection with Schistosoma mansoni. Proc Natl Acad Sci U S A 1990;87(1):61–65.

*18.*	Shin MH, Ryu JS, Min DY. [Serum IgE levels in rats infected with Paragonimus westermani]. Korean J Parasitol 1991;29(4):397–401.

*19.*	Sugane K. Eosinophilia in Ascaris suum-reinfected mice. J Helminthol 1988;62(1):51–57.

*20.*	Sugane K, Oshima T. Recovery of large numbers of eosinophils from mice infected with Toxocara canis. Am J Trop Med Hyg 1980;29(5):799–802.

*21.*	Sztein MB, Cuna WR, Kierszenbaum F. Trypanosoma cruzi inhibits the expression of CD3, CD4, CD8, and IL-2R by mitogen-activated helper and cytotoxic human lymphocytes. J Immunol 1990;144(9):3558–3562.

*22.*	Vinayak VK, Khanna R, Kum K, Nain CK. Functional characterization of intestinal intraepithelium & lamina propria lymphocytes from Giardia lamblia infected mice. Indian J Med Res 1989;89:271–281.

*23.*	Yamashita T, Boros DL. Changing patterns of lymphocyte proliferation, IL-2 production and utilization, and IL-2 receptor expression in mice infected with Schistosoma mansoni. J Immunol 1990;145(2):724–731.