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Natural killer cell activity in mice infected with free-living amoeba with reference to their pathogenicity
K H Kim,C O Shin and K Im*
Department of Parasitology and Institute of Tropical Medicine, College of Medicine, Yonsei University, Seoul 120-752, Korea.
Received May 29, 1993; Accepted July 31, 1993.
Abstract
The purpose of this observation was to investigate the natural killer cell activities in mice infected with pathogenic free-living amoeba, Naegleria fowleri and Acanthamoeba culbertsoni according to the infection doses, and infected with non-pathogenic free-living amoeba, Naegleria gruberi. The natural killer cell activity was examined by means of target binding capacity, active NK cell and maximum recycling capacity of the mice after inoculating free-living amoebae with low and high doses. The mice infected with 1,103, 1,105 A. culbertsoni trophozoites showed mortality rates of 6.9% and 65.5%, respectively. The mice infected with 1,104, 1,105 N. fowleri trophozoites showed mortality rates of 5.9% and 72.2%, respectively. The NK cell activities in all experimental groups increased significantly on day 1 after infection as compared with control group, and then remarkably declined thereafter, there was no difference of the cytotoxic activity of the NK cells in mice among inoculation doses of pathogenic free-living amoebae. The target binding capacities of NK cells and percentages of activated NK cells in mice infected with pathogenic free-living amoebae were significantly increased a day after infection, as compared with control group. There was no difference of the maximal recycling capacities of NK cells in all experimental groups as compared with control group. There was significant difference in the cytotoxic activity and single cell cytotoxicity of NK cells between the experimental groups infected with pathogenic free-living amoebae and that infected with non-pathogenic free-living amoebae.
Figures
Fig. 1 An example of figures showing Vmax and Km values. (A) Data from Table 1 are used to examplify the calculation of Vmax. Initial number of target cells (T) is plotted against number of dead target cells (V) at the end of the test. The points fit well to the theoretical Michaelis-Menten saturation curve. (B) A Line Weaver-Burk plot is used to calculate Vmax and km. 1/T = X plotted against 1/V = Y. Data are taken from Table 1 and Fig. 1 (A). Regression analysis is used to obtain a straight line (r = 0.92). Vmax equals the reciprocal of the Y intercept and km equals the negative reciprocal of the X intercept.
Fig. 2 Natural killer cell cytotoxic activity against YAC-1 target cells in mice infected with free-living amoebae.***p<0.05 versus control
Fig. 3 Target binding capacity by single cell cytotoxicity in mice infected with free-lining amoeba.***p < 0.05 versus control
Fig. 4 Fraction of dead conjugates by single cell cytotoxicity in mice infected with free-living amoeba.***p < 0.05 versus control
Fig. 5 Active NK cells by single cell cytotoxicity in mice infected with free-living amoeba.***p < 0.05 versus control
Fig. 6 Values of Vmax of natural killer cell in mice infected with free-living amoeba.***p < 0.05 versus control
Fig. 7 Maximal recycling capacity of natural killer cells in mice infected with free-living amoeba.
Tables
Table 1 An experiment showing data used for calculation of Vmax and Kim*
Table 2 Cumulative number of death in mice inculated intranasally with the free-living amoebae
Table 3 Correlation matrix between NK activity, TBC Active NK cells, Vmax and MRC infected with N. fowleri 1 × 105 trophozoites
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