Natural killer cell activity in mice infected with <i>Acanthamoeba culbertsoni</i>

, Hyun, D K; , Shin, C O; , Im, K

doi:1992.30.2.101

Korean J Parasito > Volume 30(2):1992 > Article

Original Article


Korean J Parasitol. 1992 Jun;30(2):101-112. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1992.30.2.101
Copyright © 1992 by The Korean Society for Parasitology


Natural killer cell activity in mice infected with Acanthamoeba culbertsoni
D K Hyun,C O Shin and K Im
Department of Parasitology, College of Medicine and Institute of Tropical Medicine, Yonsei University, Seoul 120-752, Korea.


Abstract
The natural killer cell activity of splenocytes and TBC, active NK cells, recycling capacity of natural killer cells were observed by means of both the ⁵¹Cr-release cytotoxicity assay and single cell cytotoxicity assay against YAC-1. C3H/HeJ mice were infected intranasally with 1 × 10⁴ or 1 × 10⁵ trophozoites of pathogenic Acanthamoeba culbertsoni. The infected mice showed mortality rate of 34% in 1 × 10⁴ group and 65% in 1 × 10⁵ group, and mean survival time was 16.40 ± 3.50 and 13.20 ± 4.09 days respectively. The cytotoxic activity of natural killer cells of the 2 groups was significantly higher than that of non-infected mice from the 12th hour to the 2nd day after infection, showing the highest on the first day. On the 10th day after infection, the cytotoxic activity of natural killer cells was significantly suppressed as compared with that of the control. There was no significant difference in NK cell cytotoxicity between two infected groups. The target-binding capacity and active NK cells of natural killer cells in 1 × 10⁵ trophozoite infected mice was significantly increased on the 12th hour and the first day after infection as compared with the control group. Maximal recycling capacity (MRC) was not changed during the observation period. The present results indicated that the elevation of natural killer cell activity in the mice infected with A. culbertsoni was due to elevation of target-binding capacity and increased active NK cells of natural killer cells, and not due to the maximal recycling capacity of the individual NK cell, and there was no difference between two experimental dose groups.

Figures

Fig. 1
An example of figures showing Vmax and kim values. (A) Data from Table 1 are used to examplify the calculation of Vmax. initial number of target cells(T) is plotted against number of dead target cells(V) at the end of the test. The points fit well to the theroretical Michaelis-Menten saturation curve. (B) A Line Weaver-Burk plot is used to calculate Vman and Km. 1/T=X plotted against 1/V=Y. Data are taken from Table 1 and Fkg. 1(A). Regression analysis is used to abtain a straight line(r=0.984). Vmax equals the reciprocal of the Y intercept and Km equals the negative reciprocal of the X intercept.


	Fig. 2 Natural killer cell cytotoxic activity against YAC-1 target cells in mice infected with 1×10⁵(□), 1×10⁴(▨) A. culbertsoni trophozoites. ^*p<0.05 versus control


	Fig. 3 Single cell cytotoxic activity against YAC-1 target cells in mice infected with 1×10⁵A. culbertsoni trophozoites. □ TBC ▨ deaed conjugate ^*p<0.05 versus control


	Fig. 4 Active NK against YAC-1 target cells in mice infected with 1×10⁵A. culbertsoni trophozoites. ^*p<0.05 versus control


	Fig. 5 Vmax against YAC-1 target cells in mice infected with 1×10⁵A. culbertsoni trophozoites. ^*p<0.05 versus control


	Fig. 6 Maximal recycling capacity against YAC-1 target cells in enfected with 1×10⁵A. culbertsoni trophozoites.

Tables


	Table 1 An experiment showing data used for calculation of Vmax and Km^*


	Table 2 Cumulative death of mice inoculated intranasally with A. culbertsoni


	Table 3 Natural killer cell cytotoxic activity^** against YAC-1 target cells in mice infected with A. culbertsoni


	Table 4 Single cell sytotoxicity assay^* of natural killer cell, TBC, fraction of dead conjugates and estimation of active NK cells^


	Table 5 Maximum NK potential (Vmax) and maximal recycling capacity^**


	Table 6 Correlation matrix among NK cell cytotoxicty, TBC, active NK cells, Vmax and MRC

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