Abstract
In order to establish a useful cell culture system for T.
gondii, we compared the degree of proliferation of T. gondii tachyzoites among 8 different cell lines; 2 kinds of normal animal cells (MDCK-canine kidney cells; Vero-monkey kidney cells) and 6 kinds of human tumor cells (A 549, PC 14-lung cancer cells; SNU 1, SNU 16, MKN 45-stomach cancer cells; HL-60-promyelocytic leukemia cells), through morphological observation and 3H-uracil uptake assay. The degree of susceptibility to infection with T. gondii tachyzoites was highest in A 549 and PC 14 cells, medium in Vero, HL-60, MDCK and SNU 1, and lowest in SNU 16 and MKN 45 cells. The kinetics of T. gondii multiplication during the post-infection 60 hours were highly dependent upon the dose of tachyzoites administered and the duration of cultivation.
These results show that A 549 and PC 14 are the most suitable cell lines among the 8 tested for the growth and multiplication of T. gondii in vitro.
Citations
Citations to this article as recorded by
- Cytotoxic Effects of Ardisiacrispin A from Labisia pumila on A549 Human Lung Cancer Cells
Yeong-Geun Lee, Tae Hyun Kim, Jeong Eun Kwon, Hyunggun Kim, Se Chan Kang
Life.2024; 14(2): 276. CrossRef - Trans vaccenic acid (trans-11 18:1), a precursor of cis-9, trans-11-conjugated linoleic acid, exerts a direct anti-carcinogenic function in T47D breast carcinoma cells
Jin-Ju Oh, Jae-Sung Lee, Ji-Na Lim, Tao Wang, Sang-Hun Kim, Hong-Gu Lee
Food Science and Biotechnology.2014; 23(2): 641. CrossRef - Toxoplasma gondii: Virulence of tachyzoites in serum free media at different temperatures
M.R. Diab, M.M. El-Bahy
Experimental Parasitology.2008; 118(1): 75. CrossRef - Biology of SNU Cell Lines
Ja-Lok Ku, Jae-Gahb Park
Cancer Research and Treatment.2005; 37(1): 1. CrossRef - USE OF MONOCLONAL ANTIBODIES FOR FLOW CYTOMETRIC DETECTION OF INTRACELLULAR TOXOPLASMA GONDII IN MURINE SPLENIC LYMPHOCYTES
Eun-Hee Shin, Sung-Bum Kim, Ho-Woo Nam, Eun-Taek Han, Jae-Hwan Park, Hye-Jin Ahn, Jong-Yil Chai
Journal of Parasitology.2004; 90(1): 161. CrossRef - Experimental infection of murine splenic lymphocytes and granulocytes with Toxoplasma gondii RH tachyzoites
J Y Chai, J Kook, S M Guk, Y P Chang, C K Yun
The Korean Journal of Parasitology.1997; 35(2): 79. CrossRef
