Antigenic protein fractions of <i>Metagonimus yokogawai</i> reacting with patient sera

, Lee, S C; , Chung, Y B; , Kong, Y; , Kang, S Y; , Cho, S Y

doi:1993.31.1.43

Korean J Parasito > Volume 31(1):1993 > Article

Original Article


Korean J Parasitol. 1993 Mar;31(1):43-48. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1993.31.1.43
Copyright © 1993 by The Korean Society for Parasitology


Antigenic protein fractions of Metagonimus yokogawai reacting with patient sera
Seoung Cheol Lee,Young-Bae Chung,Yoon Kong,Shin-Yong Kang and Seung-Yull Cho^*
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.

Received December 19, 1992; Accepted January 18, 1993.
Abstract
Antibody test is sometimes necessary for the diagnosis of acute human metagonimiasis because eggs may not be detected in stool. The antibody test (ELISA) was evaluated for its significance by reacting human sera from clinically diagnosed metagonimiasis, fascioliasis, clonorchiasis and paragonimiasis with 4 crude extracts of Metagonimus yokogawai (metacercariae), adults of Fasciola hepatica, Clonorchis sinensis and Paragonimus westermani. By ELISA, 10 of 11 metagonimiasis sera showed the highest absorbance (abs.) to the homologous antigen. Cross reactions to M. yokogawai antigen occurred most frequently in clonorchiasis sera. The antigenic protein fractions in M. yokogawai metacercarial extract were observed by SDS-PAGE/immunoblot using patients and control sera together with experimental cat sera. Out of 14 protein bands in the extract, 11 bands were reacting. Cross reacting bands to other trematodiasis sera were frequently observed. Of the reacting bands, 66 and 22 kDa proteins were recognized as specific for metagonimiasis.

Figures


	Fig. 1 SDS-PAGE findings of crude saline extract of M. yokogawai metacercariae. 7.5-15% resolving gel was used for protein separation. M_r: Molecular mass markers, Mc: Crude extract of metacercariae.

Fig. 2
SDS-PAGE/immunoblot findings of crude saline extract of M. yokogawai metacercariae with trematodiases sera. A: Experimental cat, B: Clinical patients of metagonimiasis. C: Clonorchiasis, D: Fascioliasis, E: Paragonimiasis, F: Normal control. Numerical at the left stand for the molecular mass (kDa). Open triangles (◃) show the specific bands for metagonimiasis. Numbers at the top of each lane indicate case number.

Tables


	Table 1 Mean and standard deviation of antibody levels (abs.) as measured by micro-ELISA in patients sera of trematodiases. Protein concentrations of 2.5 µg/ml was standardized in each trematode except for Clonorchis which coated at 5 µg/ml of protein

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