Component proteins and protease activities in excretory-secretory product of sparganum

, Cho, S Y; , Chung, Y B; , Kong, Y

doi:1992.30.3.227

Korean J Parasito > Volume 30(3):1992 > Article

Brief Communication


Korean J Parasitol. 1992 Sep;30(3):227-230. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1992.30.3.227
Copyright © 1992 by The Korean Society for Parasitology


Component proteins and protease activities in excretory-secretory product of sparganum
S Y Cho,Y B Chung and Y Kong
Department of Parasitology, College of Medicine, Chung-Ang University, Seoul 156-756, Korea.


Abstract
Spirometra mansoni plerocercoid (sparganum) was incubated in saline at 4℃ or 37℃ up to 100 hours. Protein contents in the excretory-secretory product (ESP) were rather constant (mean 7.7 mg of protein/gram of sparganum) in the preparations. Reducing SDS-PAGE of ESP showed similar protein subunit compositions with those in crude extract. Antigenic 36 and 31 kDa proteins were major bands in ESP. ESP exhibited specific activities of protease (2.9-5.3 units/mg) at pH 6.0 and pH 7.5. Presence of protease activity in ESP may be a supporting evidence that hitherto known cysteine protease of sparganum is possibly secreted.

Figures

Fig. 1
Reducing SDS-PAGE finding of ESP and crude saline extract of sparganum (80 µg protein in each lane) in linear gradient separating gel of 7.5~15%. Coomassie brilliant blue R-250 stained. (A): ESP collected at 4℃ and crude saline extract of sparganum. Mr: Molecular mass marker, 1: ESP of an hour incubation, 2: ESP of 2-hour incubation, 3: ESP of 5-hour incubation, 4: ESP of an hour incubation, 5: ESP of 12-hour incubation, 6: ESP of 24-hour incubation, 7: ESP of 48-hour incubation, C: crude saline extract. (B): ESP collected at 37℃ and crude extract of sparganum. 1: ESP of 30-minute incubation, 2: ESP of an hour incubation, 3: ESP of 2-hour incubation, 4: ESP of 3-hour incubation, 5: ESP of 24-hour incubation, C: crude extract of sparganum. ★: Antigenic proteins of 36 and 31 kDa of Choi et al. (1988) and Kim et al. (1992), which are recognized not only in crude extract but also in ESP. ◂: Possible cysteine protease of Song and Choi (1992). Estimation of molecular mass of 29 kDa protein in previous reports was changed to 31 kDa when the molecular mass markers of Pharmacia (Sweden) is used instead of Sigma product.

Tables


	Table 1 Protein contents in ESP of sparganum collected in different temperature and time interval


	Table 2 Specific activities of protease in different ESP preparations of sparganum, measured at pH 6.0 and pH 7.5

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