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Comparative study on the effectiveness of modified Kato's cellophane thick smear and Stoll's dilution egg counting technique for quantitative fecal examination of helminth eggs
S J Hong,H C Woo,J H Han and H J Kim*
Department of Parasitology, College of Medicine, Gyeongsang National University, Chinju 660-280, Korea.
Abstract
A total of 197 fecal specimens was prepared for quantitative examination of helminth eggs by modified Kato's cellophane thick smear (M.C.T.S.) and Stoll's dilution egg counting technique (D.E.C.T.). The comparative effectiveness of two techniques was evaluated and conversion function was deduced. The average time required for the microscopic examination on one slide by M.C.T.S. was 12.6 minutes and that of D.E.C.T. was 14.6 minutes. M.C.T.S. showed lower false negative rate than D.E.C.T. in light worm burden cases. Functions to convert the counts obtained by M.C.T.S. to E.P.G. by Stoll's dilution egg counting technique were 47.86 × 100.87 logM.C.T.S. in A. lumbricoides, 41.69 × 100.82 logM.C.T.S. in T. trichiura and 63.10 × 100.85 logM.C.T.S. in C. sinensis. It was suggested M.C.T.S. be better than D.E.C.T. for the quantitative examination of intestinal helminthiases such as A. lumbricoides, T. trichiura, and C. sinensis infections even in the cases with low worm burden.
Tables
Table 1 Log10 values by modified Kato's cellophane thick smear (M.C.T.S.) and by Stoll's dilution egg counting techniques(D.E.C.T.)
References
1.
Beaver PC. Quantitative hookworm diagnosis by direct smear. J Parasitol 1949;35(2):125–135.
2.
Chai JY, Yang YT, Lee SH, Seo BS. The Detectability Of Helminth Eggs From Feces By Cellophane Thick Smear Technique. Korean J Parasitol 1982;20(1):14–20.
3.
Cho SY, Lee SH, Rim HJ, Seo BS. An Evaluation Of Cellophane Thick Smear Technique For Mass Stool Examination. Korean J Parasitol 1969;7(1):48–52.
4.
Kato K, et al. Jpn J Parasitol 1954;3:35.
5.
Katz N, Chaves A, Pellegrino J. A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 1972;14(6):397–400.
6.
Komiya Y, et al. Jpn J Parasitol 1960;9:61–68.
7.
Martin LK, Beaver PC. Evaluation of Kato thick-smear technique for quantitative diagnosis of helminth infections. Am J Trop Med Hyg 1968;17(3):382–391.
8.
Peters PA, El Alamy M, Warren KS, Mahmoud AA. Quick Kato smear for field quantification of Schistosoma mansoni eggs. Am J Trop Med Hyg 1980;29(2):217–219.
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