Changes in blastogenic responses and antibody titers of mice infected with <i>Toxoplasma gondii</i>

, Shin, D W; , Lee, Y H; , Na, Y E; , Kwon, D S

doi:1992.30.2.125

Korean J Parasito > Volume 30(2):1992 > Article

Original Article


Korean J Parasitol. 1992 Jun;30(2):125-131. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1992.30.2.125
Copyright © 1992 by The Korean Society for Parasitology


Changes in blastogenic responses and antibody titers of mice infected with Toxoplasma gondii
D W Shin,Y H Lee,Y E Na and D S Kwon
Department of Parasitology, College of Medicine, Chungnam National University, Daejeon 301-131, Korea.



Abstract
This study was performed to observe the cell-mediated and humoral immune responses in mice which were infected with Beverley, Fukaya and ME49 strain of Toxoplasma gondii, respectively. The blastogenic responses of splenocytes using [³H]-thymidine and serum antibody titers were measured weekly up to 10 weeks after infection. The blastogenic responses of splenocytes treated with concanavalin A and Toxoplasma lysate were significantly declined in the 3 strain groups as compared with the non-infected group (p < 0.05), however lipopolysaccharide-treated blastogenic responses were not significantly different between infected and non-infected groups. The serum IgG antibody titers in the three infected groups increased from 2 weeks after infection, and the serum IgM antibody titers increased until 4 weeks after infection. No significant differences were revealed in blastogenic responses and serum antibody titers among the 3 groups. The present study suggested that cell-mediated immune responses were involved in T. gondii infected mice and blastogenic responses of T lymphocytes were inhibited in acute T. gondii infection.

Figures


	Fig. 1 Stimulation index of concanavalin A-treated splenocytes from Toxoplasma gondii infected and control mice(mean). ^*Stimulation index = (counts per of stimulated splenocytes)/(counts per minute of unstimulated splenocytes)


	Fig. 2 Stimulation index of lipopolysaccharide-treated splenocytes from Toxoplasma gondii infected and control mice(mean). ^*Stimulation index: refer to Fig. 1.


	Fig. 3 Stimulation index of Toxoplasma lysate-treated splenocytes from Toxoplasma gondii infected and control mice(mean). ^*Stimulation index: refer to Fig. 1.


	Fig. 4 Serum IgG antibody titers as measured by ELISA in Toxoplasma gondii infected and control mice(mean). ELISA^*: enzyme-linked immunosorbent assay


	Fig. 5 Serum IgM antibody titers as measured by ELISA in Toxoplasma gondii infected and control mice(mean). ELISA^*: refer to Fig. 4.

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