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In order to observe the antigenic localization in the tissues of Paragonimus westermani of developmental stages, immunogold labeling method was applied using serum of the cats which were infected with isolated metacercariae from Cambaroides similis. The sectioned worm tissues from each developmental stage were embedded in Lowicryl HM 20 medium, stained with infected serum IgG and protein A gold complex (particle size: 12 nm) and observed by electron microscopy.
In the young adult worm tissue of 4 weeks after infection with metacercariae, the gold particles were specifically concentrated on the tegumental syncytium and cytoplasm of the tegumental cells as well as the secretory granules in the parenchymal tissue. The antigenic materials in the adult worm tissue were specifically concentrated on the secretory granules in the parenchymal tissue, the cytoplasm between granules in the vitelline gland and the epithelial lamella in the lumen of the caecum.
Citations
Citations to this article as recorded by
Immunoelectron microscopic localization of partially purified antigens in adult Paragonimus iloktsuenensis Ok-Ran Lee, Pyung-Rim Chung The Korean Journal of Parasitology.2001; 39(2): 119. CrossRef
Localization of worm antigen in Neodiplostomum seoulense by immuno-electronmicroscopy J C Lee, Y Kong, S U Lee, S Huh The Korean Journal of Parasitology.1997; 35(2): 95. CrossRef
Ultrastructural antigenic localization in Paragonimus iloktsuenensis during developmental stage by immunogold labeling method H S Kim, O R Lee The Korean Journal of Parasitology.1995; 33(4): 365. CrossRef
Protein composition and antigenicity of the tegument from Paragonimus westermani S I Kim, S Y Cho The Korean Journal of Parasitology.1993; 31(3): 269. CrossRef
Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies T S Yong, J H Seo, I S Yeo The Korean Journal of Parasitology.1993; 31(2): 141. CrossRef
Antigenic localities in the tissues of Metagonimus yokogawai in the period of growth H J Rim, S J Kim, M G Yang The Korean Journal of Parasitology.1992; 30(4): 309. CrossRef
