Observation of mucosal pathology after praziquantel treatment in experimental <i>Fibricola seoulensis</i> infection in rats

, Lee, S H; , Kim, B I; , Hong, S T; , Sohn, W M; , Chai, J Y

doi:1989.27.1.35

Korean J Parasito > Volume 27(1):1989 > Article

Original Article


Korean J Parasitol. 1989 Mar;27(1):35-40. Korean. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1989.27.1.35
Copyright © 1989 by The Korean Society for Parasitology


Observation of mucosal pathology after praziquantel treatment in experimental Fibricola seoulensis infection in rats
S H Lee,B I Kim,S T Hong,W M Sohn,^* and J Y Chai
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Seoul 110-460, Korea.
Department of Parasitology^*, College of Medicine, Inje University, Pusan 614-112, Korea.


Abstract
It is well known that the duodenum of mice or rats infected with Fibricola seoulensis shows atrophy of villi (shortening, blunting, widening, fusion) and hyperplasia of crypts. This study was performed to observe healing process of these pathological changes after deworming with anthelmintic treatment. Albino rats infected each with 1,000 metacercariae of F. seoulensis were treated with single dose of 10 mg/kg praziquantel on day 15 post-infection. On day 1, 3, 5, 7, 15, 21 and 28 after the treatment, they were sacrificed and their duodenums were histopathologically studied. Control (uninfected) rats showed their normal finger-like projections of duodenal villi and well arranged crypts. In comparison, untreated (infected) controls revealed severe mucosal changes characteristic of villous atrophy and crypt hyperplasia in their duodenum. The damaged duodenal mucosa was found to restore its normal morphology after praziquantel treatment; until day 3 post-treatment the mucosa was severely atrophied; on day 5 long and slender villi sometimes appeared among the fused and stout ones; after day 15 the villi were in their normalizing process. From this experiment, it was shown that the mucosal changes in the duodenum of rats caused by F. seoulensis infection were completely reversible in 21-28 days after anthelmintic treatment.

Figures


	Fig. 1 Section of the duodenum of a control (uninfected) rat, showing well arranged villi and crypts with their normal shape. ×48.


	Fig. 2 Section of the duodenum of an infected control rat, showing remarkably destroyed mucosa with severely deformed villi and hyperplastic crypts due to F. seoulensis infection. ×40.


	Duodenal section of 1 day post-treatment group. ×48.


	Fig. 4 Duodenal section of 5 day post-treatment group. ×48.


	Fig. 5 Duodenal section of 15 day post-treatment group, showing villi and crypts restoring their normal configurations. ×48.


	Fig. 6 Duodenal section of 28 day post-treatment group, revealing nearly normalized villi and crypts with high V/C ratio. ×56.

Tables


	Table 1 Number of rats used in this study


	Table 2 Histopathological findings of the duodenum of albino rats infected with F. seoulensis and changing patterns after treatment with praziquantel

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