This study was carried out in order to find out the valuable measure to kill the parasite eggs in night soil. The fresh eggs of Ascaris put into human stool were irradiated with Cobalt 60 of 200,000 to 1,000,000 rad and cultured in test tubes at 25-30 C, washing with 2 percent formalin solution every 24 hours. The continuous development of those eggs were observed under microscope and the proprotions of developed eggs were compared with those of control groups.
The major result can be summarized as follows: In general, The eggs in stool developed poorly than the eggs in saline.
If the eggs were irradiated with the larger dose of Cobalt 60, the proportion of developed eggs were reduced subsequently. The eggs irradiated with the dose of 1,000,000 rad in saline developed in the proportion of 15.5 percent, whereas irradiated with 200,000 rad 94 percent developed in 4 weeks. The 44.5 percent of eggs in stool irradiated with 200,000 rad developed after 4 weeks, 30.5 percent with 300,000 rad, 25 percent with 500,000 rad and 3.5 percent with 1,000,000 rad respectively. The effective minimum dose of Cobalt 60 irradiation to kill the Ascaris eggs in stool was estimated 1,000,000 rad. Further examination will be required to observe the infectivity of irradiated Ascaris eggs to animals and to evaluate the effect from the standpoint of sanitary engineerings.
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The experiments on the infectivity to mice of the Ascaris eggs irradiated with Cobalt60 Ok Ran Lee, Baek Hyun Yun, Won Young Choi The Korean Journal of Parasitology.1970; 8(3): 90. CrossRef
In the summer of 1969 from July through October, authors carried out a study of susceptibility in adult female bed bug(Cimex lectularius) in order to ascertain any development of resistance to insecticides in Korea. The test to DDT and Dieldrin was performed in laboratory by the continuous exposure of residues method, with standard impregnated paper used for adult mosqitoes. Some results so far obtained can be summarised as follows: LC(50) were 2.839% in DDT and 0.167% in Dieldrin. The resistance has been arisen 2.4-2.9 times in DDT and 1.6-2.4 times in Dieldrin as compared with London laboratory strain, but they were susceptible 1.4 times in DDT and 180 times in Dieldrin as compared to Israel resistance strain.
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The duration of infection, frequency of eating raw fresh water fishes, and a series of liver function tests were studied in a group of 23 Korean symptomless cases with Clonorchis sinensis, and compared with those in a control group of uninfected persons. Of 23 lightly infected cases, 11 cases who had been infected for 10 to 20 years was found the highest in percentage(47.8), and 2 had been infected for over 30 years. The frequency of eating raw fresh water fishes ranged from 2 to 35 times. The range of Stoll egg-counts in 23 cases varied from 600 to 8,600 eggs per gram of feces and the average value was approximately 2,800 eggs. A series of liver function tests examined showed normal value in all cases. These results showed no difference in either infected group and its contol group.
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The Trichomonas immobilization-agglomeration reaction was studied using the sera from women with vaginal trichomoniasis and from rabbits inoculated with cultures of T. vaginalis. It was found that the greatest amount of immobilization occurred at 25 to 30 minutes, and that inactivation of the sera did not affect the results. An evidence of antigenic differences between T. vaginalis, T.
hominis and C. albicans was confirmed. The immobilization reaction was positive in 80.3% of the 71 T. vaginalis positive cases, but in only 7.7% of the 77 persons of T.
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Detecting eggs from feces and/or sputum is probably closely associated with many factors such as degree or intensity of infection, physiological status of the host(age, eating habit and duration of residence in the area), the duration of infection for the parasite (age and reproductive activity of flukes), and methods of collecting specimens and technique of examination. Neverthless, it is difficult to determine which factor plays the most inportant role in detecting eggs except comparison of factual result obtained by standardized techniques. The purpose of the study was to find out which method would give better result for detection of eggs, and to estimate what proportion of patients would be missed when the method selected is used. On a single examination of both specimens, stool and sputum, collected from the same person, sputum examination was found to be superior to stool examination for detection of eggs; 37 of 40 egg positives had eggs in sputum whereas only 21 of 40 in stool. Repeated sputum examination on the same subject in spaced time gave higher overall egg detection rate; in the first examination for all skin reaction positives, the detection rate was 36.8%, in the second examination on those who had negative results in the first examination, it was 11.6% among 602 persons examined, and 5.3 percent of 95 persons who were negative in previous two examinations.
Thus, repeated sputum examinations (three times) increased the overall detection rate to 48.5% from 36.8%. According to the result obtained through this study, it would be worthwhile to recommend repeated sputum examinations at least three times on the same subject even if collecting second and third sputum is quite difficult problem in mass survey; about 12% of total patients who can be detected as positive by three times repeated examinations shall be missed if only a single sputum examination is done.
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Paragonimus and paragonimiasis in Korea D W Choi The Korean Journal of Parasitology.1990; 28(Suppl): 79. CrossRef
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Prevalence of intestinal parasites in Korea Chong Hwan Kim, Chung Hee Park, Hwa Jun Kim, Hyung Bock Chun, Hong Kee Min, Tae Young Koh, Chin Thack Soh The Korean Journal of Parasitology.1971; 9(1): 25. CrossRef
A parasitological study was performed with 452 malaria patients evacuated from South Vietnam by examinations of their peripheral blood. Results were as follows: The peripheral blood examinations revealed that 52.0% of the examined have parasitemia, of which 95.3% was P. falciparum, one case of P. vivax and the other 10 patients were mixed infected. Neither P. malaria nor P. ovale were found. A total of 1,500 thick and thin blood films was prepared and 707 slides of them (47.1%) showed positive. In P.
falciparum, ring forms were found most frequently and the next was gametocytes. Eighty slides (50%) showed mixed together with both ring form and gametocytes. All of the erythrocytic stages were seen in three slides of P. vivax.
Weekly periodical examinations showed 233.8 parasite density every 1,000 W.B.C count in average, while occasional at fever attacks 531.7.Size of gametocytes in P. falciparum was 9.31(+/-0.89) by 2.16(+/-0.53) in macrogametocyte and 6.61(+/-0.82) by 2.51(+/-0.35) in microgametocyte. Their sex ratio was 100 : 92. Repeated blood examinations showed increased detection rates. The positive rate of parasitemia was 52.0% in single examination, increasing in succession with repetitions.
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Imported Malaria in Korea: a 13-Year Experience in a Single Center Hae Suk Cheong, Ki-Tae Kwon, Ji-Young Rhee, Seong Yeol Ryu, Dong Sik Jung, Sang Taek Heo, Sang Yop Shin, Doo Ryun Chung, Kyong Ran Peck, Jae-Hoon Song The Korean Journal of Parasitology.2009; 47(3): 299. CrossRef
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A survey of intestinal parasites was performed in South Vietnam. Samples were collected from 717 Vietnameses, 1,933 U.S. Forces, 433 stool specimens of Korean Troops in South Vietnam. And 114 of Korean Army patients in home land were also examined as a control. Findings were discussed with others and summarized as follows: A parasitic rate, incidence in general, was 44.8 %. Koreans in South Vietnam showed 82.4 % of infection rate, Vietnamese 64.6% and U.S.
Forces 26.1 %. Korean home patients revealed 93.9 %.
Following 10 species of helminths ova and 5 kinds of protozoa were found: Ascaris lumbricoides, Hookworm, Trichocephalus trichiurus, Strongyloides stercoralis, Trichostrongylus orientalis, Enterobius vermicularis, Metagonimus yokogawai, Clonorchis sinensis, Hymenolepis nana, Taenia sp., Entamoeba histolytica, Entamoeba coli, Endolimax nana, Giardia lamblia and Trichomonas hominis. The incidence of Ascaris lumbricoides among Koreans in South Vietnam was 15.9 %, much less than Korean home patients. The infection rate of A. lumbricoides in Saigon residents was 47.2% but in Chulai 36.8% and in Quinhon 35.8 %.
Trichocephalus trichiurus was most frequently found in Koreans. Quinhon residents showed 78.7 % positive in hookworn infection and Chulai 24.7 %. In Saigon, on the contrary, infection rate was only 3.1 %. No Strongyloides stercoralis, but two cases of Trichomonas hominis were found among Koreans in South Vietnam. U.S. Forces showed higher incidences than Koreans and Vietnameses in the incidences of Entamoeba histolytica and Endolimax nana. Most of the U.S.
Forces revealed single infection. Korean home patients showed the highest per centage of polyparasitism.
Non-autochthonous infections in Korea could not be found among Koreans in this survey.
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Rhabditis elongata Schneider, 1866 from students in Korea Won Koo Lee, Won Yong Choi, Ok Ran Lee The Korean Journal of Parasitology.1978; 16(2): 113. CrossRef
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The present studies were carried out, under natural room condition from June to July in 1969. The period required for growth of eggs and larva varied according to various temperatures and methods of rearing. The average number of eggs laid by the Culex pipiens pallens was 137 per egg raft.
The period of gonotrophic cycle was from 3 to 6 days: incubation period of eggs was from 1.5 to 2 days: larva period was from 10 to 14 days: pupa period was from 2 to 3 days. Accordingly, the period from the beginning of gonotrophic cycle to the adult mosquitos in next generation required 25 days at the longest and 16.5 days at the shortest.
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