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Volume 38(3); September 2000

Mini Review

Unstable vivax malaria in Korea
Han-Il Ree
Korean J Parasitol 2000;38(3):119-138.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.119

Korean vivax malaria had been prevalent for longtime throughout the country with low endemicity. As a result of the Korean war (1950-1953), malaria became epidemic. In 1959-1969 when the National Malaria Eradication Service (NMES) was implemented, malaria rates declined, with low endemicity in the south-west and south plain areas and high endemic foci in north Kyongsangbuk-do (province) and north and east Kyonggi-do. NMES activities greatly contributed in accelerating the control and later eradication of malaria. The Republic of Korea (South Korea) was designated malaria free in 1979. However, malaria re-emerged in 1993 and an outbreak occurred in north Kyonggi-do and north-west Kangwon-do (in and/or near the Demilitarized Zone, DMZ), bordering North Korea. It has been postulated that most of the malaria cases resulted from bites of sporozoite-infected females of An. sinensis dispersed from North Korea across the DMZ. Judging from epidemiological and socio-ecological factors, vivax malaria would not be possible to be endemic in South Korea. Historical data show that vivax malaria in Korea is a typical unstable malaria. Epidemics may occur when environmental, socio-economical, and/or political factors change in favor to malaria transmission, and when such factors change to normal conditions malaria rates become low and may disappear. Passive case detection is a most feasible and recommendable control measure against the unstable vivax malaria in Korea in cost-effect point of view.

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Original Articles

Mucosal mast cell responses in the small intestine of rats infected with Echinostoma hortense
Insik Kim, Jae-Aee Im, Kyu-Je Lee, Yong-Suk Ryang
Korean J Parasitol 2000;38(3):139-143.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.139

Mucosal mast cell (MMC) responses and worm recovery rates in rats infected with Echinostoma hortense were investigated from day 3 to day 56 post-infection (p.i.). Experimental infected group showed apparently higher number of MMC in each part of the small intestine than that of the control group. The number of MMC in the duodenum increased gradually after the infection and reached a peak on day 35 p.i. Thereafter, the number of MMC continued to decrease at a slow pace. The kinetics of MMC responses in the upper and lower jejunum were similar to that of the duodenum, but the number of MMC in the jejunum was lower. The worm recovery rate decreased with respect to time of which it was markedly reduced on day 49 and 56 p.i. The duration in which a high number of MMC appeared was similar to that in which a low rate in worm recovery was recorded. These results indicate that intestinal mastocytosis may play an important role in the expulsion of E. hortense.

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IgG antibody responses in early experimental sparganosis and IgG subclass responses in human sparganosis
Young Bae Chung, Yoon Kong, Hyun Jong Yang, Seung-Yull Cho
Korean J Parasitol 2000;38(3):145-150.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.145

Antigenic components in the crude extracts of Spirometra mansoni plerocercoid were analyzed in early experimental infections and in IgG subclass observed in clinical sparganosis. By IgG immunoblot, sera obtained serially from experimental mice, fed 5 spargana each, were reacted with the crude extracts. Protein bands at 36-26 kDa and 103 kDa showed positive reactions since two weeks after infection. In a differential immunoblot, in which a monospecific antibody against sparganum chymase at 36 kDa was pre-treated, the reactions at 36-26 kDa disappeared, indicating that the sparganum chymase and its degradation products invoked IgG antibody reactions. When 69 patients sera of human sparganosis were examined for their IgG subclass responses, IgG4 levels showed the highest reaction which was followed by IgG1. The IgG4 antibody also reacted mainly with 36-31 kDa protease. These results indicate that 36 kDa chymase of S. mansoni plerocercoid is the main antigenic component inducing IgG antibody response in early stage of experimental sparganosis and for specific IgG subclass reactions in human sparganosis.

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    Soji Fukumoto, Miki Hiroi, Paramasari Dirgahayu, Kazutoyo Miura, Sayuri Tademoto, Hitoshi Otsuki, Yoshihiro Ohmori
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    PLoS Neglected Tropical Diseases.2012; 6(11): e1899.     CrossRef
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    Moizur Rahman, Eung-Goo Lee, Young-An Bae
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  • Production of Polyclonal Antibodies against the Tegument of Sparganum (Plerocercoid of Spirometra mansoni) and Its Immunolocalization
    Hyun-Jong Yang
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    Malinee T. Anantaphruti, S. Nuamtanong, P. Dekumyoy
    Tropical Medicine & International Health.2005; 10(10): 1013.     CrossRef
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Genetic heterogeneity of Pneumocystis carinii from rats of several regions and strains
Byung-Suk Chung, Yun-Kyu Pars, Sun Huh, Jae-Ran Yu, Jin Kim, Xiaohua Shi, Sang Rock Cho, Soon-Hyung Lee, Sung-Tae Hong
Korean J Parasitol 2000;38(3):151-158.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.151

Pneumocystis carinii is a major opportunistic pathogen which has been found in the lungs of a wide variety of mammalian host species, and the fact suggests the possibility of intraspecific variation. Until now, P. carinii from different mammalian species are differentiated as subspecies, and the rats are known to be infected by two subspecies. The present study investigated genetic heterogeneity of P. carinii isolates from two strains of rats in Korea and China by molecular karyotyping, RFLP and sequencing analysis. Karyotypes of P. carinii were grouped into three, two from two strains of rats in Korea and one from rats in China. However RFLP of PCR product of ribosomal and MSG gene of the P. carinii isolates showed same pattern. The sequence homology rates of α-tubulin DNA of the P. carinii isolates were 96% in Seoul Wistar rats, 93% in Seoul Sprague-Dawley rats, and 85% in Chinese Sprague-Dawley rats. The present finding confirmed that P. carinii from rats in Korea are grouped into two karyotype strains which are different from that of P. carinii from rats in China. The Chinese isolate shows a little different sequences of α-tubulin DNA.

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  • Intraspecific variability in several isolates of Philasterides dicentrarchi (syn. Miamiensis avidus), a scuticociliate parasite of farmed turbot
    Belén Budiño, Jesús Lamas, María P. Pata, Juan A. Arranz, Manuel L. Sanmartín, José Leiro
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    Jae-Seok Kim, Yong-Kyun Kim, Ji Young Park, Eun Kyung Mo, Han Sung Kim, Wonkeun Song, Hyoun Chan Cho, Kyu Man Lee
    Chonnam Medical Journal.2008; 44(2): 82.     CrossRef
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Purification of a 68-kDa cysteine proteinase from crude extract of Pneumocystis carinii
Min-Ho Choi, Byung-Suk Chung, Young-Bae Chung, Jae-Ran Yu, Sang Rock Cho, Sung-Tae Hong
Korean J Parasitol 2000;38(3):159-166.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.159

The present study intended to verify activities of cysteine proteinase of Pneumocystis carinii from rats and to purify the enzyme. In order to exclude the contamination of host-derived enzymes, concentrates of P. carinii was primarily treated with a mixture of proteinase inhibitors before lysis of P. carinii. A 68-kDa cysteine proteinase was finally purified from the crude extract of P. carinii by 4 sequential chromatographic methods. The enzyme showed an optimal activity at pH 5.5 in 0.1 M sodium acetate, and its activity was specifically inhibited by L-trans-epoxysuccinylleucylamido (4-guanidino) butane (E-64) and iodoacetic acid, suggesting that the enzyme is a cysteine proteinase. The 68-kDa proteinase weakly digested macromolecules such as collagen, hemoglobin and fibronectin. The present study demonstrated the activity of cysteine proteinase at the 68-kDa band of P. carinii, and purified and characterized the molecule.

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  • Comparative Genomics Suggests that the Fungal Pathogen Pneumocystis Is an Obligate Parasite Scavenging Amino Acids from Its Host's Lungs
    Philippe M. Hauser, Frédéric X. Burdet, Ousmane H. Cissé, Laurent Keller, Patrick Taffé, Dominique Sanglard, Marco Pagni, Jason E. Stajich
    PLoS ONE.2010; 5(12): e15152.     CrossRef
  • Characterization of a Novel ADAM Protease Expressed byPneumocystis carinii
    Cassie C. Kennedy, Theodore J. Kottom, Andrew H. Limper
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Case Report

A case of chronic cerebral paragonimiasis westermani
Shin-Yong Kang, Tae-Kwon Kim, Tae Yun Kim, Young-Il Ha, Sun-Wook Choi, Sung-Jong Hong
Korean J Parasitol 2000;38(3):167-171.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.167

We report a chronic cerebral paragonimiasis from a 41-year-old Korean man who complains a headache and weakness of left motor neuron components. Magnetic resonance images of the brain revealed conglomerates of multiple ring-like enhancements in temporo-occipital and frontal lobes of the right hemisphere. An intradermal test for paragonimiasis westermani was positive. The patient was born near an endemic area of paragonimiasis and used to eat boiled or grilled freshwater crayfish in his childhood. Nodules in the brain were resected through craniotomies. The eggs of P. westermani were identified pathologically and parasitologically in the calcified necrotic lesions. Examinations on sputum and fecal specimens for the eggs of P. westermani were shown to be negative and a chest radiograph was normal. It is presumed that the brain lesions were formed by P. westermani approximately 30 years ago.

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    Expert Review of Neurotherapeutics.2023; 23(3): 237.     CrossRef
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    Seok Woo Moon, Taeho Kim
    The Korean Journal of Parasitology.2022; 60(5): 353.     CrossRef
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    Tae Im Kim, Se-Ra Oh, Fuhong Dai, Hyun-Jong Yang, Sang-Do Ha, Sung-Jong Hong
    Parasitology Research.2017; 116(3): 1003.     CrossRef
  • Cerebral Paragonimiasis Presenting with Sudden Death
    Gary W. Procop, Annie Cowell, Sharon L. Reed, Deirdre E. Amaro, Marion J. Tuohy, Jacquelyn Morhaime
    The American Journal of Tropical Medicine and Hygiene.2016; 95(6): 1424.     CrossRef
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    Macpherson Mallewa, Jo M. Wilmshurst
    Seminars in Pediatric Neurology.2014; 21(1): 19.     CrossRef
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    Jia‐Yan Wu, Bao‐Rong Zhang, Guo‐Hua Zhao
    CNS Neuroscience & Therapeutics.2013; 19(9): 734.     CrossRef
  • The Return of an Old Worm: Cerebral Paragonimiasis Presenting with Intracerebral Hemorrhage
    Eun Jung Koh, Seung-Ki Kim, Kyu-Chang Wang, Jong-Yil Chai, Sangjoon Chong, Sung-Hye Park, Jung-Eun Cheon, Ji Hoon Phi
    Journal of Korean Medical Science.2012; 27(11): 1428.     CrossRef
  • Excretory–secretory products from Paragonimus westermani increase nitric oxide production in microglia in PKC-dependent and -independent manners
    Youngnam Jin, In Young Choi, Chunsook Kim, Suyoung Hong, Won-Ki Kim
    Neuroscience Research.2009; 65(2): 141.     CrossRef
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    Carlos Graeff-Teixeira, Ana Cristina Arámburu da Silva, Kentaro Yoshimura
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    André Paugam
    Revue Francophone des Laboratoires.2008; 2008(399): 41.     CrossRef
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    Carlos S. Restrepo, Abhijit A. Raut, Roy Riascos, Santiago Martinez, Jorge Carrillo, Srinivasa R. Prasad
    Seminars in Roentgenology.2007; 42(1): 37.     CrossRef
  • Excretory-Secretory Products Produced by Paragonimus westermani Differentially Regulate the Nitric Oxide Production and Viability of Microglial Cells
    Youngnam Jin, Jae-Chul Lee, In Young Choi, Eun A. Kim, Myeong Heon Shin, Won-Ki Kim
    International Archives of Allergy and Immunology.2006; 139(1): 16.     CrossRef
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    Antonio José da Rocha, Antonio Carlos Martins Maia, Nelson Paes Diniz Fortes Ferreira, Lázaro Luís Faria do Amaral
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  • Paragonimiasis: a view from Columbia
    Iván D Vélez, Jorge E Ortega, Luz E Velásquez
    Clinics in Chest Medicine.2002; 23(2): 421.     CrossRef
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    Fukumi Nakamura-Uchiyama, Hiroshi Mukae, Yukifumi Nawa
    Clinics in Chest Medicine.2002; 23(2): 409.     CrossRef
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Brief Communications

Postmetacercarial changes in Echinostoma caproni maintained in a defined medium plus calf serum
Bernard Fried, Aditya Reddy
Korean J Parasitol 2000;38(3):173-175.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.173

The present study examined postmetacercarial changes in the excysted metacercariae of Echinostoma caproni maintained in the defined medium Mixture 199 plus 20% calf serum for 7 days at 41℃. The gas phase was atmospheric air. Each culture was inoculated with 25 excysted metacerariae. Cultures were maintained upright in closed 15 ml plastic centrifuge tubes each containing 10 ml of medium plus 200 units of penicillin/ml and 200 ?g of streptomycin/ml. By 4 days in culture, most metacercariae had voided their excretory concretions. Organisms were clumped or solitary at the bottom of the cultures. Many organisms showed flaring of the oral collar and extension of both the collar and tegumentary spines. By 4 days in culture, posterior protuberances or bumps were noted on many of the organisms and some organisms showed abnormal vesicular growths or blebs at their posterior ends. Some mortality was noted in culture by day 5, but most organisms were still alive when the cultures were terminated on day 7.

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  • In vitro excystation of Echinostoma paraensei (Digenea: Echinostomatidae) metacercariae assessed by light microscopy, morphometry and confocal laser scanning microscopy
    Joyce Souza, Juberlan Garcia, Renata H. Neves, José Roberto Machado-Silva, Arnaldo Maldonado
    Experimental Parasitology.2013; 135(4): 701.     CrossRef
  • 7,127 View
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Enterobius vermicularis egg positive rate in a primary school in Chungchongnam-do (Province) in Korea
Kyu-Jae Lee, In-Yong Lee, Kyung-il Im
Korean J Parasitol 2000;38(3):177-178.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.177

The egg positive rate of Enterobius vermicularis was investigated among students of a primary school and a kindergarten located in the rural area of Tangjin-gun, Chungchongnam-do in December, 1998. Of the 189 examinees, 28 (14.8%) were found to be infected with E. vermicularis by the adhesive cellotape anal swab method. The infection rates ranged from 4.2% to 26.1% among school children, and the highest rate was observed in children attending kindergarten. Three months after treatment with albendazole, four (14.3%) out of 28 infected children still remained infected with E. vermicularis. Through this survey, we were able to determine that E. vermicularis infection is still prevalent among children in rural areas of Korea.

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    Myoung-Ro Lee, Hee-Eun Shin, Seon-Ok Back, Young-Ju Lee, Jung-Won Ju, Chun Soon Park, Hee-Il Lee
    Parasites, Hosts and Diseases.2023; 61(1): 84.     CrossRef
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    Pichamon Janthu, Abdulhakam Dumidae, Chanakan Subkrasae, Jiranun Ardpairin, Saengchai Nateeworanart, Aunchalee Thanwisai, Apichat Vitta
    Parasitology Research.2022; 121(10): 2955.     CrossRef
  • Enterobius vermicularis infections in Iraq
    H. S. Al-Warid, A. Q. I. Alqaisi, I. M. Al Saqur, H. S. Al-Bahadely
    Helminthologia.2022; 59(4): 364.     CrossRef
  • Enterobius vermicularis Infection among Preschool Children: A 12-Year (2008-2019) Survey in Large Cities and Provinces of the Republic of Korea
    Hyejoo Shin, Bong-Kwang Jung, Seungwan Ryoo, Sooji Hong, Taehee Chang, Jiyeon Park, Keon Hoon Lee, Jeonggyu Lee, Jae Young Park, Hoo-Gn Jeoung, Jae Hyun Cho, Jong-Yil Chai
    The Korean Journal of Parasitology.2021; 59(4): 421.     CrossRef
  • The Prevalence of Intestinal Parasite Infection in El Behara Schoolchildren.
    Eman H. Radwan, Amel Abd El Rahman Hassan, Wael M. Lotfy, Ahmed Abd El-Mawgood, Hala M. Mashaal, Patricio De los RÃos
    International Journal of Limnology.2019; 1(1): 33.     CrossRef
  • Pinworm (Enterobius vermicularis) Infection among Primary Level Government School Children of Chhampi, Lalitpur District, Nepal
    Karuna Khadka, Mahendra Maharjan
    National Journal of Health Sciences.2018; 3(2): 46.     CrossRef
  • Impact of health education on the prevalence of enterobiasis in Korean preschool students
    In-Soon Kang, Dong-Hee Kim, Hye-Gyung An, Hyun-Mi Son, Min Kyoung Cho, Mi-Kyung Park, Shin Ae Kang, Bo Young Kim, Hak Sun Yu
    Acta Tropica.2012; 122(1): 59.     CrossRef
  • Comparison of Egg Positive Rates ofEnterobius vermicularisamong Preschool Children in Three Korean Localities
    Sung-Hee Hong, Sang-Eun Lee, Young-Il Jeong, Won-Ja Lee, Shin-Hyeong Cho
    The Korean Journal of Parasitology.2011; 49(4): 441.     CrossRef
  • Prevalence of Enterobius vermicularis Infection among Preschool Children in Kindergartens of Taipei City, Taiwan in 2008
    Tso-Kang Chang, Chien-Wei Liao, Ying-Chieh Huang, Chun-Chao Chang, Chia-Mei Chou, Hsin-Chieh Tsay, Alice Huang, Shu-Fen Guu, Ting-Chang Kao, Chia-Kwung Fan
    The Korean Journal of Parasitology.2009; 47(2): 185.     CrossRef
  • Egg positive rate of Enterobius vermicularis among preschool children in Cheongju, Chungcheongbuk-do, Korea
    Seokha Kang, Hyeong Kyu Jeon, Keeseon S. Eom, Joong-Ki Park
    The Korean Journal of Parasitology.2006; 44(3): 247.     CrossRef
  • A survey of Enterobius vermicularis infection among children on western and southern coastal islands of the Republic of Korea
    Jae-Hwan Park, Eun-Taek Han, Won-Hee Kim, Eun-Hee Shin, Sang-Mee Guk, Jae-Lip Kim, Jong-Yil Chai
    The Korean Journal of Parasitology.2005; 43(4): 129.     CrossRef
  • Enterobius vermicularisinfection among population of General Mansilla, Argentina
    Betina C Pezzani
    World Journal of Gastroenterology.2004; 10(17): 2535.     CrossRef
  • Egg positive rate of Enterobius vermicularis of primary school children in Geoje island
    Bong Jin Kim, Bo Young Lee, Hyun Kee Chung, Young Sun Lee, Kun Hee Lee, Hae Jin Chung, Mee Sun Ock
    The Korean Journal of Parasitology.2003; 41(1): 75.     CrossRef
  • Enterobius vermicularis egg positive rates in primary school children in Gangwon-do (Province), Korea
    Kyu-Jae Lee, Yung-Kyum Ahn, Yong-Suk Ryang
    The Korean Journal of Parasitology.2001; 39(4): 327.     CrossRef
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Intestinal parasite infections at an institution for the handicapped in Korea
Jongweon Lee, Gab-Man Park, Du-Ho Lee, Soon-Jung Park, Tai-Soon Yong
Korean J Parasitol 2000;38(3):179-181.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.179

Stool and cellotape anal swab examinations were carried out in August 1997 on handicapped people at an institution located in Chorwon-gun, Kangwon-do, Korea. A total of 112 stool samples (78 males and 34 females) revealed three cases of Trichuris trichiura infection and one case of Enterobius vermicularis infection. Other helminth eggs were not detected. The overall prevalence rate was 35.7% (38.5% for males and 29.4% for females). More than two different kinds of parasites were found in 42.0% of the positive stool samples (17 cases). The infection rates for protozoan cysts are as follow: Entamoeba coli (25.0%), E. histolytica (1.8%), Endolimax nana (21.4%), Iodoamoeba b?tschlii (1.8%) and Giardia lamblia (0.9%). In cellotape anal swab examinations (165 samples), the prevalence rate of E. vermicularis was 20.6% (25.7% of males and 9.6% of females). In conclusion, the handicapped people in the institution showed higher infection rates of protozoan parasites and E. vermicularis, possibly due to more accessibility to the infection.

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    Murat Saygın, Selahattin Aydemir, Abdurrahman Ekici, Hasan Yılmaz
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    Hyejoo Shin, Bong-Kwang Jung, Seungwan Ryoo, Sooji Hong, Taehee Chang, Jiyeon Park, Keon Hoon Lee, Jeonggyu Lee, Jae Young Park, Hoo-Gn Jeoung, Jae Hyun Cho, Jong-Yil Chai
    The Korean Journal of Parasitology.2021; 59(4): 421.     CrossRef
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    Sung-Tae Hong, Tai-Soon Yong
    Infection & Chemotherapy.2020; 52(3): 427.     CrossRef
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    Sima Rasti, Mohsen Arbabi, Hossein Hooshyar
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    Mehdi Sharif, Ahmad Daryani, Fatemeh Asgarian, Mohtaram Nasrolahei
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    Heejeong Youn
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    Cecilia Ximénez, Patricia Morán, Liliana Rojas, Alicia Valadez, Alejandro Gómez
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A small-scale survey of intestinal parasite infections among children and adolescents in Legaspi city, the Philippines
Kyu-Jae Lee, Yung-Kyum Ahn, Tai-Soon Yong
Korean J Parasitol 2000;38(3):183-185.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.183

To determine the status of infection caused by intestinal parasites among children and adolescents living in Legaspi city, the Philippines, we performed a small survey by fecal examination for helminth ova and protozoan cysts with formalin-ether concentration method. Of the 64 examinees, the infection rate was 78.1%. The infection rates of primary school children, preschool children and adolescents were 95.5%, 64.7% and 87.5%, respectively. The infection rate in urban areas was 56%, and 92.3% in rural areas. The infection rates were 51% with Trichuris trichiura, 40% with Ascaris lumbricoides, 23.4% with hookworm, 15.6% with Iodamoeba butschlii, 14.1% with Endolimax nana, 9.4% with Entamoeba coli and 7.8% with Giardia lamblia. There were 33 cases with multiple infection (51.6%). Mixed infection with more than 3 parasites was observed in 15 cases, all of them being children and adolescents living in rural areas. By this survey, it was conjectured that helminthic infection is prevalent among children and adolescents in Legaspi, Philippines.

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    Audrey N. Schuetz, Bobbi S. Pritt, Andrew M. Schreiner
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    J.V. Mbuh, N.H. Ntonifor, J. Ojong
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    Natasa Miladinovic-Tasic, Suzana Tasic, Ivana Kranjcic-Zec, Gordana Tasic, Aleksandar Tasic, Ivan Tasic
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    Antonio Montresor, Dai Tran Cong, Mouth Sinuon, Reiko Tsuyuoka, Chitsavang Chanthavisouk, Hanne Strandgaard, Raman Velayudhan, Corinne M. Capuano, Tuan Le Anh, Ah S. Tee Dató, Simon Brooker
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  • Infection status of intestinal parasites in children living in residential institutions in Metro Manila, the Philippines
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  • The intestinal parasite infection status of inhabitants in the Roxas city, the Philippines
    Bong-Jin Kim, Mee-Sun Ock, Dong-Il Chung, Tai-Soon Yong, Kyu-Jae Lee
    The Korean Journal of Parasitology.2003; 41(2): 113.     CrossRef
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Examination of gastrointestinal helminth in livestock grazing in grassland of Bangladesh
M. Motahar-Hussain Mondal, M-Khyrul Islam, Jin Hur, John-Hwa Lee, Byeong-Kirl Baek
Korean J Parasitol 2000;38(3):187-190.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.187

To determine association of grassland with parasitic diseases of livestock in Bangladesh, the 'Tracer' animals (two cow calves and two goats) were released for a month in a grassland used for communal grazing of livestock near school premise in Kanthal, Trishal, Mymensingh, Bangladesh. After slaughtering of the tracer animals, their gastrointestinal tract examination revealed six species of nematode and one cestode. The nematode species were Haemonchus contortus, Trichostrongylus axei, Mecistocirrus digitatus, Oesophagostomum spp., Trichuris spp. and Bunostomum sp. The cestode was one of the genus Moniezia. With this preliminary study, grasslands are thought to be one of the main sources of gastrointestinal parasitic diseases of livestock in Bangladesh.

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The 10 kDa protein of Taenia solium metacestodes shows genus specific antigenicity
Seung-Kyu Park, Doo-Hee Yun, Joon-Yong Chung, Yoon Kong, Seung-Yull Cho
Korean J Parasitol 2000;38(3):191-194.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.191

Genus specific antigenicity of the 10 kDa protein in cyst fluid (CF) of Taenia solium metacestodes was demonstrated by comparative immunoblot analysis. When CFs from taeniid metacestodes of T. saginata, T. solium, T. taeniaeformis and T. crassiceps were probed with specific monoclonal antibody (mAb) raised against 150 kDa protein of T. solium metacestodes, specific antibody reactions were observed in 7 and 10 kDa proteins of T. solium and in 7/8 kDa of T. saginata, T. taeniaeformis and T. crassiceps. The mAb did not react with any protein in hydatid fluid of Echinococcus granulosus and E. multilocularis. This result revealed that the 10 kDa peptide of T. solium metacestodes and its equivalent proteins of different Taenia metacestodes are genus specific antigens that are shared among different Taenia species.

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Differential expression of the 27 kDa cathepsin L-like cysteine protease in developmental stages of Spirometra erinacei
Yoon Kong, Doo-Hee Yun, Seung-Yull Cho, Woon-Mok Sohn, Young-Bae Chung, Shin-Yong Kang
Korean J Parasitol 2000;38(3):195-199.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.195

The 27 kDa cathepsin L-like cysteine protease of Spirometra erinacei plerocercoid is known to play an important function in tissue penetration, nutrient uptake and immune modulation in human sparganosis. In the present study, the expression of this enzyme was examined at different developmental stages of S. erinacei including immature egg, coracidium, plerocercoid in tadpole and rat, and adult. Proteolytic activity against carboxybenzoyl-phenylalanyl-arginyl-7-amino-4-methylcoumarin was detected in the extracts of coracidia and plerocercoid while no activity was observed in those of immature egg and adult. The specific activity in coracidial extracts was lower than that in the plerocercoid. Reverse transcription-polymerase chain reaction and Northern blot analysis demonstrated that the gene was expressed in the coracidium and plerocercoid but not in immature egg and adult. These results suggest that the 27 kDa cysteine protease is only expressed in the stages involving active migration of the parasite in the host tissue.

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Original Article
Chromosomes of the liver fluke, Clonorchis sinensis
Gab-Man Park, Kyung-il Im, Sun Huh, Tai-Soon Yong
Korean J Parasitol 2000;38(3):201-206.
Published online September 30, 2000
DOI: https://doi.org/10.3347/kjp.2000.38.3.201

A karyological study was carried out in order to compared the chromosome numbers, chromosome morphologies and karyotypes of the oriental liver fluke, Clonorchis sinensis (Trematoda: Opisthorchiidae), collected from Korea and China. Chromosome preparations were made by means of air-drying method. The chromosome number was 2n=56 in both Korean and Chinese flukes, and chromosomes were divided into two groups based on this size; consisting of 8 pairs of large and 20 pairs of small chromosomes. However, the karyotypes showed some differences between Korean and Chinese flukes. The karyotype of liver flukes from Korea consisted of three metacentric pairs, one meta-/submetacentric pair, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes. On the other hand, liver flukes from China consisted of two metacentric pairs, two meta-/submetacentric pairs, 16 submetacentric pairs and eight subtelocentric pairs of chromosomes.

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