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"Dermatophagoides farinae"

Original Article

Enzymatic Activities of Allergen Extracts from Three Species of Dust Mites and Cockroaches Commonly Found in Korean Home
Kyoung Yong Jeong, Chungryul Kim, Tai-Soon Yong
Korean J Parasitol 2010;48(2):151-155.
Published online June 17, 2010
DOI: https://doi.org/10.3347/kjp.2010.48.2.151

Allergen extracts from dust mites and cockroaches commonly found in Korean homes were used to evaluate their enzymatic activity as they are believed to influence allergenicity. Allergen extracts were prepared from 3 dust mite species (Dermatophagoides farinae, D. pteronyssinus, and Tyrophagus putrescentiae) and 3 cockroach species (Blattella germanica, Periplaneta americana, and P. fuliginosa) maintained in the Korea National Arthropods of Medical Importance Resource Bank. Proteins were extracted in PBS after homogenization using liquid nitrogen. The activities of various enzymes were investigated using the API Zym system. No significant difference in phosphatase, lipase, or glycosidase activity was observed among the 6 allergen extracts, but much difference was observed in protease activity. Protease activity was assessed in more detail by gelatin zymography and the EnzChek assay. Extract from T. putrescentiae showed the highest protease activity, followed by those of the cockroach extracts. Extracts from D. farinae and D. pteronyssinus showed only weak protease activity. Gelatinolytic activity was detected mainly in a 30-kDa protein in D. farinae, a 28-kDa protein in D. pteronyssinus, a > 26-kDa protein in T. putrescentiae, a > 20-kDa protein in B. germanica, and a > 23-kDa protein in P. americana and P. fuliginosa. The information on various enzymatic activities obtained in this study may be useful for future studies. In particular, the strong protease activity found in cockroach extracts could contribute to sensitization to cockroach allergens, which is known to be associated with the development of asthma.

Citations

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  • B-cell antibody class switchings are pressuromodulated events: Part II, gene recombination
    Hemant Sarin
    Translational Medicine Communications.2018;[Epub]     CrossRef
  • Allergenic Characterization of a Novel Allergen, Homologous to Chymotrypsin, from German Cockroach
    Kyoung Yong Jeong, Mina Son, Jae-Hyun Lee, Chein-Soo Hong, Jung-Won Park
    Allergy, Asthma & Immunology Research.2015; 7(3): 283.     CrossRef
  • Current Status of Standardization of Inhalant Allergen Extracts in Korea
    Kyoung Yong Jeong, Jae-Hyun Lee, Eun-Jin Kim, Joo-Shil Lee, Sang-Heon Cho, Soo-Jong Hong, Jung-Won Park
    Allergy, Asthma & Immunology Research.2014; 6(3): 196.     CrossRef
  • Preparation and Characterization of an Extract of German Cockroach From a Korean Source
    Kyoung Yong Jeong, Soo-Young Choi, Jae-Hyun Lee, Joo-Shil Lee, Tai-Soon Yong, Chein-Soo Hong, Jung-Won Park
    Allergy, Asthma & Immunology Research.2013; 5(2): 102.     CrossRef
  • The Effects of Storage Conditions on the Stability of House Dust Mite Extracts
    Kyoung Yong Jeong, Soo-Young Choi, In-Soo Han, Jae-Hyun Lee, Joo-Shil Lee, Chein-Soo Hong, Jung-Won Park
    Allergy, Asthma & Immunology Research.2013; 5(6): 397.     CrossRef
  • House Dust Mite Allergy in Korea: The Most Important Inhalant Allergen in Current and Future
    Kyoung Yong Jeong, Jung-Won Park, Chein-Soo Hong
    Allergy, Asthma & Immunology Research.2012; 4(6): 313.     CrossRef
  • Role of Cockroach Proteases in Allergic Disease
    Kristen Page
    Current Allergy and Asthma Reports.2012; 12(5): 448.     CrossRef
  • Optimization of Allergen Standardization
    Kyoung Yong Jeong, Chein-Soo Hong, Joo-Shil Lee, Jung-Won Park
    Yonsei Medical Journal.2011; 52(3): 393.     CrossRef
  • 9,144 View
  • 82 Download
  • Crossref

Brief Communication

Validity of MAST-CLA for diagnosis of arthropod allergy using receiver operating characteristic (ROC) analysis
Joon-Soo Park, Hae-Seon Nam, Yong-Bae Kim, Young-Jin Choi, Sang-Han Lee, Sung-Ho Kim
Korean J Parasitol 2007;45(3):239-243.
Published online September 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.3.239

Many allergists are currently focusing on the development of new diagnostic tools, and are attempting to improve both the sensitivity and specificity. A multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) is one of the most popular diagnostic tools used in the Republic of Korea. However, there remains controversy among allergists with regard to the cut-off point for a positive result. The present study was conducted in order to determine the validity of MAST-CLA as compared with that of the skin prick test, with particular emphasis on arthropod allergens, on the basis of percentage agreement rates and κ-values, and also to suggest the optimal positive cut-off points using receiver operating characteristic (ROC) curves. The study was conducted with 97 subjects (54 men, 43 women). Optimal individual cut-off points were calculated as follows; class II for Dermatophagoides farinae, class I for Dermatophagoides pteronyssinus, and trace for a cockroach mix. These findings suggest that attempting to apply optimal individual cut-off points will be a good way of improving diagnostic tests, particularly MAST-CLA.

Citations

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  • Performance of EAST in diagnosing inhalant allergens in children with allergic rhinitis
    P. Naina, Susmitha Karunasree Perumalla, Richa Gupta, John Antony Jude Prakash
    Indian Journal of Medical Microbiology.2022; 40(4): 593.     CrossRef
  • Comparison Study between MAST CLA and OPTIGEN
    Jin Kook Kim, Yeo-Min Yoon, Won-Jong Jang, Yeon-Joo Choi, Seok-Chan Hong, Jae Hoon Cho
    American Journal of Rhinology & Allergy.2011; 25(4): e156.     CrossRef
  • 7,703 View
  • 58 Download
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Original Articles

This experiment was undertaken to screen the acaricidal effects of herb essential oils (pennyroyal, ylang ylang, citronella, lemon grass, tea tree, and rosemary) at different doses (0.1, 0.05, 0.025, 0.0125, and 0.00625 ?l/cm2) and exposure times (5, 10, 20, 20, 30 and 60 min) on house dust mites Dermatophgoides farinae and D. pteronyssinus. The most effective acaricidal components of pennyroyal (Mentha pulegium) were analyzed using a gas chromatography-mass spectrometer (GC-MS). Of these essential oils, the most effective was pennyroyal, which is composed essentially of pulegone (> 99%), at a dose of 0.025 ?l/cm2 which at an exposure time of 5 min killed more than 98% of house dust mites. In the pennyroyal fumigation test, the closed method was more effective than the open method and maximum acaricidal effect was 100% at 0.025 ?l/cm2, 60 min. The results show that herb essential oils, in particular, pennyroyal was proved to have potent acaricidal activity.

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    Pharmaceutics.2022; 14(8): 1587.     CrossRef
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  • Anticholinergic, antidiabetic and antioxidant activities of Anatolian pennyroyal (Mentha pulegium)-analysis of its polyphenol contents by LC-MS/MS
    İlhami Gülçin, Ahmet C. Gören, Parham Taslimi, Saleh H. Alwasel, Omer Kılıc, Ercan Bursal
    Biocatalysis and Agricultural Biotechnology.2020; 23: 101441.     CrossRef
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Effectiveness of education for control of house dust mites and cockroaches in Seoul, Korea
Kyoung Yong Jeong, In-Yong Lee, Jongweon Lee, Han-Il Ree, Chein-Soo Hong, Tai-Soon Yong
Korean J Parasitol 2006;44(1):73-79.
Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.73

We evaluated the efficacy of health education in reducing indoor arthropod allergens in Seoul. The mite control measures comprised the use of mite-proof mattress and pillow coverings, regular washing of potentially infested materials, maintenance of a low humidity, removal of carpets, and frequent vacuum cleaning. Cockroach control measures included trapping, application of insecticides, and protecting food. Of 201 homes enrolled in October 1999, 63 volunteers were included in a 2-year follow-up survey between April 2000 and January 2002. Before intervention, the density of mites/g of dust varied greatly; 27.1/g in children's bedding, 20/g in adult bedding, 7.2/g on the floors of children's bedrooms, 6.8/g in sofas, 5.9/g on the floors of adult's bedrooms, 3.9/g on living room floors, 3.7/g in carpets, and 1.9 mites/g on kitchen floors. The predominant mite species and house percentages infested were; Dermatophagoides farinae 93%, D. pteronyssinus 9%, and Tyrophagus putrescentiae 8%. Comparing 1999 and 2001 infestations, before and after 25 mo of education, mite abundance was reduced by 98%, from 23.7 to 0.57 mites/g of dust. In 1999, cockroaches were detected in 62% homes: 36% Blattella germanica and 35% Periplaneta spp., including 9% double infestations of B. germanica and P. americana. Following intervention, cockroach infestation rates decreased to 22% of houses in 2000 and 23% in 2001. We conclude that continuous and repetitive health education resulted in the effective control of domestic arthropods.

Citations

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    Frontiers in Allergy.2023;[Epub]     CrossRef
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    Allergy, Asthma & Immunology Research.2013; 5(2): 102.     CrossRef
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    Tai-Soon Yong, Kyoung Yong Jeong
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  • 96 Download
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