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"Guangyou Yang"

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"Guangyou Yang"

Brief Communication

Sequence Analysis of cytb Gene in Echinococcus granulosus from Western China
Xiuqin Zhong, Ning Wang, Dandan Hu, Jiahai Wang, Tianyu Liu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Guangyou Yang
Korean J Parasitol 2014;52(2):205-209.
Published online April 18, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.2.205

Echinococcus granulosus is the causative agent of cystic echinococcosis with medical and veterinary importance in China. Our main
objective
was to discuss the genotypes and genetic diversity of E. granulosus present in domestic animals and humans in western China. A total of 45 hydatid cyst samples were collected from sheep, humans, and a yak and subjected to an analysis of the sequences of mitochondrial cytochrome b (cytb) gene. The amplified PCR product for all samples was a 1,068 bp band. The phylogenetic analysis showed that all 45 samples were identified as E. granulosus (genotype G1). Ten haplotypes were detected among the samples, with the main haplotype being H1. The haplotype diversity was 0.626, while the nucleotide diversity was 0.001. These results suggested that genetic diversity was low among our samples collected from the west of China based on cytb gene analysis. These findings may provide more information on molecular characteristics of E. granulosus from this Chinese region.

Citations

Citations to this article as recorded by  Crossref logo
  • Sequence analysis of the cytb gene of Mesocestoides Vaillant, 1863 tetrathyridia from small mammals of the Russian Far East
    N.A. Pospekhova, V.V. Pereverzeva, N.E. Dokuchaev, A.A. Primak
    Proceedings of the Zoological Institute RAS.2025; 329(1): 64.     CrossRef
  • G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq
    Musafer H. Al-Ardi
    Journal of Veterinary Science.2024;[Epub]     CrossRef
  • Phylogeny and population structure of Echinococcus granulosus (sensu stricto) based on full-length cytb-nad2-atp6 mitochondrial genes – First report from Sialkot District of Pakistan
    Mughees Aizaz Alvi, Rana Muhammad Athar Ali, Li Li, Muhammad Saqib, Warda Qamar, Ali Hassan, Muzafar Ghafoor, Siddiq Ur Rahman, Muhammad Umar Zafar Khan, Bao-Quan Fu, Youyu Liu, Hong Yin, Hong-Bin Yan, Wan-Zhong Jia
    Molecular and Biochemical Parasitology.2023; 253: 111542.     CrossRef
  • Survey and Molecular Characterization of Echinococcus granulosus sensu stricto from Livestock and Humans in the Altai Region of Xinjiang, China
    Baoping Guo, Li Zhao, Lu Zhao, Rongsheng Mi, Xu Zhang, Bingjie Wang, Gang Guo, Yuan Ren, Wenjing Qi, Zhuangzhi Zhang
    Pathogens.2023; 12(1): 134.     CrossRef
  • Update on the genetic diversity and population structure of Echinococcus granulosus in Gansu Province, Tibet Autonomous Region, and Xinjiang Uygur Autonomous Region, Western China, inferred from mitochondrial cox1, nad1, and nad5 sequences
    Nigus Abebe Shumuye, Li Li, John Asekhaen Ohiolei, Sayed Ajmal Qurishi, Wen-Hui Li, Nian-Zhang Zhang, Yan-Tao Wu, Yao-Dong Wu, Sheng-Zhi Gao, Fu-Heng Zhang, Xue-Qi Tian, Wen-Jun Tian, Yong Fu, Xie-Zhong Wang, Yong-Hong Pan, Fang Zhan, Lin-Sheng Zhang, Min
    Parasitology Research.2023; 122(5): 1107.     CrossRef
  • GENETIC ANALYSIS OF SUMATRAN ELEPHANTS IN SEBLAT NATURAL ECOTOURISM PARK BASED ON PARTIAL OF MITOCHONDRIAL CYTOCHROME B GENE
    SIPRIYADI, YANSEN, ARDEA BUJANA, ENI SURYANTI, MUHAMMAD CAHYADI, RICHI YULIAVIAN KUSMINANTO, CHOIRUL MUSLIM, TETI NAIBAHO, MONICA ANGGRAINI
    Malaysian Applied Biology.2022; 51(3): 117.     CrossRef
  • Food-borne zoonotic echinococcosis: A review with special focus on epidemiology
    Mughees Aizaz Alvi, Abdullah F. Alsayeqh
    Frontiers in Veterinary Science.2022;[Epub]     CrossRef
  • Haplotype comparisons of Echinococcus granulosus sensu lato via mitochondrial gene sequences (co1, cytb, nadh1) among Pakistan and its neighbouring countries
    Aisha Khan, Haroon Ahmed, Sami Simsek, Khuram Shahzad, Figen Celik, Muhammad Sohail Afzal, Mobushir Riaz Khan, Hua Liu, Yujuan Shen, Jianping Cao
    Parasitology.2021; 148(9): 1019.     CrossRef
  • Genotypes of Echinococcus isolated from domestic livestock in Kazakhstan
    A.M. Abdybekova, Z. Zhang, A.A. Sultanov, A.A. Abdibayeva, A.A. Zhaksylykova, S.M. Junisbayeva, M.Zh. Aubakirov, G.D. Akhmetova, P.R. Torgerson
    Journal of Helminthology.2020;[Epub]     CrossRef
  • Molecular characterization of human echinococcosis in Sichuan, Western China
    Jingye Shang, Guangjia Zhang, Wenjie Yu, Wei He, Qian Wang, Bo Zhong, Qi Wang, Sha Liao, ruirui Li, Fan Chen, Yan Huang
    Acta Tropica.2019; 190: 45.     CrossRef
  • Genetic characterization ofEchinococcusisolates from various intermediate hosts in the Qinghai-Tibetan Plateau Area, China
    Xiumin Han, Yingna Jian, Xueyong Zhang, Liqing Ma, Wenjun Zhu, Qigang Cai, Shile Wu, Xiangqian Wang, Bingqiang Shi
    Parasitology.2019; 146(10): 1305.     CrossRef
  • Genetic variation of Echinococcus spp. in yaks and sheep in the Tibet Autonomous Region of China based on mitochondrial DNA
    John Asekhaen Ohiolei, Chen-Yang Xia, Li Li, Jian-Zhi Liu, Wen-Qiang Tang, Yan-Tao Wu, Danqulamu, Guo-Qiang Zhu, Bin Shi, Bao-Quan Fu, Hong Yin, Hong-Bin Yan, Wan-Zhong Jia
    Parasites & Vectors.2019;[Epub]     CrossRef
  • Mitochondrial genome data confirm that yaks can serve as the intermediate host of Echinococcus canadensis (G10) on the Tibetan Plateau
    Yantao Wu, Li Li, Guoqiang Zhu, Wenhui Li, Nianzhang Zhang, Shuangnan Li, Gang Yao, Wenjun Tian, Baoquan Fu, Hong Yin, Xingquan Zhu, Hongbin Yan, Wanzhong Jia
    Parasites & Vectors.2018;[Epub]     CrossRef
  • Genetic diversity of three Chinese native sheep breeds
    G. -X. E, Y. -Fu. Huang, Y. -Ju Zhao, J. -N. He
    Russian Journal of Genetics.2017; 53(1): 118.     CrossRef
  • Cytochrome b conservation between six camel breeds reared in Egypt
    Othman E. Othman, Heba A.M. Abd El-Kader, Sally S. Alam, Sekena H. Abd El-Aziem
    Journal of Genetic Engineering and Biotechnology.2017; 15(1): 1.     CrossRef
  • Microdiversity of Echinococcus granulosus sensu stricto in Australia
    C. A. ALVAREZ ROJAS, D. EBI, C. G. Gauci, J. P. SCHEERLINCK, M. WASSERMANN, D. J. JENKINS, M. W. LIGHTOWLERS, T. ROMIG
    Parasitology.2016; 143(8): 1026.     CrossRef
  • Surveillance of Echinococcus isolates from Qinghai, China
    Junying Ma, Hu Wang, Gonghua Lin, Fang Zhao, Chao Li, Tongzuo Zhang, Xiao Ma, Yongguo Zhang, Zhibin Hou, Huixia Cai, Peiyun Liu, Yongshun Wang
    Veterinary Parasitology.2015; 207(1-2): 44.     CrossRef
  • 10,787 View
  • 94 Download
  • 21 Web of Science
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Original Articles
Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening
Yan Fu, Jingchao Lan, Xuhang Wu, Deying Yang, Zhihe Zhang, Huaming Nie, Rong Hou, Runhui Zhang, Wanpeng Zheng, Yue Xie, Ning Yan, Zhi Yang, Chengdong Wang, Li Luo, Li Liu, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Guangyou Yang
Korean J Parasitol 2014;52(1):21-26.
Published online February 19, 2014
DOI: https://doi.org/10.3347/kjp.2014.52.1.21

Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.

Citations

Citations to this article as recorded by  Crossref logo
  • Molecular Discovery of Filarial Nematode DNA in an Endangered Wild Pinniped (Galapagos Sea Lion, Zalophus wollebaeki)
    Isabella G. Livingston, Taylor M. Gregory, Eleanor C. Hawkins, Ashley Cave, Andrea Loyola, Shelly L. Vaden, Diane Deresienski, Marjorie Riofrío‐Lazo, Gregory A. Lewbart, Diego Páez‐Rosas, Matthew Breen
    Ecology and Evolution.2024;[Epub]     CrossRef
  • Development of TaqMan Quantitative PCR Assays for Duplex Detection of Dirofilaria immitis COI and Dog GAPDH from Infected Dog Blood
    In Young Oh, Kyung Tae Kim, Sun-Yeong Gwon, Ho Joong Sung
    The Korean Journal of Clinical Laboratory Science.2019; 51(1): 64.     CrossRef
  • Brown Spider (Loxosceles) Venom Toxins as Potential Biotools for the Development of Novel Therapeutics
    Daniele Chaves-Moreira, Fernando Hitomi Matsubara, Zelinda Schemczssen-Graeff, Elidiana De Bona, Vanessa Ribeiro Heidemann, Clara Guerra-Duarte, Luiza Helena Gremski, Carlos Chávez-Olórtegui, Andrea Senff-Ribeiro, Olga Meiri Chaim, Raghuvir Krishnaswamy A
    Toxins.2019; 11(6): 355.     CrossRef
  • Development of Real-time PCR Assays for Detection ofDirofilaria immitisfrom Infected Dog Blood
    In Young Oh, Kyung Tae Kim, Jin Hyun Jun, Jae-Ho Shin, Ho Joong Sung
    Korean Journal of Clinical Laboratory Science.2016; 48(2): 88.     CrossRef
  • 9,398 View
  • 84 Download
  • 3 Web of Science
  • Crossref
Genetic Variation of Taenia Pisiformis Collected from Sichuan, China, Based on the Mitochondrial Cytochrome b gene
Deying Yang, Yongjun Ren, Yan Fu, Yue Xie, Huaming Nie, Xiang Nong, Xiaobin Gu, Shuxian Wang, Xuerong Peng, Guangyou Yang
Korean J Parasitol 2013;51(4):449-452.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.449

Taenia pisiformis is one of the most important parasites of canines and rabbits. T. pisiformis cysticercus (the larval stage) causes severe damage to rabbit breeding, which results in huge economic losses. In this study, the genetic variation of T. pisiformis was determined in Sichuan Province, China. Fragments of the mitochondrial cytochrome b (cytb) (922 bp) gene were amplified in 53 isolates from 8 regions of T. pisiformis. Overall, 12 haplotypes were found in these 53 cytb sequences. Molecular genetic variations showed 98.4% genetic variation derived from intra-region. FST and Nm values suggested that 53 isolates were not genetically differentiated and had low levels of genetic diversity. Neutrality indices of the cytb sequences showed the evolution of T. pisiformis followed a neutral mode. Phylogenetic analysis revealed no correlation between phylogeny and geographic distribution. These findings indicate that 53 isolates of T. pisiformis keep a low genetic variation, which provide useful knowledge for monitoring changes in parasite populations for future control strategies.

Citations

Citations to this article as recorded by  Crossref logo
  • Sequence analysis of the cytb gene of Mesocestoides Vaillant, 1863 tetrathyridia from small mammals of the Russian Far East
    N.A. Pospekhova, V.V. Pereverzeva, N.E. Dokuchaev, A.A. Primak
    Proceedings of the Zoological Institute RAS.2025; 329(1): 64.     CrossRef
  • A survey of morphological, molecular, and histopathological characteristics of Taenia pisiformis metacestode in Egyptian rabbits (Oryctolagus cuniculus)
    Refaat Ras, Amanallah El-Bahrawy, Adel Abdelkhalek, Florica Morariu, Ayman N. Elsayed, Doaa S. Nouh, Anamaria Plesko, Marius Stelian Ilie, Manar AbdelMageed
    Frontiers in Veterinary Science.2025;[Epub]     CrossRef
  • Infection of Egyptian domestic rabbits, Oryctolagus cuniculus, with Cysticercus pisiformis (Cestoda: Taeniidae): morphological, molecular, and histopathological diagnostic tools
    Soheir A. H. Rabie, Wafaa A. Abuelwafa, Mouchira M. Mohi Eldin, Nermean M. Hussein
    Journal of Parasitic Diseases.2024; 48(4): 810.     CrossRef
  • Morphometry, molecular identification and histopathology of Passalurus ambiguus Rudolphi, 1819 in domestic rabbits (Oryctolagus cuniculus) in Qena, Upper Egypt
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    Journal of Parasitic Diseases.2022; 46(2): 511.     CrossRef
  • Theileria, Hepatozoon and Taenia infection in great gerbils (Rhombomys opimus) in northwestern China
    Na Ji, Xueling Chen, Gang Liu, Shanshan Zhao, Wenbo Tan, Guangyuan Liu, Jiangguo Zhang, Yuanzhi Wang
    International Journal for Parasitology: Parasites and Wildlife.2021; 15: 79.     CrossRef
  • Molecular Confirmation of Massive Taenia pisiformis Cysticercosis in One Rabbit in Poland
    Małgorzata Samorek-Pieróg, Jacek Karamon, Adam Brzana, Ewa Bilska-Zając, Jolanta Zdybel, Tomasz Cencek
    Pathogens.2021; 10(8): 1029.     CrossRef
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    Susana Remesar, Sabrina Castro-Scholten, Débora Jiménez-Martín, Leonor Camacho-Sillero, Patrocinio Morrondo, Carlos Rouco, Félix Gómez-Guillamón, David Cano-Terriza, Ignacio García-Bocanegra
    Preventive Veterinary Medicine.2021; 197: 105508.     CrossRef
  • Molecular Characterization of Enterocytozoon bieneusi in Domestic Rabbits (Oryctolagus cuniculus) in Northeastern China
    Xiao-Xuan Zhang, Jing Jiang, Ya-Nan Cai, Chun-Feng Wang, Peng Xu, Gui-Lian Yang, Quan Zhao
    The Korean Journal of Parasitology.2016; 54(1): 81.     CrossRef
  • Genetic Structure Analysis of Spirometra erinaceieuropaei Isolates from Central and Southern China
    Xi Zhang, Jing Cui, Li Na Liu, Peng Jiang, Han Wang, Xin Qi, Xing Qi Wu, Zhong Quan Wang, Wolfgang Arthofer
    PLOS ONE.2015; 10(3): e0119295.     CrossRef
  • 9,684 View
  • 92 Download
  • Crossref