Parasites, Hosts and Diseases

"Hee Jae Cha"

Brief Communication

Expression and Characterization of α-Methylacyl CoA Racemase from Anisakis simplex Larvae: Bong Jin Kim, Sun Mi Kim, Min Kyung Cho, Hak Sun Yu, Yong Seok Lee, Hee Jae Cha, Meesun Ock; Korean J Parasitol 2012;50(2):165-171.
Published online May 24, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.2.165

Abstract
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Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as α-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-40℃) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.

Citations

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The mitochondrial genome ofAncylostoma tubaeformefrom cats in China
X.L. Shi, Y.Q. Fu, A.Y. Abdullahi, M.W. Wang, F. Yang, X.G. Yu, W.D. Pan, X.X. Yan, J.X. Hang, P. Zhang, G.Q. Li
Journal of Helminthology.2018; 92(1): 22. CrossRef
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Progress in Lipid Research.2013; 52(2): 220. CrossRef

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Original Articles

A 24 kDa Excretory-Secretory Protein of Anisakis simplex Larvae Could Elicit Allergic Airway Inflammation in Mice: Hye-Kyung Park, Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Yun Seong Kim, Ki Uk Kim, Min Ki Lee, Mee Sun Ock, Hee Jae Cha, Hak Sun Yu; Korean J Parasitol 2011;49(4):373-380.
Published online December 16, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.4.373

Abstract
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We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-α (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses.

Citations

Citations to this article as recorded by

Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System
Jun Ho Choi, Ju Yeong Kim, Myung-hee Yi, Myungjun Kim, Tai-Soon Yong, Kalman Imre
BioMed Research International.2021;[Epub] CrossRef
Allergen-like Molecules from Parasites
Ju Yeong Kim, Myung-Hee Yi, Tai-Soon Yong
Current Protein & Peptide Science.2020; 21(2): 186. CrossRef
Research Note. Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish
M. Samir, M. A. Amin, A. O. Hassan, A. M. Merwad, M. A. I. Awadallah
Helminthologia.2015; 52(4): 375. CrossRef
Allergenicity of two Anisakis simplex allergens evaluated in vivo using an experimental mouse model
Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Maria Luisa Caballero, Teresa Perez-Pinar, Rosa Rodriguez-Perez, Mee Sun Ock, Hee Jae Cha, Yeon Chul Hong, Hak Sun Yu
Experimental Parasitology.2014; 146: 71. CrossRef
Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice
Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Hak Sun Yu
The Korean Journal of Parasitology.2013; 51(5): 583. CrossRef

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Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea: Jung Kim, Jin Ok Jo, Seon Hee Choi, Min Kyoung Cho, Hak Sun Yu, Hee Jae Cha, Meesun Ock; Korean J Parasitol 2011;49(2):139-144.
Published online June 14, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.2.139

Abstract
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The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Busan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of antibody positivity was 5.0% and 6.6% with ELISA against crude extracts and ESP, respectively. It was also revealed that infection occurred throughout all age groups and higher in females than in males. A specific protein band of 130 kDa was detected from 10 patients with western blot analysis against crude extract and ESP among those who showed positive results by ELISA. Our study showed for the first time the seroprevalence of anisakiasis in Korea. The allergen of 130 kDa can be a candidate for serologic diagnosis of anisakiasis.

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Eligia González-Rodríguez, Marta Rodero, J. Alberto Montoya-Alonso, Kevin M. Santana-Hernández, Myriam R. Ventura, Carmen Cuéllar, Eligia Rodríguez-Ponce
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Alvaro Daschner, Carmen Cuéllar
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S. Mattiucci, A. Colantoni, B. Crisafi, F. Mori‐Ubaldini, L. Caponi, P. Fazii, G. Nascetti, F. Bruschi
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International Journal of Molecular Sciences.2014; 15(3): 3624. CrossRef
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Naoki Hosoe, Haruhiko Ogata, Toshifumi Hibi
Parasitology International.2014; 63(1): 216. CrossRef
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A. H. Lin, I. Nepstad, E. Florvaag, E. Egaas, T. Van Do
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Occurrence of anisakid nematode larvae in chub mackerel (Scomber japonicus) caught off Korea
Tae-Jong Bak, Chan-Hyeok Jeon, Jeong-Ho Kim
International Journal of Food Microbiology.2014; 191: 149. CrossRef
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Young-Bae Chung, Jaechun Lee
Allergy, Asthma & Immunology Research.2014; 6(3): 228. CrossRef
Preventive and Therapeutic Effects of <italic>Anisakis simplex</italic> Larval Protein in a Mouse Model of Crohn’S Disease
Hee-Jae Cha, Mee Sun Ock
Kosin Medical Journal.2013; 28(2): 107. CrossRef
Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niter?i, Brazil
Israel Junior, Mauricio Vericimo, Luciana Cardoso, Sergio Clemente, Elmiro Nascimento, Gerlinde Teixeira
Acta Parasitologica.2013;[Epub] CrossRef
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Jeannette Guarner
Scientifica.2012; 2012: 1. CrossRef
Anisakidosis in Korea; Changes Over the Last Decade
Hee Jae Cha, Mee Sun Ock
Kosin Medical Journal.2012; 27(2): 73. CrossRef
Prevalence of Zoonotic Anisakid Nematodes in Inuit-Harvested Fish and Mammals from the Eastern Canadian Arctic
Erica L. Pufall, Andria Jones-Bitton, Scott A. McEwen, Tanya M. Brown, Victoria L. Edge, Jerzy Rokicki, Katarzyna Karpiej, Andrew S. Peregrine, Manon Simard
Foodborne Pathogens and Disease.2012; 9(11): 1002. CrossRef
A technique for the intra-gastric administration of live larvae of Anisakis simplex in mice
Israel Figueiredo, Luciana Cardoso, Gerlinde Teixeira, Leila Lopes, Sergio Carmona São Clemente, Mauricio Afonso Vericimo
Experimental Parasitology.2012; 130(3): 285. CrossRef

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Anisakis simplex Larvae: Infection Status in Marine Fish and Cephalopods Purchased from the Cooperative Fish Market in Busan, Korea: Seon Hee Choi, Jung Kim, Jin Ok Jo, Min Kyung Cho, Hak Sun Yu, Hee Jae Cha, Mee Sun Ock; Korean J Parasitol 2011;49(1):39-44.
Published online March 18, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.1.39

Abstract
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The infection status of marine fish and cephalopods with Anisakis simplex third stage larva (L3) was studied over a period of 1 year. A total of 2,537 specimens, which consisted of 40 species of fish and 3 species of cephalopods, were purchased from the Cooperative Fish Market in Busan, Korea, from August 2006 to July 2007. They were examined for A. simplex L3 from the whole body cavity, viscera, and muscles. A. simplex L3 were confirmed by light microscopy. The overall infection rate reached 34.3%, and average 17.1 larvae were parasitized per infected fish. Fish that recorded the highest infection rate was Lophiomus setigerus (100%), followed by Liparis tessellates (90%), Pleurogrammus azonus (90%), and Scomber japonicus (88.7%). The intensity of infection was the highest in Gadus macrocephalus (117.7 larvae per fish), followed by S. japonicus (103.9 larvae) and L. setigerus (54.2 larvae). Although abundance of A. simplex L3 was not seasonal in most of the fish species, 10 of the 16 selected species showed the highest abundance in February and April. A positive correlation between the intensity of L3 infection and the fish length was obvious in S. japonicus and G. macrocephalus. It was likely that A. simplex L3 are more frequently infected during the spring season in some species of fish. Our study revealed that eating raw or undercooked fish or cephalopods could still be a source of human infection with A. simplex L3 in Korea.

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