Parasites, Hosts and Diseases

"Hua Li"

Original Articles

Complete mitogenome sequence of Caryophyllaeus brachycollis (Cestoda: Caryophyllidae) from China: Characterization and phylogenetic analyses of Caryophyllidea: Yi-Liu Liu, Ya Zhang, Yi-Tian Fu, Guo-Hua Liu, Hui-Mei Wang, Yuan-Ping Deng; Parasites Hosts Dis 2025;63(4):317-326.
Published online November 19, 2025; DOI: https://doi.org/10.3347/PHD.25044

Caryophyllaeus brachycollis mainly parasitizes the intestines of globally distributed freshwater fishes, and infection causes significant economic losses to the aquaculture industry. However, data on the molecular epidemiology, population genetics, and systematics of C. brachycollis are scarce. In this study, we sequenced the complete mitogenome of C. brachycollis isolated from Beijing, China. This circular mitogenome comprised 14,273 bp, which was 231 bp shorter than that of C. brachycollis isolated from Wuhan, China. The mitogenome contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 2 noncoding regions. Bayesian inference revealed that C. brachycollis belonged to the family Caryophyllaeidae. The taxonomic status of C. brachycollis is controversial when based solely on morphological features. A comparative analysis of the mitogenome sequence obtained in this study revealed novel molecular markers for the accurate ascertainment of the phylogenetic position of this parasite.

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Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid: Yan Lu, Jia-Hui Sun, Li-Li Lu, Jia-Xu Chen, Peng Song, Lin Ai, Yu-Chun Cai, Lan-Hua Li, Shao-Hong Chen; Korean J Parasitol 2021;59(6):615-623.
Published online December 22, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.6.615

Human sparganosis is a food-borne parasitic disease caused by the plerocercoids of Spirometra species. Clinical diagnosis of sparganosis is crucial for effective treatment, thus it is important to identify sensitive and specific antigens of plerocercoids. The aim of the current study was to identify and characterize the immunogenic proteins of Spirometra erinaceieuropaei plerocercoids that were recognized by patient sera. Crude soluble extract of the plerocercoids were separated using 2-dimensional gel electrophoresis coupled with immunoblot and mass spectrometry analysis. Based on immunoblotting patterns and mass spectrometry results, 8 antigenic proteins were identified from the plerocercoid. Among the proteins, cysteine protease protein might be developed as an antigen for diagnosis of sparganosis.

Citations

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Lysine acetylation in the spargana of Spirometra mansoni: Insights into glycolysis and EF-hand domain proteins
Yuke Zeng, Asmaa M.I. Abuzeid, Qin Meng, Shuyu Chen, Xiaoruo Tan, Cuiqin Huang, Shiquan Lu, Teng Zhong, Yuanpeng Hu, Yisong Liu, Wei Liu
Acta Tropica.2025; : 107932. CrossRef
Establishment of Animal Infection Model of Spirometra Mansoni and Identification of Spirometra Mansoni by Enzyme-Linked Immunosorbent Assay
Anqi Luo, Shuyu Chen, Mingye He, Xiaoruo Tan, Zhikang Li, Wei Liu, Yisong Liu
Vector-Borne and Zoonotic Diseases.2024;[Epub] CrossRef
Immunoproteomics: Approach to Diagnostic and Vaccine Development
Virendra Supaji Gomase, Suchita Prabhakar Dhamane, Kiran Ramesh Kemkar, Pavan Ganpat Kakade, Abhay Dewappa Sakhare
Protein & Peptide Letters.2024; 31(10): 773. CrossRef

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A Novel Recombinant BCG Vaccine Encoding Eimeria tenella Rhomboid and Chicken IL-2 Induces Protective Immunity Against Coccidiosis: Qiuyue Wang, Lifeng Chen, Jianhua Li, Jun Zheng, Ning Cai, Pengtao Gong, Shuhong Li, He Li, Xichen Zhang; Korean J Parasitol 2014;52(3):251-256.
Published online June 26, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.3.251

Abstract
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A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene of E. tenella and chIL-2 gene were subcloned into integrative expression vector pMV361, producing vaccines rBCG pMV361-rho and pMV361-rho-IL2. Animal experiment via intranasal and subcutaneous route in chickens was carried out to evaluate the immune efficacy of the vaccines. The results indicated that these rBCG vaccines could obviously alleviate cacal lesions and oocyst output. Intranasal immunization with pMV361-rho and pMV361-rho-IL2 elicited better protective immunity against E. tenella than subcutaneous immunization. Splenocytes from chickens immunized with either rBCG pMV361-rho and pMV361-rho-IL2 had increased CD4⁺ and CD8⁺ cell production. Our data indicate recombinant BCG is able to impart partial protection against E. tenella challenge and co-expression of cytokine with antigen was an effective strategy to improve vaccine immunity.

Citations

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Alternatives to antibiotics against coccidiosis for poultry production: the relationship between immunity and coccidiosis management – a comprehensive review
Mohamed T. El-Saadony, Heba M. Salem, Marwa M. Attia, Nahed Yehia, Ahmed H. Abdelkader, Sara Abdel Mawgod, Nesma Mohamed Kamel, Samar Sami Alkafaas, Muslimah N. Alsulami, Ahmed Ezzat Ahmed, Dina Mostafa Mohammed, Ahmed M. Saad, Walid F.A. Mosa, Shaaban S.
Annals of Animal Science.2025;[Epub] CrossRef
Innovative prevention and control of coccidiosis: targeting sporogony for new control agent development
Dan Zhao, Jingxia Suo, Lin Liang, Ruiying Liang, Rongqiong Zhou, Jiabo Ding, Xianyong Liu, Xun Suo, Sixin Zhang, Xinming Tang
Poultry Science.2024; 103(12): 104246. CrossRef
Protective efficacy induced by Eimeria maxima rhomboid-like protein 1 against homologous infection
Mingyue Wang, Di Tian, Lixin Xu, Mingmin Lu, Ruofeng Yan, Xiangrui Li, Xiaokai Song
Frontiers in Veterinary Science.2023;[Epub] CrossRef
Protective Efficacy Induced by the Common Eimeria Antigen Elongation Factor 2 against Challenge with Three Eimeria Species in Chickens
Yuxuan Mi, Wenxi Ding, Lixin Xu, Mingmin Lu, Ruofeng Yan, Xiangrui Li, Xiaokai Song
Vaccines.2023; 12(1): 18. CrossRef
Vaccination Against Poultry Parasites
Ruediger Hauck, Kenneth S. Macklin
Avian Diseases.2023;[Epub] CrossRef
Coccidiosis: Recent Progress in Host Immunity and Alternatives to Antibiotic Strategies
Youngsub Lee, Mingmin Lu, Hyun S. Lillehoj
Vaccines.2022; 10(2): 215. CrossRef
A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens
ZhengQing Yu, SiYing Chen, JianMei Huang, WenXi Ding, YuFeng Chen, JunZhi Su, RuoFeng Yan, LiXin Xu, XiaoKai Song, XiangRui Li
Veterinary Research.2022;[Epub] CrossRef
Protective efficacy of new-generation anticoccidial vaccine candidates against Eimeria infection in chickens: A meta-analysis of challenge trials
Thanakarn Nasri, Somboon Sangmaneedet, Nguyen Hoai Nam, Kanlaya Worawong, Weerapol Taweenan, Peerapol Sukon
Veterinary Parasitology.2022; 306: 109724. CrossRef
BCG Vaccination: A potential tool against COVID-19 and COVID-19-like Black Swan incidents
Wenping Gong, Yingqing Mao, Yuexi Li, Yong Qi
International Immunopharmacology.2022; 108: 108870. CrossRef
Vaccines against chicken coccidiosis with particular reference to previous decade: progress, challenges, and opportunities
Tean Zaheer, Rao Zahid Abbas, Muhammad Imran, Asghar Abbas, Ali Butt, Sarfraz Aslam, Jameel Ahmad
Parasitology Research.2022; 121(10): 2749. CrossRef
Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens
Qiong Liu, Yanlong Jiang, Wentao Yang, Yongshi Liu, Chunwei Shi, Jing Liu, Xing Gao, Haibin Huang, Tianming Niu, Guilian Yang, Chunfeng Wang
Microbial Cell Factories.2020;[Epub] CrossRef
Recombinant Lactococcus lactis co-expressing dendritic cell target peptide and E. tenella 3-1E protein: immune response and efficacy against homologous challenge
Guanghao Li, Chunli Ma, Dian Wang, Wenjing Chen, Dexing Ma
Food and Agricultural Immunology.2020; 31(1): 379. CrossRef
Immune response and protective efficacy of recombinant Enterococcus faecalis displaying dendritic cell–targeting peptide fused with Eimeria tenella 3-1E protein
Wenjing Chen, Chunli Ma, Dian Wang, Guanghao Li, Dexing Ma
Poultry Science.2020; 99(6): 2967. CrossRef
Mucosal Delivery of a Self-destructing Salmonella-Based Vaccine Inducing Immunity Against Eimeria
Wei Kong, Xiao Wang, Emilia Fields, Blessing Okon, Mark C. Jenkins, Gary Wilkins, Matthew Brovold, Tiana Golding, Amanda Gonzales, Greg Golden, Josephine Clark-Curtiss, Roy Curtiss
Avian Diseases.2020;[Epub] CrossRef
A review of Eimeria antigen identification for the development of novel anticoccidial vaccines
J. Venkatas, M. A. Adeleke
Parasitology Research.2019; 118(6): 1701. CrossRef
Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection
Xinming Tang, Chaoyue Wang, Lin Liang, Dandan Hu, Sixin Zhang, Chunhui Duan, Jingxia Suo, Xianyong Liu, Xun Suo, Shangjin Cui
Parasites & Vectors.2019;[Epub] CrossRef
Four Cysteine Residues Contribute to Homodimerization of Chicken Interleukin-2
Chen Deng, Hailiang Tan, Hongda Zhou, Mengyun Wang, Yan Lü, Jiacui Xu, Huanmin Zhang, Limei Han, Yongxing Ai
International Journal of Molecular Sciences.2019; 20(22): 5744. CrossRef
Recombinant lactococcus lactis expressing Eimeria tenella AMA1 protein and its immunological effects against homologous challenge
Jian Li, Fen Wang, Chunli Ma, Yuchen Huang, Dian Wang, Dexing Ma
Experimental Parasitology.2018; 191: 1. CrossRef
Construction of Lactococcus lactis expressing secreted and anchored Eimeria tenella 3-1E protein and comparison of protective immunity against homologous challenge
Chunli Ma, Lili Zhang, Mingyang Gao, Dexing Ma
Experimental Parasitology.2017; 178: 14. CrossRef
Recombinant anticoccidial vaccines - a cup half full?
Damer P. Blake, Iván Pastor-Fernández, Matthew J. Nolan, Fiona M. Tomley
Infection, Genetics and Evolution.2017; 55: 358. CrossRef
Selecting Eimeria tenella-resistance markers by comparing resistant and susceptible groups of Jinghai Yellow chickens
Jingjing ZHANG, Yuxin LIN, Guojun DAI, Tingting AN, Mingming SUN, Genxi ZHANG, Kaizhou XIE, Jingyu WANG
TURKISH JOURNAL OF VETERINARY AND ANIMAL SCIENCES.2016; 40: 764. CrossRef
Effect of Diclazuril on the Bursa of Fabricius Morphology and SIgA Expression in Chickens Infected with <i>Eimeria tenella</i>
Bian-hua Zhou, Li-li Liu, Jeffrey Liu, Fu-wei Yuan, Er-jie Tian, Hong-wei Wang
The Korean Journal of Parasitology.2015; 53(6): 675. CrossRef

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Brief Communication

In Vitro Effects of SB202190 on Echinococcus granulosus: Hailong Lv, Siyuan Li, Jing Zhang, Weihua Liang, Xiaoling Mu, Yufeng Jiang; Korean J Parasitol 2013;51(2):255-258.
Published online April 25, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.2.255

Abstract
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Spillage of cyst contents during surgical operation is the major cause of recurrence after hydatid cyst surgery. Instillation of a scolicidal agent into a hepatic hydatid cyst is the most commonly employed measure to prevent this complication. SB202190 is a pyridinyl imidazole derivative and is known to be a specific inhibitor of p38 MAPK. In the present study, the scolicidal effect of SB202190 was investigated. Freshly isolated Echinococcus granulosus protoscolices were subjected to SB202190 treatment (10, 20, 40, and 80 ?M), and the effects on parasite viability were monitored by trypan blue staining. Corresponding effects were visualized by scanning and transmission electron microscopy. Dose-dependent protoscolex death within a few days of SB202190 treatment was observed. Although the in vitro scolicidal effect of SB202190 was satisfactory, the in vivo efficacy of this drug and also possible side effects remain to be further investigated.

Citations

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Chemical Composition and Antiparasitic Effects of Prangos ferulacea (L.) Lindl., Essential Oil against Echinococcus granulosus Protoscoleces
Hassan Abidi, Mohammadreza Bakhtiari, Mohammad Zoladl, Hossein Sadeghi Mansourkhani, Damoun Razmjoue, Mahmoud Bahmani
Anti-Infective Agents.2025;[Epub] CrossRef
Echinococcus granulosus promotes MAPK pathway-mediated osteoclast differentiation by inhibiting Nrf2 in osseous echinococcosis
Yaqing Liu, Jing Li, Zhendong Zhang, Qi Li, Yanhu Tian, Sibo Wang, Chenhui Shi, Haohao Sun
Veterinary Research.2025;[Epub] CrossRef
Propofol Induces the Expression of Nrf2 and HO-1 in Echinococcus granulosus via the JNK and p38 Pathway In Vitro
Guangyi Luo, Bin Ma, Yufeng Jiang, Hailong Lv
Tropical Medicine and Infectious Disease.2023; 8(6): 306. CrossRef
In vitro protoscolicidal effects of lithocholic acid on protoscoleces of Echinococcus granulosus and its mechanism
Ya Xu, Wenjuan Qing, Zhen Wang, Lin Chen, Linyao Wang, Hailong Lv, Yufeng Jiang
Experimental Parasitology.2022; 239: 108280. CrossRef
Transcriptome analysis uncovers the key pathways and candidate genes related to the treatment of Echinococcus granulosus protoscoleces with the repurposed drug pyronaridine
Yingfang Yu, Jun Li, Weisi Wang, Tian Wang, Wenjing Qi, Xueting Zheng, Lei Duan, Jiaxu Chen, Shizhu Li, Xiumin Han, Wenbao Zhang, Liping Duan
BMC Genomics.2021;[Epub] CrossRef
Ultrastructural characterization of the tegument in protoscoleces of Echinococcus ortleppi
Sebastián Miles, Javier Magnone, Joaquín García-Luna, María Eugenia Ancarola, Marcela Cucher, Sylvia Dematteis, Friedrich Frischknecht, Marek Cyrklaff, Gustavo Mourglia-Ettlin
International Journal for Parasitology.2021; 51(12): 989. CrossRef
Knock Down the Egp38 and Combine with Radiation to Increase Its Inhibitory Effect on Echinococcus granulosus
Pengfei Lu, Mengxiao Tian, Na Yi, Rui Mao, Hongzhi Qi, Liping Yang, Qin Zhou, Jun Li, Wenbao Zhang, Yongxing Bao
SSRN Electronic Journal .2021;[Epub] CrossRef
The combination of Cytokines and albendazole therapy for prophylaxis and treatment of experimental/hydatid cyst
M. Rahdar, A. Rafiei, R. Valipour- Nouroozi
Acta Tropica.2020; 201: 105206. CrossRef
In vitro scolicidal activity of Thymus capitatus Hoff. et Link. essential oil on Echinococcus granulosus protoscoleces
Amani Hizem, Selim M’rad, Myriam Oudni-M’rad, Habib Mezhoud, Hichem Ben Jannet, Guido Flamini, Kamel Ghedira, Hamouda Babba
Journal of Essential Oil Research.2020; 32(2): 178. CrossRef
Echinococcosis: Advances in the 21st Century
Hao Wen, Lucine Vuitton, Tuerhongjiang Tuxun, Jun Li, Dominique A. Vuitton, Wenbao Zhang, Donald P. McManus
Clinical Microbiology Reviews.2019;[Epub] CrossRef
Progress in the pharmacological treatment of human cystic and alveolar echinococcosis: Compounds and therapeutic targets
Mar Siles-Lucas, Adriano Casulli, Roberto Cirilli, David Carmena, Giovanna Raso
PLOS Neglected Tropical Diseases.2018; 12(4): e0006422. CrossRef
Efficiency of flubendazole-loaded mPEG-PCL nanoparticles: A promising formulation against the protoscoleces and cysts of Echinococcus granulosus
Mehdi Farhadi, Ali Haniloo, Kobra Rostamizadeh, Soghrat Faghihzadeh
Acta Tropica.2018; 187: 190. CrossRef
Protoscolicidal effects of chenodeoxycholic acid on protoscoleces of Echinococcus granulosus
Hongjuan Shi, Ying Lei, Bo Wang, Zhuo Wang, Guoqiang Xing, Hailong Lv, Yufeng Jiang
Experimental Parasitology.2016; 167: 76. CrossRef
Molecular Cloning and Characterization of a P38-Like Mitogen-Activated Protein Kinase from Echinococcus granulosus
Guodong Lü, Jing Li, Chuanshan Zhang, Liang Li, Xiaojuan Bi, Chaowang Li, Jinliang Fan, Xiaomei Lu, Dominique A. Vuitton, Hao Wen, Renyong Lin
The Korean Journal of Parasitology.2016; 54(6): 759. CrossRef
In vitro effects of vinegar on protoscolices of hydatid cyst
Reza Hajihossein, Zahra Eslamirad, Mahdi Mosayebi, Reza Ghasemikhah, Mojtaba Didehdar
Asian Pacific Journal of Tropical Disease.2015; 5(3): 210. CrossRef
In vivo and in vitro efficacies of mebendazole, mefloquine and nitazoxanide against cyst echinococcosis
Congshan Liu, Haobing Zhang, Jianhai Yin, Wei Hu
Parasitology Research.2015; 114(6): 2213. CrossRef
Intermittent Hypoxia-Induced Spinal Inflammation Impairs Respiratory Motor Plasticity by a Spinal p38 MAP Kinase-Dependent Mechanism
Adrianne G. Huxtable, Stephanie M.C. Smith, Timothy J. Peterson, Jyoti J. Watters, Gordon S. Mitchell
The Journal of Neuroscience.2015; 35(17): 6871. CrossRef

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Case Report

Clinical and Pathological Characteristics of Intraocular Cysticercosis: Juan-Juan Li, Li-Wei Zhang, Hua Li, Zhu-Lin Hu; Korean J Parasitol 2013;51(2):223-229.
Published online April 25, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.2.223

Abstract
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This study aimed to explore the clinical, radiological, and pathological characteristics of intraocular cysticercosis due to Taenia solium metacestode infection. Total 8 patients diagnosed with intraocular cysticercosis at the Red Cross Hospital of Yunnan Province, China were examined retrospectively. Patients with clear dioptic media had undergone fundus chromophotography. All patients underwent B ultrasonography of the ocular region (CT) successive scanning of the orbit and cerebral tissues. Parasites were extracted surgically and then examined pathologically. The fundus chromophotography showed a white and condensing scolex package in the vesicle. The B ultrasonic examination showed a vesicle-like echogenic mass in the vitreous chamber, in which the high-level echo spot was the cysticercus scolex. The pathological examinations showed that the vesicle wall exhibited hyaline degeneration, inflammatory cell infiltration, neuroglial fiber, and glial cell proliferation layers from the inside to the outside. The scolex is round and is composed of the outer tissue (the body wall) and the inner furrow tissue; these tissues migrated together. Primordially differentiated sucking discs were found in one case, but no hooklets were found. The inner scolex tissue was folded like a paper flower. The severity of intraocular disease is closely correlated with the pathophysiological processes of the cysticercus worm. Pathological examination of the intraocular lesions can help to evaluate the course of the disease as well as to provide a scientific basis for effective antiparasitic medication.

Citations

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Intraocular and neuro-cysticercosis with diffuse stromal choroiditis
Dipankar Das, Harsha Bhattacharjee, Kasturi Bhattacharjee, Manab Jyoti Barman, Saidul Islam, Bidhan Chandra Das, Saurabh Deshmukh, Apurba Deka, Palak Chirania, Nitu Kumari
Indian Journal of Pathology and Microbiology.2023; 66(1): 152. CrossRef
Cysticercosis in ophthalmology
Amar Pujari, Karthika Bhaskaran, Sujeeth Modaboyina, Deepshekhar Das, Gunjan Saluja, Asha Samdani, Pallavi Singh, Mandeep S Bajaj, Namrata Sharma
Survey of Ophthalmology.2022; 67(2): 544. CrossRef
A case of atypical peripheral intraocular cysticercosis
Xinlei Hao, Man Yuan, Liugui Chen, Wei Jin, Anhuai Yang
Indian Journal of Ophthalmology - Case Reports.2022; 2(1): 165. CrossRef
Clinicopathological study of parasitic lesions of the eye and ocular adnexa in a tertiary care ophthalmic center in South India
Meenakshi Mahesh, Marian Pauly, Shruthi M Krishna, M Raman, Jyotirmay Biswas
Indian Journal of Ophthalmology.2022; 70(5): 1713. CrossRef
Optical Coherence Tomography Features of Ocular Cysticercosis: A Review of Literature With Observer Variation
Abhishek Sheemar, Nripen Gaur, Pratima Singh Thakur, Pallvi Sharma, Brijesh Takkar, Sumeet Khanduja
Ophthalmic Surgery, Lasers and Imaging Retina.2022; 53(8): 446. CrossRef
Submacular Cysticercosis Successfully Treated through Conservative Management: Case Report
Renata García Franco, Alejandro Arias Gómez, Juvenal Guzman Cerda, Marlon García Roa, Paulina Ramirez Neria
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Two cases of brainstem neurocysticercosis removal: operative video
Xiaochun Zhao, Robert T. Wicks, Evgenii Belykh, Colin J. Przybylowski, Mohamed A. Labib, Peter Nakaji
Neurosurgical Focus: Video.2019; 1(2): V4. CrossRef
Analysis of Clinical Profile, Investigation, and Management of Ocular Cysticercosis Seen at a Tertiary Referral Centre
Sudha K. Ganesh, Priyanka
Ocular Immunology and Inflammation.2018; : 1. CrossRef
Subretinal Cysticercosis Extraction With Bimanual, 3-D, Heads–Up-Assisted Pars Plana Vitrectomy: Clinicopathological Correlation and Surgical Technique
Nandini Venkateswaran, Linda A. Cernichiaro-Espinosa, Catherin Negron, Brenda Fallas, Xiao Yi Zhou, Wilfredo Lara, Kara M. Cavuoto, Joshua Pasol, Janet L. Davis, Sander R. Dubovy, Audina M. Berrocal
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Update on pathology of ocular parasitic disease
Dipankar Das, Varsha Ramachandra, Saidul Islam, Harsha Bhattacharjee, Jyotirmay Biswas, Akanksha Koul, Panna Deka, Apurba Deka
Indian Journal of Ophthalmology.2016; 64(11): 794. CrossRef
Solitary Cysticercosis in Eye: Literature Review and A Hypothesis on Transmission of Infection
Priyadarshi Soumyaranjan Sahu, Akira Ito
Journal of Ocular Diseases and Therapeutics.2015; 3(1): 13. CrossRef
Toxic granulomatous anterior uveitis in live intracameral cysticercosis masquerading as leukocoria
Brijesh Takkar, Parijat Chandra, Kiran Kumar, Murugesan Vanathi
Canadian Journal of Ophthalmology.2014; 49(6): e140. CrossRef
Anterior segment optical coherence tomography of live ocular cysticercosis
Brijesh Takkar, Manzoor U Mehdi, Nasreen R Ahmed, Parijat Chandra, Murugesan Vanathi
Clinical & Experimental Ophthalmology.2014; 42(9): 896. CrossRef

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Original Articles

Genetic diversity of Acanthamoeba isolates from ocean sediments: Hua Liu, Young-Ran Ha, Sung-Tae Lee, Yean-Chul Hong, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2006;44(2):117-125.
Published online June 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.2.117

Abstract
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Genetic diversity of 18 Acanthamoeba isolates from ocean sediments was evaluated by comparing mitochondrial (mt) DNA RFLP, 18S rDNA sequences and by examining their cytopathic effects on human corneal epithelial cells versus reference strains. All isolates belonged to morphologic group II. Total of 16 restriction phenotypes of mtDNA from 18 isolates demonstrated the genetic diversity of Acanthamoeba in ocean sediments. Phylogenetic analysis using 18s rDNA sequences revealed that the 18 isolates were distinct from morphological groups I and III. Fifteen isolates showed close relatedness with 17 clinical isolates and A. castellanii Castellani and formed a lineage equivalent to T4 genotype of Byers' group. Two reference strains from ocean sediment, A. hatchetti BH-2 and A. griffini S-7 clustered unequivocally with these 15 isolates. Diversity among isolates was also evident from their cytopathic effects on human corneal cells. This is the first time describing Acanthamoeba diversity in ocean sediments in Korea.

Citations

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Detection of potentially pathogenic free-living amoebae from the Caspian Sea and hospital ward dust of teaching hospitals in Guilan, Iran
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Nuclear Group I introns with homing endonuclease genes in Acanthamoeba genotype T4
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European Journal of Protistology.2018; 66: 26. CrossRef
Molecular detection of Acanthamoeba spp., Naegleria fowleri and Vermamoeba (Hartmannella) vermiformis as vectors for Legionella spp. in untreated and solar pasteurized harvested rainwater
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Parasites & Vectors.2016;[Epub] CrossRef
Metagenomic characterization of viral communities in Goseong Bay, Korea
Jinik Hwang, So Yun Park, Mirye Park, Sukchan Lee, Yeonhwa Jo, Won Kyong Cho, Taek-Kyun Lee
Ocean Science Journal.2016; 51(4): 599. CrossRef
Pandoraviruses: Amoeba Viruses with Genomes Up to 2.5 Mb Reaching That of Parasitic Eukaryotes
Nadège Philippe, Matthieu Legendre, Gabriel Doutre, Yohann Couté, Olivier Poirot, Magali Lescot, Defne Arslan, Virginie Seltzer, Lionel Bertaux, Christophe Bruley, Jérome Garin, Jean-Michel Claverie, Chantal Abergel
Science.2013; 341(6143): 281. CrossRef
Mycobacterium gilvum Illustrates Size-Correlated Relationships between Mycobacteria and Acanthamoeba polyphaga
Otmane Lamrabet, Michel Drancourt
Applied and Environmental Microbiology.2013; 79(5): 1606. CrossRef
Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida)
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Molecular Phylogeny of Acanthamoeba
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The Korean Journal of Parasitology.2009; 47(Suppl): S21. CrossRef
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Evaluation of taxonomic validity of four species of Acanthamoeba: A. divionensis, A. paradivionensis, A. mauritaniensis, and A. rhysodes, inferred from molecular analyses: Hua Liu, Eun-Kyung Moon, Hak-Sun Yu, Hae-Jin Jeong, Yeon-Chul Hong, Hyun-Hee Kong, Dong-Il Chung; Korean J Parasitol 2005;43(1):7-13.
Published online March 20, 2005; DOI: https://doi.org/10.3347/kjp.2005.43.1.7

Abstract
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The taxonomy of Acanthamoeba spp., an amphizoic amoeba which causes granulomatous amoebic encephalitis and chronic amoebic keratitis, has been revised many times. The taxonomic validity of some species has yet to be assessed. In this paper, we analyzed the morphological characteristics, nuclear 18s rDNA and mitochondrial 16s rDNA sequences and the Mt DNA RFLP of the type strains of four Acanthamoeba species, which had been previously designated as A. divionensis, A. parasidionensis, A. mauritaniensis, and A. rhysodes. The four isolates revealed characteristic group II morphology. They exhibited 18S rDNA sequence differences of 0.2-1.1% with each other, but more than 2% difference from the other compared reference strains. Four isolates formed a different clade from that of A. castellanii Castellani and the other strains in morphological group II on the phylogenetic tree. In light of these results, A. paradivionensis, A. divionensis, and A. mauritaniensis should be regarded as synonyms for A. rhysodes.

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