Excretory-secretory products (ESP) of T. vaginalis have been shown to inhibit sperm motility, viability, and functional integrity, leading to a decreased fertilization rate in vitro. This study investigated whether T. vaginalis induce apoptosis and ultrastructural changes of sperm using flow cytometry and electron microscopy. Incubation of sperm with T. vaginalis ESP increased phosphatidylserine externalization and DNA fragmentation, and decreased mitochondrial membrane potential. Transmission electron microscopy of sperm incubated with ESP revealed abnormal features such as distorted heads, broken necks, and acrosomes exocytosis. This is the first report that demonstrates a direct impact of T. vaginalis ESP on sperm apoptosis and architecture in vitro.
Citations
Citations to this article as recorded by
The possible pathogenic mechanisms of microorganisms in infertility: a narrative review Zahra Chegini, Amin Khoshbayan, Milad Kashi, Raha Zare Shahraki, Mojtaba Didehdar, Aref Shariati Archives of Microbiology.2025;[Epub] CrossRef
Lactobacillus species in the female genital tract are thought to act as a barrier to infection. Several studies have demonstrated that lactobacilli can adhere to vaginal epithelial cells. However, little is known about how the adherence of lactobacilli to vaginal epithelial cells affects the acidity, cell viability, or proliferation of the lactobacilli themselves or those of vaginal epithelial cells. Lactobacillus acidophilus was co-cultured with immortalized human vaginal epithelial cells (MS74 cell line), and the growth of L. acidophilus and the acidity of the culture medium were measured. MS74 cell density and viability were also assessed by counting cell numbers and observing the cell attachment state. L. acidophilus showed exponential growth for the first 6 hr until 9 hr, and the pH was maintained close to 4.0-5.0 at 24 hr after culture, consistent with previous studies. The growth curve of L. acidophilus or the pH values were relatively unaffected by co-culture with MS74 cells, confirming that L. acidophilus maintains a low pH in the presence of MS74 cells. This co-culture model could therefore potentially be used to mimic vaginal conditions for future in vitro studies. On the other hand, MS74 cells co-cultured with L. acidophilus more firmly attached to the culture plate, and a higher number of cells were present compared to cells cultured in the absence of L. acidophilus. These results indicate that L. acidophilus increases MS74 cell proliferation and viability, suggesting that lactobacilli may contribute to the healthy environment for vaginal epithelial cells.
Citations
Citations to this article as recorded by
Effect of Pediococcus acidilactici and mango seed polyphenols on the fermentative profile of the indigestible fraction of yam bean Alba Cecilia Durán-Castañeda, Adela Yolanda Bueno-Durán, Manuel Iván Girón-Pérez, Juan Arturo Ragazzo-Sánchez, Jorge Alberto Sánchez-Burgos, Sonia Guadalupe Sáyago-Ayerdi, Victor Manuel Zamora-Gasga Food Research International.2024; 178: 113970. CrossRef
In Vitro Digestion of Vacuum-Impregnated Yam Bean Snacks: Pediococcus acidilactici Viability and Mango Seed Polyphenol Bioaccessibility Alba Cecilia Durán-Castañeda, Adela Yolanda Bueno-Durán, Manuel Iván Girón-Pérez, Juan Arturo Ragazzo-Sánchez, Jorge Alberto Sánchez-Burgos, Sonia Guadalupe Sáyago-Ayerdi, Victor Manuel Zamora-Gasga Microorganisms.2024; 12(10): 1993. CrossRef
Safety Assessment and Probiotic Potential Comparison of Bifidobacterium longum subsp. infantis BLI-02, Lactobacillus plantarum LPL28, Lactobacillus acidophilus TYCA06, and Lactobacillus paracasei ET-66 Jui-Fen Chen, Ko-Chiang Hsia, Yi-Wei Kuo, Shu-Hui Chen, Yen-Yu Huang, Ching-Min Li, Yu-Chieh Hsu, Shin-Yu Tsai, Hsieh-Hsun Ho Nutrients.2023; 16(1): 126. CrossRef
In-depth insights into cervicovaginal microbial communities and hrHPV infections using high-resolution microbiome profiling Mariano A. Molina, Karolina M. Andralojc, Martijn A. Huynen, William P. J. Leenders, Willem J. G. Melchers npj Biofilms and Microbiomes.2022;[Epub] CrossRef
Self-Emulsified Nanoemulsion for Vaginal Administration: In Vitro Study of Effect on Lactobacillus acidophilus Prachi Jirwankar, Shruthi Gobbooru, Jun Shao Journal of Pharmaceutical Sciences.2020; 109(10): 3145. CrossRef
Effect of Lactobacillus acidophilus and Porphyromonas gingivalis on proliferation and apoptosis of gingival epithelial cells Jun-jun Zhao, Long Jiang, Ya-qin Zhu, Xi-ping Feng Advances in Medical Sciences.2019; 64(1): 54. CrossRef
Lactobacillus fermentum ATCC 23271 Displays In vitro Inhibitory Activities against Candida spp. Monique S. do Carmo, Francisca M. F. Noronha, Mariana O. Arruda, Ênnio P. da Silva Costa, Maria R. Q. Bomfim, Andrea S. Monteiro, Thiago A. F. Ferro, Elizabeth S. Fernandes, Jorge A. Girón, Valério Monteiro-Neto Frontiers in Microbiology.2016;[Epub] CrossRef
Natural antimicrobials subtilosin and lauramide arginine ethyl ester synergize with conventional antibiotics clindamycin and metronidazole against biofilms of Gardnerella vaginalis but not against biofilms of healthy vaginal lactobacilli Ammar Algburi, Anna Volski, Michael L. Chikindas Pathogens and Disease.2015;[Epub] CrossRef
Computational methodology for predicting the landscape of the human–microbial interactome region level influence Edgar D. Coelho, André M. Santiago, Joel P. Arrais, José Luís Oliveira Journal of Bioinformatics and Computational Biology.2015; 13(05): 1550023. CrossRef
Evaluation of antipathogenic activity and adherence properties of human Lactobacillus
strains for vaginal formulations M.C. Verdenelli, M.M. Coman, C. Cecchini, S. Silvi, C. Orpianesi, A. Cresci Journal of Applied Microbiology.2014; 116(5): 1297. CrossRef
Lactobacillus Decelerates Cervical Epithelial Cell Cycle Progression Katarina Vielfort, Linda Weyler, Niklas Söderholm, Mattias Engelbrecht, Sonja Löfmark, Helena Aro, Paul J. Galardy PLoS ONE.2013; 8(5): e63592. CrossRef
First
Prev
