Parasites, Hosts and Diseases

"Pongchai Harnyuttanakorn"

Original Articles

Unraveling Haplotype Diversity of the Apical Membrane Antigen-1 Gene in Plasmodium falciparum Populations in Thailand: Lalita Lumkul, Vorthon Sawaswong, Phumin Simpalipan, Morakot Kaewthamasorn, Pongchai Harnyuttanakorn, Sittiporn Pattaradilokrat; Korean J Parasitol 2018;56(2):153-165.
Published online April 30, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.2.153

Development of an effective vaccine is critically needed for the prevention of malaria. One of the key antigens for malaria vaccines is the apical membrane antigen 1 (AMA-1) of the human malaria parasite Plasmodium falciparum, the surface protein for erythrocyte invasion of the parasite. The gene encoding AMA-1 has been sequenced from populations of P. falciparum worldwide, but the haplotype diversity of the gene in P. falciparum populations in the Greater Mekong Subregion (GMS), including Thailand, remains to be characterized. In the present study, the AMA-1 gene was PCR amplified and sequenced from the genomic DNA of 65 P. falciparum isolates from 5 endemic areas in Thailand. The nearly fulllength 1,848 nucleotide sequence of AMA-1 was subjected to molecular analyses, including nucleotide sequence diversity, haplotype diversity and deduced amino acid sequence diversity and neutrality tests. Phylogenetic analysis and pairwise population differentiation (F_st indices) were performed to infer the population structure. The analyses identified 60 single nucleotide polymorphic loci, predominately located in domain I of AMA-1. A total of 31 unique AMA-1 haplotypes were identified, which included 11 novel ones. The phylogenetic tree of the AMA-1 haplotypes revealed multiple clades of AMA-1, each of which contained parasites of multiple geographical origins, consistent with the Fst indices indicating genetic homogeneity or gene flow among geographically distinct populations of P. falciparum in Thailand’s borders with Myanmar, Laos and Cambodia. In summary, the study revealed novel haplotypes and population structure needed for the further advancement of AMA-1-based malaria vaccines in the GMS.

Citations

Citations to this article as recorded by

Genetic diversity and natural selection of apical membrane antigen-1 (ama-1) in Cameroonian Plasmodium falciparum isolates
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Gene.2024; 894: 147956. CrossRef
Genetic polymorphism and natural selection of the erythrocyte binding antigen 175 region II in Plasmodium falciparum populations from Myanmar and Vietnam
Tuấn Cường Võ, Hương Giang Lê, Jung-Mi Kang, Haung Naw, Won Gi Yoo, Moe Kyaw Myint, Huynh Hong Quang, Byoung-Kuk Na
Scientific Reports.2023;[Epub] CrossRef
Genetic diversity of Plasmodium falciparum AMA-1 antigen from the Northeast Indian state of Tripura and comparison with global sequences: implications for vaccine development
Tulika Nirmolia, Md. Atique Ahmed, Vinayagam Sathishkumar, Nilanju P. Sarma, Dibya R. Bhattacharyya, Pradyumna K. Mohapatra, Devendra Bansal, Praveen K. Bharti, Rakesh Sehgal, Jagadish Mahanta, Ali A. Sultan, Kanwar Narain, Saurav J. Patgiri
Malaria Journal.2022;[Epub] CrossRef
Global diversity of the gene encoding the Pfs25 protein—a Plasmodium falciparum transmission-blocking vaccine candidate
Pornpawee Sookpongthai, Korawich Utayopas, Thassanai Sitthiyotha, Theerakamol Pengsakul, Morakot Kaewthamasorn, Kittikhun Wangkanont, Pongchai Harnyuttanakorn, Surasak Chunsrivirot, Sittiporn Pattaradilokrat
Parasites & Vectors.2021;[Epub] CrossRef
Diversify and Conquer: The Vaccine Escapism of Plasmodium falciparum
Alena Pance
Microorganisms.2020; 8(11): 1748. CrossRef
Plasmodium falciparum Blood Stage Antimalarial Vaccines: An Analysis of Ongoing Clinical Trials and New Perspectives Related to Synthetic Vaccines
David Ricardo Salamanca, Marcela Gómez, Anny Camargo, Laura Cuy-Chaparro, Jessica Molina-Franky, César Reyes, Manuel Alfonso Patarroyo, Manuel Elkin Patarroyo
Frontiers in Microbiology.2019;[Epub] CrossRef
Genotyping genetically heterogeneousCyclospora cayetanensisinfections to complement epidemiological case linkage
Joel L. N. Barratt, Subin Park, Fernanda S. Nascimento, Jessica Hofstetter, Mateusz Plucinski, Shannon Casillas, Richard S. Bradbury, Michael J. Arrowood, Yvonne Qvarnstrom, Eldin Talundzic
Parasitology.2019; 146(10): 1275. CrossRef
Reverse immunodynamics: a new method for identifying targets of protective immunity
Katrina J. Spensley, Paul S. Wikramaratna, Bridget S. Penman, Andrew Walker, Adrian L. Smith, Oliver G. Pybus, Létitia Jean, Sunetra Gupta, José Lourenço
Scientific Reports.2019;[Epub] CrossRef

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Allelic Diversity and Geographical Distribution of the Gene Encoding Plasmodium falciparum Merozoite Surface Protein-3 in Thailand: Vorthon Sawaswong, Phumin Simpalipan, Napaporn Siripoon, Pongchai Harnyuttanakorn, Sittiporn Pattaradilokrat; Korean J Parasitol 2015;53(2):177-187.
Published online April 22, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.2.177

Abstract
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Merozoite surface proteins (MSPs) of malaria parasites play critical roles during the erythrocyte invasion and so are potential candidates for malaria vaccine development. However, because MSPs are often under strong immune selection, they can exhibit extensive genetic diversity. The gene encoding the merozoite surface protein-3 (MSP-3) of Plasmodium falciparum displays 2 allelic types, K1 and 3D7. In Thailand, the allelic frequency of the P. falciparum msp-3 gene was evaluated in a single P. falciparum population in Tak at the Thailand and Myanmar border. However, no study has yet looked at the extent of genetic diversity of the msp-3 gene in P. falciparum populations in other localities. Here, we genotyped the msp-3 alleles of 63 P. falciparum samples collected from 5 geographical populations along the borders of Thailand with 3 neighboring countries (Myanmar, Laos, and Cambodia). Our study indicated that the K1 and 3D7 alleles coexisted, but at different proportions in different Thai P. falciparum populations. K1 was more prevalent in populations at the Thailand-Myanmar and Thailand-Cambodia borders, whilst 3D7 was more prevalent at the Thailand-Laos border. Global analysis of the msp-3 allele frequencies revealed that proportions of K1 and 3D7 alleles of msp-3 also varied in different continents, suggesting the divergence of malaria parasite populations. In conclusion, the variation in the msp-3 allelic patterns of P. falciparum in Thailand provides fundamental knowledge for inferring the P. falciparum population structure and for the best design of msp-3 based malaria vaccines.

Citations

Citations to this article as recorded by

Genetic diversity of Plasmodium falciparum AMA-1 antigen from the Northeast Indian state of Tripura and comparison with global sequences: implications for vaccine development
Tulika Nirmolia, Md. Atique Ahmed, Vinayagam Sathishkumar, Nilanju P. Sarma, Dibya R. Bhattacharyya, Pradyumna K. Mohapatra, Devendra Bansal, Praveen K. Bharti, Rakesh Sehgal, Jagadish Mahanta, Ali A. Sultan, Kanwar Narain, Saurav J. Patgiri
Malaria Journal.2022;[Epub] CrossRef
Genetic polymorphism of merozoite surface protein-3 in Myanmar Plasmodium falciparum field isolates
Hương Giang Lê, Thị Lam Thái, Jung-Mi Kang, Jinyoung Lee, Mya Moe, Tuấn Cường Võ, Haung Naw, Moe Kyaw Myint, Zaw Than Htun, Tong-Soo Kim, Ho-Joon Shin, Byoung-Kuk Na
Malaria Journal.2020;[Epub] CrossRef
Unraveling Haplotype Diversity of the Apical Membrane Antigen-1 Gene in Plasmodium falciparum Populations in Thailand
Lalita Lumkul, Vorthon Sawaswong, Phumin Simpalipan, Morakot Kaewthamasorn, Pongchai Harnyuttanakorn, Sittiporn Pattaradilokrat
The Korean Journal of Parasitology.2018; 56(2): 153. CrossRef
Genetic diversity of the merozoite surface protein-3 gene in Plasmodium falciparum populations in Thailand
Sittiporn Pattaradilokrat, Vorthon Sawaswong, Phumin Simpalipan, Morakot Kaewthamasorn, Napaporn Siripoon, Pongchai Harnyuttanakorn
Malaria Journal.2016;[Epub] CrossRef

11,971 View
114 Download
4 Web of Science
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Drug Resistance and in Vitro Susceptibility of Plasmodium falciparum in Thailand during 1988-2003: Nantana Suwandittakul, Wanna Chaijaroenkul, Pongchai Harnyuttanakorn, Mathirut Mungthin, Kesara Na Bangchang; Korean J Parasitol 2009;47(2):139-144.
Published online May 27, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.139

Abstract
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The aim of the present study was to investigate antimalarial drug pressure resulting from the clinical use of different antimalarials in Thailand. The phenotypic diversity of the susceptibility profiles of antimalarials, i.e., chloroquine (CQ), quinine (QN), mefloquine (MQ), and artesunate (ARS) in Plasmodium falciparum isolates collected during the period from 1988 to 2003 were studied. P. falciparum isolates from infected patients were collected from the Thai-Cambodian border area at different time periods (1988-1989, 1991-1992, and 2003), during which 3 different patterns of drug use had been implemented: MQ + sulphadoxine (S) + pyrimethamine (P), MQ alone and MQ + ARS, respectively. The in vitro drug susceptibilities were investigated using a method based on the incorporation of [³H] hypoxanthine. A total of 50 isolates were tested for susceptibilities to CQ, QN, MQ, and ARS. Of these isolates, 19, 16, and 15 were adapted during the periods 1988-1989, 1991-1993, and 2003, respectively. P. falciparum isolates collected during the 3 periods were resistant to CQ. Sensitivities to MQ declined from 1988 to 2003. In contrast, the parasite was sensitive to QN, and similar sensitivity profile patterns were observed during the 3 time periods. There was a significantly positive but weak correlation between the IC₅₀ values of CQ and QN, as well as between the IC₅₀ values of QN and MQ. Drug pressure has impact on sensitivity of P. falciparum to MQ. A combination therapy of MQ and ARS is being applied to reduce the parasite resistance, and also increasing the efficacy of the drug.

Citations

Citations to this article as recorded by

In vitro sensitivity of antimalarial drugs and correlation with clinico-parasitological response following treatment with a 3-day artesunate-mefloquine combination in patients with falciparum malaria along the Thai-Myanmar border
Phunuch Muhamad, Artitaya Thiengsusuk, Papichaya Phompradit, Kesara Na-Bangchang
Acta Tropica.2017; 166: 257. CrossRef
Ex vivo drug sensitivity profiles of Plasmodium falciparum field isolates from Cambodia and Thailand, 2005 to 2010, determined by a histidine-rich protein-2 assay
Stuart D Tyner, Chanthap Lon, Youry Se, Delia Bethell, Doung Socheat, Harald Noedl, Darapiseth Sea, Wichai Satimai, Kurt Schaecher, Wiriya Rutvisuttinunt, Mark M Fukuda, Suwanna Chaorattanakawee, Kritsanai Yingyuen, Siratchana Sundrakes, Panjaporn Chaicha
Malaria Journal.2012;[Epub] CrossRef
Pyronaridine-Artesunate versus Chloroquine in Patients with Acute Plasmodium vivax Malaria: A Randomized, Double-Blind, Non-Inferiority Trial
Yi Poravuth, Duong Socheat, Ronnatrai Rueangweerayut, Chirapong Uthaisin, Aung Pyae Phyo, Neena Valecha, B. H. Krishnamoorthy Rao, Emiliana Tjitra, Asep Purnama, Isabelle Borghini-Fuhrer, Stephan Duparc, Chang-Sik Shin, Lawrence Fleckenstein, Lorenz von S
PLoS ONE.2011; 6(1): e14501. CrossRef
Is it too soon to eliminate quinine?
Hubert Barennes, Leila M Srour, Eric Pussard
The Lancet Infectious Diseases.2010; 10(3): 141. CrossRef