Parasites, Hosts and Diseases

"Pyung-Rim Chung"

Original Article

Double-stranded RNA virus in Korean Isolate IH-2 of Trichomonas vaginalis: Jong Wook Kim, Pyung-Rim Chung, Myung-Ki Hwang, Eun Young Choi; Korean J Parasitol 2007;45(2):87-94.
Published online June 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.2.87

Abstract
PDF

In this study, we describe Korean isolates of Trichomonas vaginalis infected with double-stranded (ds) RNA virus (TVV). One T. vaginalis isolate infected with TVV IH-2 evidenced weak pathogenicity in the mouse assay coupled with the persistent presence of a dsRNA, thereby indicating a hypovirulence effect of dsRNA in T. vaginalis. Cloning and sequence analysis results revealed that the genomic dsRNA of TVV IH-2 was 4,647 bp in length and evidenced a sequence identity of 80% with the previously-described TVV 1-1 and 1-5, but only a 42% identity with TVV 2-1 and 3 isolates. It harbored 2 overlapping open reading frames of the putative capsid protein and dsRNA-dependent RNA polymerase (RdRp). As previously observed in the TVV isolates 1-1 and 1-5, a conserved ribosomal slippage heptamer (CCUUUUU) and its surrounding sequence context within the consensus 14-nt overlap implied the gene expression of a capsid protein-RdRp fusion protein, occurring as the result of a potential ribosomal frameshift event. The phylogenetic analysis of RdRp showed that the Korean TVV IH-2 isolate formed a compact group with TVV 1-1 and 1-5 isolates, which was divergent from TVV 2-1, 3 and other viral isolates classified as members of the Giardiavirus genus.

Citations

Citations to this article as recorded by

ՏՐԻԽՈՄՈՆԱՍ ՎԱԳԻՆԱԼԻՍ ՎԻՐՈՒՍԻ (TVV) ԱԶԴԵՑՈՒԹՅՈՒՆԸ ԿԱՆԱՆՑ ՄԻԶԱՍԵՌԱԿԱՆ ՏՐԻԽՈՄՈՆԻԱԶԻ ՎՐԱ
G.K. Ghardyan
MEDICINE, SCIENCE AND EDUCATION.2024; (37): 70. CrossRef
Microbial Matryoshka: Addressing the Relationship between Pathogenic Flagellated Protozoans and Their RNA Viral Endosymbionts (Family Totiviridae)
Alexandra Ibañez-Escribano, Maria Teresa Gomez-Muñoz, Marta Mateo, Cristina Fonseca-Berzal, Esperanza Gomez-Lucia, Raquel Garcia Perez, Jose M. Alunda, Javier Carrion
Veterinary Sciences.2024; 11(7): 321. CrossRef
Multiple Regulations of Parasitic Protozoan Viruses: A Double-Edged Sword for Protozoa
Zhiteng Zhao, Xin Li, Nan Zhang, Jianhua Li, Na Zhao, Mengyao Gao, Xichen Zhang, Xiaocen Wang, Panpan Zhao, Lu Li, Min Sun, Lili Cao, Pengtao Gong, Vinayaka R. Prasad
mBio.2023;[Epub] CrossRef
PROTOZOONLARIN VİRAL ENDOSİMBİYONTLARI
Ayşegül DAMLAPINAR, Kader YILDIZ
Veteriner Farmakoloji ve Toksikoloji Derneği Bülteni.2023; 14(1): 25. CrossRef
Discovery of a Novel Species of Trichomonasvirus in the Human Parasite Trichomonas vaginalis Using Transcriptome Mining
Austin Manny, Carrie Hetzel, Arshan Mizani, Max Nibert
Viruses.2022; 14(3): 548. CrossRef
A systematic review and meta-analysis on the global status of Trichomonas vaginalis virus in Trichomonas vaginalis
Saeed Bahadory, Selva Aminizadeh, Ali Taghipour, Farah Bokharaei-Salim, Khadijeh Khanaliha, Mohammad Hossein Razizadeh, Alireza Soleimani, Leila beikzadeh, Alireza Khatami
Microbial Pathogenesis.2021; 158: 105058. CrossRef
Biosynthesis Products of Pseudomonas sp. PS-17 Strain Metabolites. 1. Obtaining and Thermal Characteristics
Ihor Semeniuk, Viktoria Kochubei, Volodymyr Skorokhoda, Tetyana Pokynbroda, Halyna Midyana, Elena Karpenko, Viktor Melnyk
Chemistry & Chemical Technology.2020; 14(1): 26. CrossRef
Trichomonas vaginalis virus: a review of the literature
KJ Graves, AP Ghosh, PJ Kissinger, CA Muzny
International Journal of STD & AIDS.2019; 30(5): 496. CrossRef
Novel Partitivirus Enhances Virulence of and Causes Aberrant Gene Expression in Talaromyces marneffei
Susanna K. P. Lau, George C. S. Lo, Franklin W. N. Chow, Rachel Y. Y. Fan, James J. Cai, Kwok-Yung Yuen, Patrick C. Y. Woo, John W. Taylor
mBio.2018;[Epub] CrossRef
Trichomonas vaginalis infection in symbiosis with Trichomonasvirus and Mycoplasma
Raina Fichorova, Jorge Fraga, Paola Rappelli, Pier Luigi Fiori
Research in Microbiology.2017; 168(9-10): 882. CrossRef
Double-stranded RNA viral infection of Trichomonas vaginalis (TVV1) in Iranian isolates
Khadijeh Khanaliha, Hossein Masoumi-Asl, Farah Bokharaei-Salim, Azardokht Tabatabaei, Mehri Naghdalipoor
Microbial Pathogenesis.2017; 109: 56. CrossRef
Molecular characterization of double-stranded RNA virus in Trichomonas vaginalis Egyptian isolates and its association with pathogenicity
Eman K. El-Gayar, Amira B. Mokhtar, Wael A. Hassan
Parasitology Research.2016; 115(10): 4027. CrossRef
High rates of double-stranded RNA viruses and Mycoplasma hominis in Trichomonas vaginalis clinical isolates in South Brazil
Débora da Luz Becker, Odelta dos Santos, Amanda Piccoli Frasson, Graziela de Vargas Rigo, Alexandre José Macedo, Tiana Tasca
Infection, Genetics and Evolution.2015; 34: 181. CrossRef
Genetic characterization of three Cuban Trichomonas vaginalis virus. Phylogeny of Totiviridae family
Jorge Fraga, Lazara Rojas, Idalia Sariego, Ayme Fernández-Calienes
Infection, Genetics and Evolution.2012; 12(1): 113. CrossRef
Identification and characterization of a type III Trichomonas vaginalis virus in the protozoan pathogen Trichomonas vaginalis
Irina N. Bessarab, Rui Nakajima, Hsing-Wei Liu, Jung-Hsiang Tai
Archives of Virology.2011; 156(2): 285. CrossRef
Clinical Isolates of Trichomonas vaginalis Concurrently Infected by Strains of Up to Four Trichomonasvirus Species (Family Totiviridae)
Russell P. Goodman, Taylor S. Freret, Tomasz Kula, Alexander M. Geller, Megan W. T. Talkington, Vanessa Tang-Fernandez, Olimpia Suciu, Aleksander A. Demidenko, Said A. Ghabrial, David H. Beach, Bibhuti N. Singh, Raina N. Fichorova, Max L. Nibert
Journal of Virology.2011; 85(9): 4258. CrossRef

15,666 View
71 Download
Crossref

Brief Communication

Corbicula fluminea (Bivalvia: Corbiculidae): a possible second molluscan intermediate host of Echinostoma cinetorchis (Trematoda: Echinostomatidae) in Korea: Pyung-Rim Chung, Younghun Jung, Yun-Kyu Park, Myung-Gi Hwang, Chin-Thack Soh; Korean J Parasitol 2001;39(4):329-332.
Published online December 31, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.4.329

Abstract
PDF

More than 1,500 clams of Corbicula fluminea, the most favorable food source of freshwater bivalves in Korea, were collected from 5 localities to examine cercarial and metacercarial infection with Echinostoma cinetorchis. Although 3 clams infected with suspicious E. cinetorchis metacercariae out of 200 specimens collected at Kangjin, Chollanam-do were detected, no cercarial and metacercarial infections with E. cinetorchis were observed in field-collected Corbicula specimens. In the susceptibility experiments with laboratory-reared clams, those infected with miracidia of E. cinetorchis did not release their cercariae up to 60 days after infection. To confirm the identity of second intermediate host of E. cinetorchis experimentally, a total of 30 clams were exposed to the cercariae from Segmentina hemisphaerula that had been infected with miracidia of E. cinetorchis. The clams were susceptible to cercariae of E. cinetorchis with an infection rate of 93.3%. Metacercariae from clams taken more than 7 days after cercarial exposure were fed to rats (S/D strain), and adult worms of E. cinetorchis, characterized by 37-38 collar spines on the head crown, were recovered from the ileocecal regions. This is the first report of C. fluminea as a possible second intermediate host of E. cinetorchis.

Citations

Citations to this article as recorded by

Lower parasite pressure in invasive freshwater bivalves than in sympatric native Unionidae mussels in southern European lakes
Binglin Deng, Nicoletta Riccardi, Maria Urbańska, Timo J. Marjomäki, Wojciech Andrzejewski, Jouni Taskinen
Biological Invasions.2025;[Epub] CrossRef
The Nuclear Ribosomal Transcription Units of Two Echinostomes and Their Taxonomic Implications for the Family Echinostomatidae
Yu Cao, Ye Li, Zhong-Yan Gao, Bo-Tao Jiang
Biology.2025; 14(8): 1101. CrossRef
Infection of Corbicula clams by trematode cercariae in Myanmar
Alexander V. Kropotin, Yulia V. Bespalaya, Alexander V. Kondakov, Irina S. Khrebtova, Ilya V. Vikhrev, Ivan N. Bolotov
Ecologica Montenegrina.2023; 62: 1. CrossRef
Corbicula fluminea (Asian clam)
Uma Sabapathy Allen
CABI Compendium.2022;[Epub] CrossRef
Corbicula fluminalis
Fabiana Freitas
CABI Compendium.2022;[Epub] CrossRef
Parasites of Aquatic Exotic Invertebrates: Identification of Potential Risks Posed to the Great Lakes
Sergey E. Mastitsky, Alexander Y. Karatayev, Lyubov E. Burlakova
Human and Ecological Risk Assessment: An International Journal.2014; 20(3): 743. CrossRef
Korean molluscs as auxiliary hosts for parasites: A study of implications for pathogen transmission in a changing climate
Gab-Man Park
The Korean Journal of Malacology.2012; 28(1): 13. CrossRef
ENCYSTMENT AND METACERCARIAE DEVELOPMENT OF ECHINOSTOMA CINETORCHIS CERCARIAE IN AN IN VITRO CULTURE SYSTEM
Yun-Kyu Park, Myung-Ki Hwang, Pyung-Rim Chung
Journal of Parasitology.2006; 92(5): 1010. CrossRef
Host Specificity of Pisidium coreanum (Bivalvia: Sphaeriidae) to Larval Infection with a Human Intestinal Fluke Echinostoma cinetorchis (Trematoda: Echinostomatidae) in Korea
Y. K. Park, C. T. Soh, G. M. Park, M. K. Hwang, P. R. Chung
Journal of Parasitology.2006; 92(5): 1118. CrossRef

8,248 View
87 Download
Crossref

Original Articles

Austropeplea ollula (Pulmonata: Lymnaeidae): A new molluscan intermediate host of a human intestinal fluke, Echinostoma cinetorchis (Trematoda: Echinostomatidae) in Korea: Pyung-Rim Chung, Younghun Jung, Yun-Kyu Park, Myung-Ki Hwang; Korean J Parasitol 2001;39(3):247-253.
Published online September 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.3.247

Abstract
PDF

Three freshwater snail species of the family Lymnaeidae have been reported from Korea, Radix auricularia coreana, Austropeplea ollula and Fossaria truncatula. Out of 3 lymnaeid snail species, A. ollula was naturally infected with the Echinostoma cinetorchis cercariae (infection rate = 0.7%). In the experiments with the laboratory-bred snails, F. truncatula as well as A. ollula was also susceptible to the E. cinetorchis miracidia with infection rates of 25% and 40%, respectively. All of three lymnaeid snail species exposed to the E. cinetorchis cercariae were infected with the E. cinetorchis metacercariae. It is evident that A. ollula acts as the first molluscan intermediate host of E. cinetorchis in Korea, and F. truncatula may be a possible candidate for the first intermediate host of this intestinal fluke. Also, three lymnaeid snail species targeted were experimentally infected with E. cinetorchis metacercariae.

Citations

Citations to this article as recorded by

The Nuclear Ribosomal Transcription Units of Two Echinostomes and Their Taxonomic Implications for the Family Echinostomatidae
Yu Cao, Ye Li, Zhong-Yan Gao, Bo-Tao Jiang
Biology.2025; 14(8): 1101. CrossRef
Risk of invasion and disease transmission by the Australasian freshwater snail Orientogalba viridis (Lymnaeidae): a field and experimental study
Antonio A. Vázquez, Elodie Chapuis, Jorge Sánchez, Pilar Alda, Dominique Faugère, Mónica Sánchez, Léa Souq, Joaquín López-Soriano, Sergio Quiñonero-Salgado, Nicolás Bonel, Jean-Pierre Pointier, Annia Alba, Sylvie Hurtrez-Boussès
Parasites & Vectors.2024;[Epub] CrossRef
Morphology and Molecular Identification of Echinostoma revolutum and Echinostoma macrorchis in Freshwater Snails and Experimental Hamsters in Upper Northern Thailand
Preeyaporn Butboonchoo, Chalobol Wongsawad, Pheravut Wongsawad, Jong-Yil Chai
The Korean Journal of Parasitology.2020; 58(5): 499. CrossRef
Examining the phylogeny of the Australasian Lymnaeidae (Heterobranchia: Pulmonata: Gastropoda) using mitochondrial, nuclear and morphological markers
Louise Puslednik, Winston F. Ponder, Mark Dowton, Andrew R. Davis
Molecular Phylogenetics and Evolution.2009; 52(3): 643. CrossRef

7,835 View
79 Download
Crossref

Immunoelectron microscopic localization of partially purified antigens in adult Paragonimus iloktsuenensis: Ok-Ran Lee, Pyung-Rim Chung; Korean J Parasitol 2001;39(2):119-132.
Published online June 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.2.119

Abstract
PDF

An immunoelectron microscopy employing immunogold labeling method was performed to detect tissue origin of D1 fraction (D1A) among 5 antigenic protein fractions partially purified by DEAE-anion exchange chromatography from water-soluble crude antigen (PIWA) of adult Paragonimus iloktsuenensis. Immune reactions of adult worm tissues with rabbit serum immunoglobulin immunized with crude antigen (PI-Ig) and D1 antigen (D1-Ig), as well as rat serum immunoglobulin infected with P. iloktsuenensis were observed. D1A showed strong antigenicity in the intestinal epithelium of the worms during the early infection period of 2-4 weeks after infection. The vitellaria also showed stronger antigenicity than the other tissue sites in immune reaction of tissues against all immunoglobulins from 4 to 33 weeks after vitelline development. Therefore, it is suggested that D1A was mainly originated from the intestinal epithelial tissues before the development of vitelline gland of the parasites. Immuno-reactivity of two immunoglobulins (PI-Ig, D1-Ig) was significantly different in intestinal epithelial cytoplasmic protrusions (CP) and intestinal epithelial secretory granules (SG). In the experimental group with D1-Ig, gold particles were labeled significantly in CP than in SG when compared to the PI-Ig group. Thus, the major antigenic materials in D1 antigen having a strong antigenicity in the early infection period was considered to be originated from the intestinal epithelial tissue.

Citations

Citations to this article as recorded by

Nuclear ribosomal transcription units in Asian Paragonimus species (Paragonimidae: Platyhelminthes): genetic characteristics, polymorphism, and implications for intersuperfamilial phylogeny
Khue Thi Nguyen, Huong Thi Thanh Doan, Linh Thi Khanh Pham, Do Thi Roan, Takeshi Agatsuma, Pham Ngoc Doanh, Thanh Hoa Le
Parasitology Research.2024;[Epub] CrossRef
Mitogenomic and nuclear ribosomal transcription unit datasets support the synonymy of Paragonimus iloktsuenensis and P. ohirai (Paragonimidae: Platyhelminthes)
Thanh Hoa Le, Khue Thi Nguyen, Linh Thi Khanh Pham, Huong Thi Thanh Doan, Do Thi Roan, Xuyen Thi Kim Le, Takeshi Agatsuma, David Blair
Parasitology Research.2023; 122(7): 1531. CrossRef
Paragonimus westermani infection manifesting as a pulmonary cavity and adrenal gland mass: A case report
Yong Shik Kwon, Hye Won Lee, Hyun Jung Kim
Journal of Infection and Chemotherapy.2019; 25(3): 200. CrossRef
Ion-exchange protocol to obtain antigenic fractions with potential for serodiagnosis of strongyloidiasis
H. T. GONZAGA, C. VILA-VERDE, D. S. NUNES, V. S. RIBEIRO, J. P. CUNHA-JÚNIOR, J. M. COSTA-CRUZ
Parasitology.2013; 140(1): 69. CrossRef

6,990 View
46 Download
Crossref

Non-specific activation of mouse peritoneal macrophages by a freshwater ciliate, Tetrahymena pyriformis: Younghun Jung, Ki-Sun Kim, Pyung-Rim Chung; Korean J Parasitol 2000;38(2):65-74.
Published online June 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.2.65

Abstract
PDF

Toxoplasma-killing activities of mouse peritoneal macrophages activated by the extracts of Tetrahymena pyriformis (Korean and Chinese strains) were evaluated, and the active protein fractions from both strains were partially characterized by a method including chromatographies and SDS-PAGE. The first peak in Korean strain and the second peak in Chinese strain of T. pyriformis obtained by DEAE-Sephadex A-50 chromatography were most effective in the activation of macrophages to kill Toxoplasma gondii tachyzoites in vitro. Subsequent fractionations of obtained peak fractions were performed on a Sephadex G-200 gel. The first peaks fractionated from both strains of T. pyriformis had the highest toxoplasmacidal activities, and when subjected to the SDS-PAGE, one prominent band was visualized for each of the strains showing the same molecular weight of ca. 52.6 kDa. This active protein is suggested to be related to non-specific activation of mouse peritoneal macrophages.

7,066 View
67 Download

Karyotypes on three species of Chinese mesogastropod snails, Semisulcospira libertina, S. dolichostoma and Viviparus rivularis: Gab-Man Park, Jae-Jin Kim, Pyung-Rim Chung, Yong Wang, Duk-Young Min; Korean J Parasitol 1999;37(1):5-11.
Published online March 31, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.1.5

Abstract
PDF

Three species of the families Viviparidae and Pleuroceridae, the first intermediate host of paragonimiasis, metagonimiasis and echinostomiasis were studied cytologically. The observed diploid chromosome number was as follows: Semisulcospira libertina 36, S. dolichostoma 34, and Viviparus rivularis 64. The mitotic chromosome complement of S. libertina has nine metacentric pairs and nine submetacentric pairs, and S. dolichostoma has three metacentric pairs and 14 submetacentric pairs of chromosomes. Viviparus rivularis showed two metacentric pairs and 30 submetacentric pairs of chromosomes.

Citations

Citations to this article as recorded by

An adaptable chromosome preparation methodology for use in invertebrate research organisms
Longhua Guo, Alice Accorsi, Shuonan He, Carlos Guerrero-Hernández, Shamilene Sivagnanam, Sean McKinney, Matthew Gibson, Alejandro Sánchez Alvarado
BMC Biology.2018;[Epub] CrossRef
Blood glycemia-modulating effects of melanian snail protein hydrolysates in mice with type II diabetes
Jae-Suk Choi, Joo-Wan Kim, Jeong Been Park, Sang Eun Pyo, Yong-Ki Hong, Sae Kwang Ku, Mi-Ryung Kim
International Journal of Molecular Medicine.2017; 39(6): 1437. CrossRef
Karyotype of Arion vulgaris Moquin-Tandon, 1856 (Gastropoda: Pulmonata: Arionidae)
Alexandr V. Garbar, Natalia S. Kadlubovska
Folia Malacologica.2014;[Epub] CrossRef